FITC Anti-Hepatitis B Virus Surface Antigen (Ad/Ay) antibody (ab20930)

Reviews (1)Q&A (3)References (1)

Key features and details

  • FITC Goat polyclonal to Hepatitis B Virus Surface Antigen (Ad/Ay)
  • Suitable for: ELISA
  • Reacts with: Hepatitis B virus
  • Conjugation: FITC. Ex: 493nm, Em: 528nm
  • Isotype: IgG

Overview

  • Product name

    FITC Anti-Hepatitis B Virus Surface Antigen (Ad/Ay) antibody
    See all Hepatitis B Virus Surface Antigen (Ad/Ay) primary antibodies

  • Description

    FITC Goat polyclonal to Hepatitis B Virus Surface Antigen (Ad/Ay)

  • Host species

    Goat

  • Conjugation

    FITC. Ex: 493nm, Em: 528nm

  • Specificity

    Purified surface antigen. Monospecific, reacts only with Hepatitis B virus.

  • Tested applications

    Suitable for: ELISAmore details

  • Species reactivity

    Reacts with: Hepatitis B virus

  • Immunogen

    Hepatitis B surface antigen purified from human serum (mixture of subtypes ad & ay).

  • General notes

    Covalently coupled with high purity Isomer I of fluorescein isothiocyanate. Care is taken to ensure complete removal of any free fluorescein from the final product.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Associated products

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab20930 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
Use at an assay dependent concentration.
Notes
ELISA
Use at an assay dependent concentration.

Target

  • Relevance

    Hepatitis B Virus (HBV) infection induces a disease state characterised by liver damage, inflammation and viral persistence. Infection also increases the risk of hepatocellular carcinoma. HBV belongs to the Hepadnaviridae family of viruses. Its genome consists of partially double stranded circular DNA. The DNA is enclosed in a nucleocapsid, or core antigen (HBcAg), which is surrounded by a spherical envelope (surface antigen or HBsAg). The core antigen shares its sequences with the e antigen (HBeAg) but no cross reactivity between the two proteins has been observed. The HBV genome also encodes a DNA polymerase that also acts as a reverse transcriptase. Hepatitis B infection is normally diagnosed from serological tests that detect HBsAg but as the disease progresses this antigen may no longer be present in the blood and tests for HBcAg are used. If HBsAg can be detected in the blood for longer than six months, chronic hepatitis B is diagnosed. The antigenic determinant of the protein moiety of the HBsAg determines specific characteristics of different serotypes and provides the basis of immunodetection. HBsAg has antigenic heterogeneity, specifically, two pairs of sub specific determinants, d/y and w/r allow the following combinations: adw, ayw, adr, ayr.

  • Cellular localization

    Virion membrane

  • Database links

    see all

  • Alternative names

    • HBsAg antibody
    • HBV major surface antigen antibody
    • HBV surface antigen antibody
    • Hepatitis B Virus major surface antigen antibody
    • Major surface antigen antibody
    • S antibody
    see all

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (1)

Publishing research using ab20930? Please let us know so that we can cite the reference in this datasheet.

ab20930 has been referenced in 1 publication.

  • Ortega-Prieto AM  et al. 3D microfluidic liver cultures as a physiological preclinical tool for hepatitis B virus infection. Nat Commun 9:682 (2018). PubMed: 29445209

Customer reviews and Q&As

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1-4 of 4 Abreviews or Q&A

Flow Cytometry abreview for Anti-Hepatitis B Virus Surface Antigen (Ad/Ay) antibody (FITC)

 Inconclusive
Abreviews
Application
Flow Cytometry
Sample
Human Cell (peripheral immune cells)
Permeabilization
No
Gating Strategy
w work on purified cells. pDC were gated on BDCA2+HLADR+ and then
Specification
peripheral immune cells
Preparation
Cell harvesting/tissue preparation method: isolation of PBMC after ficoll plaque and purification of plasmacytoid dendritic cells
Sample buffer: cells were cultured in RPMI 10% SVF with the viral Ag at 4°C or 37°C and then wash before incubation with this anti-body.
Fixation
Paraformaldehyde
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Abcam user community

Verified customer

Submitted Oct 27 2016

Question

same as ab20930 but UNCONJUGATED.

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Answer

Thank you for your inquiry.

Please have follow this link for all our Hepatitis B Virus Surface Antigen (Ad/Ay) antibodies that are un-conjugated:

https://www.abcam.com/index.html?pageconfig=searchresults&search=Hepatitis B Virus Surface Antigen Ad Ay&pt=1&sk=targ&sv=Hepatitis B Virus Surface Antigen ad %2B ay&sn=Hepatitis B Virus Surface Antigen ad %2B ay&l=3&fViewMore=1

I will be happy to provide further help in choosing a suitable antibody when you provide more information about the samples, application etc you are planning to use.

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Question

I have been using this antibody in Flow Cytometry. I have sucessfully stained infected cells, however I am detecting positive signal in healthy control cells as well. I have tested multiple fixation methods as well as antibody dilutions of 1:10 - 1:100. I would like to try another lot of antibody.

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Answer

This is to confirm that a vial of ab20930 has been added free of charge to Cedarlane's order and will be dispatched today.

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Question

Dear Sir/Madam, May I enquire if there is any isotype control for the above mention antibody (AB20930) ? Many thanks for your assistance and hope to hear from you soon. Best Regards,

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Answer

Thank you for your enquiry. I can confirm that ab20930 Anti-Hepatitis B Virus Surface Antigen (Ad/Ay) antibody is a goat polyclonal antibody. Is is very unusual to have isotype controls for polyclonal antibodies, becuase they contain a mixture of isotypes. Therefore, regrettably we do not have a goat polyclonal isotype control available in the catalogue. I am sorry there are no suitable isotype controls to meet your requirements on this occasion. Should you have any further questions, please do not hesitate to contact us.

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