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    fitc-hepatitis-b-virus-surface-antigen-antibody-ab21021.pdf

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Microbiology Organism Virus DNA Virus double stranded DNA Virus Hepatitis B
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FITC Anti-Hepatitis B Virus Surface Antigen antibody (ab21021)

  • Datasheet
  • SDS
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Abpromise

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Key features and details

  • FITC Rabbit polyclonal to Hepatitis B Virus Surface Antigen
  • Suitable for: ICC/IF
  • Reacts with: Hepatitis B virus
  • Conjugation: FITC. Ex: 493nm, Em: 528nm
  • Isotype: IgG

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Overview

  • Product name

    FITC Anti-Hepatitis B Virus Surface Antigen antibody
    See all Hepatitis B Virus Surface Antigen primary antibodies
  • Description

    FITC Rabbit polyclonal to Hepatitis B Virus Surface Antigen
  • Host species

    Rabbit
  • Conjugation

    FITC. Ex: 493nm, Em: 528nm
  • Specificity

    Monospecific, reacts only with Hepatitis B surface antigen including the pre-S1 epitope. This product is non-reactive with normal human serum.
  • Tested applications

    Suitable for: ICC/IFmore details
  • Species reactivity

    Reacts with: Hepatitis B virus
  • Immunogen

    Hepatitis B surface antigen purified from human serum. Mixture of subtypes ad & ay.

  • General notes

    Covalently coupled with high purity Isomer I of fluorescein isothiocyanate. Care is taken to ensure complete removal of any free fluorescein from the final product.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.1% Sodium azide
    Constituents: 0.0268% PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Microbiology
    • Organism
    • Virus
    • DNA Virus
    • double stranded DNA Virus
    • Hepatitis B
    • Cancer
    • Oncoproteins/suppressors
    • Viral proteins
    • Hepatitis B

Associated products

  • Isotype control

    • FITC-Rabbit IgG - Isotype Control (ab37406)

Applications

Our Abpromise guarantee covers the use of ab21021 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
  • Application notes
    Direct FA staining: Use at an assay dependent dilution. A starting range of 1:10-1:50 is suggested. Acetone fixation of the antigen source is recommended prior to staining.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance

      Hepatitis B Virus (HBV) infection induces a disease state which manifests itself in a variety of ways, characterized by the extent of liver damage, inflammation and viral persistence. HBV infection is also associated with a 100 fold increased risk of hepatocellular carcinoma and currently infects over 250 million people worldwide. HBV has a partially double stranded 3.2 kilobase DNA genome which contains four open reading frames. One of these encodes a 154 amino acid protein called the HBx protein. HBx has been shown to be a transcriptional transactivator of both viral and cellular promoters. Lacking a DNA binding domain and nuclear localization signal, HBx is believed to exert transcriptional activity through protein protein interaction.
    • Cellular localization

      Virion membrane
    • Database links

      • Entrez Gene: 2703546 Hepatitis B virus
      • SwissProt: P03142 Hepatitis B virus
      • Alternative names

        • Large envelope protein antibody
        • Large surface protein antibody
        • LHB antibody
        • Major surface antigen antibody

      Protocols

      • Immunocytochemistry & immunofluorescence protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (1)

      Publishing research using ab21021? Please let us know so that we can cite the reference in this datasheet.

      ab21021 has been referenced in 1 publication.

      • Li G  et al. Recombinant covalently closed circular DNA of hepatitis B virus induces long-term viral persistence with chronic hepatitis in a mouse model. Hepatology 67:56-70 (2018). PubMed: 28749559

      Customer reviews and Q&As

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