FITC Anti-Hepatitis C Virus antibody [NS5B-6] (ab122979)

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Western blot - FITC Anti-Hepatitis C Virus antibody [NS5B-6] (ab122979)
  • Immunocytochemistry/ Immunofluorescence - FITC Anti-Hepatitis C Virus antibody [NS5B-6] (ab122979)

Key features and details

  • FITC Mouse monoclonal [NS5B-6] to Hepatitis C Virus
  • Suitable for: WB, ELISA, ICC/IF
  • Reacts with: Hepatitis C virus
  • Conjugation: FITC. Ex: 493nm, Em: 528nm
  • Isotype: IgG2b

Overview

  • Product name

    FITC Anti-Hepatitis C Virus antibody [NS5B-6]
    See all Hepatitis C Virus primary antibodies

  • Description

    FITC Mouse monoclonal [NS5B-6] to Hepatitis C Virus

  • Host species

    Mouse

  • Conjugation

    FITC. Ex: 493nm, Em: 528nm

  • Specificity

    Specific to Human HCV NS5b protein of genotype 1b. Not tested in other genotypes.

  • Tested applications

    Suitable for: WB, ELISA, ICC/IFmore details

  • Species reactivity

    Reacts with: Hepatitis C virus

  • Immunogen

    A region of NS5b protein of Hepatitis C Virus genotype 1b expressed in E. coli.

  • Positive control

    • Chimp liver cells and cell lysate, infected with recombinant vaccinia virus containing a HCV genome cDNA.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    pH: 6
    Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS
  • Concentration information loading...

  • Purity

    Proprietary Purification

  • Purification notes

    ab122979 is filter-sterilized. Purity >90% by SDS-PAGE.

  • Clonality

    Monoclonal

  • Clone number

    NS5B-6

  • Isotype

    IgG2b

  • Light chain type

    kappa

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab122979 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use at an assay dependent concentration. Predicted molecular weight: 327 kDa.
ELISA
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
Notes
WB
Use at an assay dependent concentration. Predicted molecular weight: 327 kDa.
ELISA
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.

Target

  • Relevance

    Hepatitis C Virus is a positive, single stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins and several non structural proteins necessary for viral replication. Several different genotypes of HCV with slightly different genomic sequences have since been identified that correlate with differences in response to treatment with interferon alpha.

  • Cellular localization

    Virus surface

  • Database links

    • Alternative names

      • Genome polyprotein antibody
      • HCV antibody
      • Hepatitis C virus polyprotein antibody

    Images

    • Western blot - FITC Anti-Hepatitis C Virus antibody [NS5B-6] (ab122979)
      Western blot - FITC Anti-Hepatitis C Virus antibody [NS5B-6] (ab122979)
      FITC Anti-Hepatitis C Virus antibody [NS5B-6] (ab122979) + Chimp liver cell lysate, infected with recombinant
      vaccinia virus containing a HCV genome cDNA

      Predicted band size: 327 kDa
      Observed band size: 58 kDa why is the actual band size different from the predicted?



      The NS5b protein is detected as a 58-kDa band.
    • Immunocytochemistry/ Immunofluorescence - FITC Anti-Hepatitis C Virus antibody [NS5B-6] (ab122979)
      Immunocytochemistry/ Immunofluorescence - FITC Anti-Hepatitis C Virus antibody [NS5B-6] (ab122979)
      ab122979, staining Hepatitis C Virus NS5b protein in Acetone-fixed Chimp liver cells infected with recombinant vaccinia virus containing a HCV genome cDNA by Immunofluorescence.

    References (1)

    Publishing research using ab122979? Please let us know so that we can cite the reference in this datasheet.

    ab122979 has been referenced in 1 publication.

    • Manabe S  et al. Production of nonstructural proteins of hepatitis C virus requires a putative viral protease encoded by NS3. Virology 198:636-44 (1994). PubMed: 8291245

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