Key features and details
- FITC Mouse monoclonal [NS5B-6] to Hepatitis C Virus
- Suitable for: WB, ELISA, ICC/IF
- Reacts with: Hepatitis C virus
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG2b
Product nameFITC Anti-Hepatitis C Virus antibody [NS5B-6]
See all Hepatitis C Virus primary antibodies
DescriptionFITC Mouse monoclonal [NS5B-6] to Hepatitis C Virus
ConjugationFITC. Ex: 493nm, Em: 528nm
SpecificitySpecific to Human HCV NS5b protein of genotype 1b. Not tested in other genotypes.
Tested applicationsSuitable for: WB, ELISA, ICC/IFmore details
Species reactivityReacts with: Hepatitis C virus
A region of NS5b protein of Hepatitis C Virus genotype 1b expressed in E. coli.
- Chimp liver cells and cell lysate, infected with recombinant vaccinia virus containing a HCV genome cDNA.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 6
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS
Concentration information loading...
Purification notesab122979 is filter-sterilized. Purity >90% by SDS-PAGE.
Light chain typekappa
Our Abpromise guarantee covers the use of ab122979 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 327 kDa.|
|ELISA||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
RelevanceHepatitis C Virus is a positive, single stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins and several non structural proteins necessary for viral replication. Several different genotypes of HCV with slightly different genomic sequences have since been identified that correlate with differences in response to treatment with interferon alpha.
Cellular localizationVirus surface
- Genome polyprotein antibody
- HCV antibody
- Hepatitis C virus polyprotein antibody
FITC Anti-Hepatitis C Virus antibody [NS5B-6] (ab122979) + Chimp liver cell lysate, infected with recombinant
vaccinia virus containing a HCV genome cDNA
Predicted band size: 327 kDa
Observed band size: 58 kDa why is the actual band size different from the predicted?
The NS5b protein is detected as a 58-kDa band.
ab122979, staining Hepatitis C Virus NS5b protein in Acetone-fixed Chimp liver cells infected with recombinant vaccinia virus containing a HCV genome cDNA by Immunofluorescence.
ab122979 has been referenced in 1 publication.
- Manabe S et al. Production of nonstructural proteins of hepatitis C virus requires a putative viral protease encoded by NS3. Virology 198:636-44 (1994). PubMed: 8291245