FITC Anti-Hepatitis C Virus antibody [NS5B-6] (ab122979)

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Western blot - FITC Anti-Hepatitis C Virus antibody [NS5B-6] (ab122979)
  • Immunocytochemistry/ Immunofluorescence - FITC Anti-Hepatitis C Virus antibody [NS5B-6] (ab122979)

Key features and details

  • FITC Mouse monoclonal [NS5B-6] to Hepatitis C Virus
  • Suitable for: WB, ELISA, ICC/IF
  • Reacts with: Hepatitis C virus
  • Conjugation: FITC. Ex: 493nm, Em: 528nm
  • Isotype: IgG2b

Overview

  • Product name

    FITC Anti-Hepatitis C Virus antibody [NS5B-6]
    See all Hepatitis C Virus primary antibodies

  • Description

    FITC Mouse monoclonal [NS5B-6] to Hepatitis C Virus

  • Host species

    Mouse

  • Conjugation

    FITC. Ex: 493nm, Em: 528nm

  • Specificity

    Specific to Human HCV NS5b protein of genotype 1b. Not tested in other genotypes.

  • Tested applications

    Suitable for: WB, ELISA, ICC/IFmore details

  • Species reactivity

    Reacts with: Hepatitis C virus

  • Immunogen

    A region of NS5b protein of Hepatitis C Virus genotype 1b expressed in E. coli.

  • Positive control

    • Chimp liver cells and cell lysate, infected with recombinant vaccinia virus containing a HCV genome cDNA.

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    pH: 6
    Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS
  • Concentration information loading...

  • Purity

    Proprietary Purification

  • Purification notes

    ab122979 is filter-sterilized. Purity >90% by SDS-PAGE.

  • Clonality

    Monoclonal

  • Clone number

    NS5B-6

  • Isotype

    IgG2b

  • Light chain type

    kappa

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab122979 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 327 kDa.
ELISA Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

Target

  • Relevance

    Hepatitis C Virus is a positive, single stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins and several non structural proteins necessary for viral replication. Several different genotypes of HCV with slightly different genomic sequences have since been identified that correlate with differences in response to treatment with interferon alpha.

  • Cellular localization

    Virus surface

  • Database links

    • Alternative names

      • Genome polyprotein antibody
      • HCV antibody
      • Hepatitis C virus polyprotein antibody

    Images

    • Western blot - FITC Anti-Hepatitis C Virus antibody [NS5B-6] (ab122979)
      Western blot - FITC Anti-Hepatitis C Virus antibody [NS5B-6] (ab122979)
      FITC Anti-Hepatitis C Virus antibody [NS5B-6] (ab122979) + Chimp liver cell lysate, infected with recombinant
      vaccinia virus containing a HCV genome cDNA

      Predicted band size: 327 kDa
      Observed band size: 58 kDa
      why is the actual band size different from the predicted?



      The NS5b protein is detected as a 58-kDa band.
    • Immunocytochemistry/ Immunofluorescence - FITC Anti-Hepatitis C Virus antibody [NS5B-6] (ab122979)
      Immunocytochemistry/ Immunofluorescence - FITC Anti-Hepatitis C Virus antibody [NS5B-6] (ab122979)
      ab122979, staining Hepatitis C Virus NS5b protein in Acetone-fixed Chimp liver cells infected with recombinant vaccinia virus containing a HCV genome cDNA by Immunofluorescence.

    References (1)

    Publishing research using ab122979? Please let us know so that we can cite the reference in this datasheet.

    ab122979 has been referenced in 1 publication.

    • Manabe S  et al. Production of nonstructural proteins of hepatitis C virus requires a putative viral protease encoded by NS3. Virology 198:636-44 (1994). PubMed: 8291245

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