FITC Anti-Hepatitis C Virus antibody [NS5B-6] (ab122979)
![Western blot - FITC Anti-Hepatitis C Virus antibody [NS5B-6] (ab122979)](https://www.abcam.com/ps/products/122/ab122979/Images/Hepatitis-C-Virus-Primary-antibodies-ab122979-3.jpg "Western blot - FITC Anti-Hepatitis C Virus antibody [NS5B-6] (ab122979)")
Key features and details
- FITC Mouse monoclonal [NS5B-6] to Hepatitis C Virus
- Suitable for: WB, ELISA, ICC/IF
- Reacts with: Hepatitis C virus
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG2b
Overview
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Product name
FITC Anti-Hepatitis C Virus antibody [NS5B-6]
See all Hepatitis C Virus primary antibodies -
Description
FITC Mouse monoclonal [NS5B-6] to Hepatitis C Virus -
Host species
Mouse -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Specificity
Specific to Human HCV NS5b protein of genotype 1b. Not tested in other genotypes. -
Tested applications
Suitable for: WB, ELISA, ICC/IFmore details -
Species reactivity
Reacts with: Hepatitis C virus -
Immunogen
A region of NS5b protein of Hepatitis C Virus genotype 1b expressed in E. coli.
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Positive control
- Chimp liver cells and cell lysate, infected with recombinant vaccinia virus containing a HCV genome cDNA.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS -
Concentration information loading... -
Purity
Proprietary Purification -
Purification notes
ab122979 is filter-sterilized. Purity >90% by SDS-PAGE. -
Clonality
Monoclonal -
Clone number
NS5B-6 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab122979 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use at an assay dependent concentration. Predicted molecular weight: 327 kDa.
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| ELISA |
Use at an assay dependent concentration.
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| ICC/IF |
Use at an assay dependent concentration.
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| Notes |
|---|
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WB
Use at an assay dependent concentration. Predicted molecular weight: 327 kDa. |
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ELISA
Use at an assay dependent concentration. |
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ICC/IF
Use at an assay dependent concentration. |
Target
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Relevance
Hepatitis C Virus is a positive, single stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins and several non structural proteins necessary for viral replication. Several different genotypes of HCV with slightly different genomic sequences have since been identified that correlate with differences in response to treatment with interferon alpha. -
Cellular localization
Virus surface -
Database links
- Entrez Gene: 951475 Hepatitis C virus
- SwissProt: P27958 Hepatitis C virus
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Alternative names
- Genome polyprotein antibody
- HCV antibody
- Hepatitis C virus polyprotein antibody
Images
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Western blot - FITC Anti-Hepatitis C Virus antibody [NS5B-6] (ab122979)FITC Anti-Hepatitis C Virus antibody [NS5B-6] (ab122979) + Chimp liver cell lysate, infected with recombinant
vaccinia virus containing a HCV genome cDNA
Predicted band size: 327 kDa
Observed band size: 58 kDa why is the actual band size different from the predicted?
The NS5b protein is detected as a 58-kDa band. -
![Immunocytochemistry/ Immunofluorescence - FITC Anti-Hepatitis C Virus antibody [NS5B-6] (ab122979)](https://www.abcam.com/ps/products/122/ab122979/Images/Hepatitis-C-Virus-Primary-antibodies-ab122979-4.jpg)
Immunocytochemistry/ Immunofluorescence - FITC Anti-Hepatitis C Virus antibody [NS5B-6] (ab122979)ab122979, staining Hepatitis C Virus NS5b protein in Acetone-fixed Chimp liver cells infected with recombinant vaccinia virus containing a HCV genome cDNA by Immunofluorescence.
Protocols
Datasheets and documents
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Datasheet download
References (1)
ab122979 has been referenced in 1 publication.
- Manabe S et al. Production of nonstructural proteins of hepatitis C virus requires a putative viral protease encoded by NS3. Virology 198:636-44 (1994). PubMed: 8291245
