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  1. Link

    fitc-hiv1-p24-antibody-ab30956.pdf

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Microbiology Organism Virus RNA Virus ssRNA positive strand virus HIV
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FITC Anti-HIV1 p24 antibody (ab30956)

  • Datasheet
  • SDS
Reviews (1)Q&A (1)

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Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Key features and details

  • FITC Goat polyclonal to HIV1 p24
  • Suitable for: WB, ICC
  • Reacts with: Species independent
  • Conjugation: FITC. Ex: 493nm, Em: 528nm
  • Isotype: IgG

You may also be interested in

Protein
Product image
Recombinant HIV1 p24 protein (ab43037)

View more associated products

Overview

  • Product name

    FITC Anti-HIV1 p24 antibody
    See all HIV1 p24 primary antibodies
  • Description

    FITC Goat polyclonal to HIV1 p24
  • Host species

    Goat
  • Conjugation

    FITC. Ex: 493nm, Em: 528nm
  • Tested applications

    Suitable for: WB, ICCmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Full length protein: HIV1 p24

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.1% Sodium azide
    Constituents: PBS, 0.1% BSA
  • Concentration information loading...
  • Purity

    Ion Exchange Chromatography
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Microbiology
    • Organism
    • Virus
    • RNA Virus
    • ssRNA positive strand virus
    • HIV
    • Immunology
    • Immune System Diseases
    • Antiviral Signaling
    • HIV

Associated products

  • Isotype control

    • FITC Goat IgG - Isotype control (ab37374)
  • Positive Controls

    • Recombinant HIV1 p24 protein (ab43037)
  • Recombinant Protein

    • Recombinant HIV1 p24 protein (ab43037)

Applications

Our Abpromise guarantee covers the use of ab30956 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 56 kDa.
ICC Use at an assay dependent concentration.

Target

  • Relevance

    HIV1 performs highly complex orchestrated tasks during the assembly, budding, maturation and infection stages of the viral replication cycle. During viral assembly, the proteins form membrane associations and self-associations that ultimately result in budding of an immature virion from the infected cell. Gag precursors also function during viral assembly to selectively bind and package two plus strands of genomic RNA. Capsid protein p24 probably forms the conical core of the virus that encapsulates the genomic RNA-nucleocapsid complex.
  • Cellular localization

    Membrane
  • Alternative names

    • CA antibody
    • Capsid protein p24 antibody
    • HIV-1 Gag p24 antibody
    • HIV1gp1 antibody
    • Human immunodeficiency virus 1 antibody
    • Human immunodeficiency virus type 1 p24 antibody
    see all

Protocols

  • Immunoprecipitation protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (0)

    Publishing research using ab30956? Please let us know so that we can cite the reference in this datasheet.

    ab30956 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-2 of 2 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for FITC Anti-HIV1 p24 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HEK 293T plus p24 (transfected))
    Permeabilization
    Yes - Triton X-100
    Specification
    HEK 293T plus p24 (transfected)
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 21°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 23 2020

    Question

    My customer wants to start with immunoprecipitation. He uses HIV proteins. He wants to buy your products Ab85536 , Ab30956 and Ab66641. But at first he has some questions:

    Ab85536- to what extent the clade B is specific? Is there any crossreactivity?

    Ab30956-to what extent is this antibody specific?

    Ab66641- to what extent the clade B is specific?



    One of these antibodies is conjugated with His-Taq. What is better?Immunoprecopitation or isolation over the colony?



    Do you have any special immunoprecipitation protocols?



    Do you have protein A and protein G, that are used in immunoprecipitation?



    Do you have some new products for immunoprecipitation for p55, glycoproteins gp41 and gp120?



    Thank you for your answer, I hope everything is clear.



    Best regards

    Read More

    Abcam community

    Verified customer

    Asked on Feb 22 2012

    Answer

    Thank you for contacting us.

    The answers to your questions are

    Ab85536- to what extent the clade B is specific? Is there any crossreactivity?

    A. We have note tested this antibody with HIV clade other than Clade B so unfortunately we are unable to share any data.

    Ab30956-to what extent is this antibody specific?

    A. We have note tested this antibody with HIV clade other than Clade B so unfortunately we are unable to share any data.

    Ab66641- to what extent the clade B is specific?

    A. We have note tested this antibody with HIV clade other than Clade B so unfortunately we are unable to share any data.

    One of these antibodies is conjugated with His-Taq. What is better?Immunoprecopitation or isolation over the colony?

    A. The immunogen of ab85536 is HIs tagged not the antibody. It will not affect the use of antibody in IP. You can certainly used this ab in IP.

    Do you have any special immunoprecipitation protocols?

    A. Customised protocols are not required for these product so lease try the standard protocols.
    https://www.abcam.com/index.html?pageconfig=popular_protocols

    Do you have protein A and protein G, that are used in immunoprecipitation?

    A. We have protein A ab7399, and protein G, ab73758 as proteins. These products are not conjugated to any bead. You may need to get protein A or protein G conjugated beads for IP. Please check the IP protocol for more info.

    Do you have some new products for immunoprecipitation for p55, glycoproteins gp41 and gp120

    We do not have anti p55, gp41 and gp120 IP tested antibodies. However this does not mean these can't be used. If you are interested in using these in IP then let me know you may be eligible for Abreview testing discount. Please click the link for more info;

    https://www.abcam.com/index.html?pageconfig=resource&rid=11998&viapagetrap=collaborationdiscount

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on Feb 22 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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