FITC Anti-HLA A2 antibody [BB7.2] (ab27728)

Submit a review Q&A (2)References (8)
Flow Cytometry - FITC Anti-HLA A2 antibody [BB7.2] (ab27728)

    Key features and details

    • FITC Mouse monoclonal [BB7.2] to HLA A2
    • Suitable for: Flow Cyt
    • Reacts with: Human
    • Conjugation: FITC. Ex: 493nm, Em: 528nm
    • Isotype: IgG2b

    Overview

    • Product name

      FITC Anti-HLA A2 antibody [BB7.2]
      See all HLA A2 primary antibodies

    • Description

      FITC Mouse monoclonal [BB7.2] to HLA A2

    • Host species

      Mouse

    • Conjugation

      FITC. Ex: 493nm, Em: 528nm

    • Specificity

      Ab27728 recognises the human HLA A2 histocompatability antigen.

    • Tested applications

      Suitable for: Flow Cytmore details

    • Species reactivity

      Reacts with: Human

    • Immunogen

      Papain solubilised HLA A2 (Human).

    • Epitope

      The epitope recognised by this antibody has been studied extensively and would appear to include the carboxy terminus of the alpha 2 helix and a turn on one of the underlying beta strands.

    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Store at +4°C.

    • Storage buffer

      Preservative: 0.09% Sodium azide
      Constituent: 1% BSA
    • Concentration information loading...

    • Purity

      Protein G purified

    • Purification notes

      Ab27728 was purified from tissue culture supernatant, and prepared by affinity chromatography.

    • Clonality

      Monoclonal

    • Clone number

      BB7.2

    • Myeloma

      NS1

    • Isotype

      IgG2b

    • Research areas

    Applications

    Our Abpromise guarantee covers the use of ab27728 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt 1/10.

    Use 10 µl of the suggested working dilution to label 106 cells or 100µl whole blood.

     

     

     

    ab91368 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

     

    Target

    • Relevance

      Involved in the presentation of foreign antigens to the immune system. Dimer of alpha chain and a beta chain (beta-2-microglobulin). Interacts with human herpesvirus 8 MIR1 protein.

    • Cellular localization

      Membrane; Single-pass type I membrane protein.

    • Database links

      • Alternative names

        • Antigen presenting molecule antibody
        • HLA A antibody
        • HLA A histocompatibility type antibody
        • HLA antibody
        • HLA class I antibody
        • HLA class I histocompatibility antigen A 2 alpha chain antibody
        • HLA class I histocompatibility antigen A2 alpha chain antibody
        • HLA-A antibody
        • HLAA antibody
        • Human leukocyte antigen antibody
        • Leucocyte antigen class I antibody
        • Leukocyte antigen class I A antibody
        • Major histocompatibility complex class I A antibody
        • Major histocompatibility complex class I antibody
        • MHC class I antigen [precursor] antibody
        • MHC class I antigen A antibody
        • MHC class I antigen A*2 antibody
        • MHC class I antigen A2 antibody
        • MHC class I antigen HLA A antibody
        • MHC class I antigen HLA A heavy chain antibody
        • MHC leukocyte antigen antibody
        see all

      Images

      • Flow Cytometry - FITC Anti-HLA A2 antibody [BB7.2] (ab27728)
        Flow Cytometry - FITC Anti-HLA A2 antibody [BB7.2] (ab27728)
        Ab27728, undiluted or at a dilution of 1/10, staining HLA A2 in human peripheral blood monocytes by FACS.

      References (8)

      Publishing research using ab27728? Please let us know so that we can cite the reference in this datasheet.

      ab27728 has been referenced in 8 publications.

      • Viborg N  et al. T cell recognition of novel shared breast cancer antigens is frequently observed in peripheral blood of breast cancer patients. Oncoimmunology 8:e1663107 (2019). PubMed: 31741759
      • Li D  et al. Engineering chimeric human and mouse major histocompatibility complex (MHC) class I tetramers for the production of T-cell receptor (TCR) mimic antibodies. PLoS One 12:e0176642 (2017). Flow Cyt . PubMed: 28448627
      • Kim YH  et al. Branched multipeptide immunotherapy for glioblastoma using human leukocyte antigen-A*0201-restricted cytotoxic T-lymphocyte epitopes from ERBB2, BIRC5 and CD99. Oncotarget 7:50535-50547 (2016). PubMed: 27409668
      • Straetemans T  et al. Recurrence of melanoma following T cell treatment: continued antigen expression in a tumor that evades T cell recruitment. Mol Ther 23:396-406 (2015). PubMed: 25363716
      • Cheent KS  et al. Synergistic inhibition of natural killer cells by the nonsignaling molecule CD94. Proc Natl Acad Sci U S A 110:16981-6 (2013). Human . PubMed: 24082146
      • Whitfield-Larry F  et al. HLA-A2-matched peripheral blood mononuclear cells from type 1 diabetic patients, but not nondiabetic donors, transfer insulitis to NOD-scid/?c(null)/HLA-A2 transgenic mice concurrent with the expansion of islet-specific CD8+ T cells. Diabetes 60:1726-33 (2011). Flow Cyt . PubMed: 21521873
      • Hogan KT & Brown SL Localization and characterization of serologic epitopes on HLA-A2. Hum Immunol 33:185-92 (1992). PubMed: 1377666
      • Parham P & Brodsky FM Partial purification and some properties of BB7.2. A cytotoxic monoclonal antibody with specificity for HLA-A2 and a variant of HLA-A28. Hum Immunol 3:277-99 (1981). PubMed: 7035415

      Customer reviews and Q&As

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      1-2 of 2 Abreviews or Q&A

      Question

      The Ab reported into this paper is not ab74674 but the FITC conjugated ab27728.
      Kind regards

      Read More
      Answer

      Thank you for contacting us.

      Unfortunately we have not determined which specific HLA-A2 allele(s) this clone binds. However, it is clear from the paper you kindly provided that the clone binds HLA-A201. Unfortunately, we currently do not have any empirical data to indicate if this clone is specific just to the HLA-A201 allele or if detects other alleles.

      http://www.ncbi.nlm.nih.gov/pubmed/19265143

      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Use our products? Submit an Abreview. Earn rewards!
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      Question

      BATCH NUMBER 217168 ORDER NUMBER 168928 DESCRIPTION OF THE PROBLEM No signal. SAMPLE (Human) SupT1 cells (Human) Jurkat T cells PRIMARY ANTIBODY AbCam: Mouse monoclonal [BB7.2] to HLA A2(FITC). Used neat at 10uL per million cells Incubated for 1hr on ice or 1hr at Room Temp. Washed either once, twice or three times with 1xPBS DETECTION METHOD FACS POSITIVE AND NEGATIVE CONTROLS USED Negative Controls: Used unstained cells from both cell lines. Positive Controls: Used MHC-APC antibody* Initially used MHC-APC antibody*, but was hoping to use the AbCam to isolate specifically the HLA A2 population. ANTIBODY STORAGE CONDITIONS Ab received at room temp. Kept at 4C for ~2.5 weeks (while being tested) remainder was aliquoted and kept at -20C. SAMPLE PREPARATION Both cell lines are grown in 10% RPMI Ab/Amyc. Cells were resuspended in either solution buffers prior to Ab staining: 1xPBS or 1xPBS + 2%FBS NUMBER OF CELLS USED 0.5x10^6 (500,000) 1.0x10^6 (1,000,000) PERMEABILIZATION STEP N/A BLOCKING CONDITIONS Either not blocked (1xPBS) or blocked (1xPBS + 2%FBS) ~20min SECONDARY ANTIBODY N/A HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 4 HAVE YOU RUN A "NO PRIMARY" CONTROL? Yes DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes WHAT STEPS HAVE YOU ALTERED? Altered primary AbCam conjugated Ab incubation times (45 min up to 1.25 hours), whether on ice or not, and whether (or not) blocking (FBS) solution was included.

      Read More
      Answer

      Thank you for filling out the questionnaire. Your protocol looks good. Do you incubate the cells with the antibody in the dark? FITC is sensitive to photo-bleaching. The only other recommendation I can make would be to try BSA instead of FBS for blocking, in case there are interactions with fetal serum proteins other than BSA that are blocking binding of the antibody to the HLA A2. The PBS-only control is a good idea but you may be losing the antibody to hydrophobic interactions with the tube you incubate in when you leave out blocking protein. The BSA is admittedly a long-shot solution. If you feel photo-bleaching is not a problem, then I can send you a free-of-charge replacement vial, or a refund or credit. Let me know what you would like.

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