FITC Anti-HLA G antibody [87G] (ab239336)

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Flow Cytometry - FITC Anti-HLA G antibody [87G] (ab239336)

    Key features and details

    • FITC Mouse monoclonal [87G] to HLA G
    • Suitable for: Flow Cyt
    • Reacts with: Human
    • Conjugation: FITC. Ex: 493nm, Em: 528nm
    • Isotype: IgG2a

    Overview

    • Product name

      FITC Anti-HLA G antibody [87G]
      See all HLA G primary antibodies

    • Description

      FITC Mouse monoclonal [87G] to HLA G

    • Host species

      Mouse

    • Conjugation

      FITC. Ex: 493nm, Em: 528nm

    • Specificity

      The antibody 87G recognizes both membrane-bound and soluble forms of HLA-G (HLA-G1 and HLA-G5).

    • Tested applications

      Suitable for: Flow Cytmore details

    • Species reactivity

      Reacts with: Human
      Does not react with: Mouse, Rat

    • Immunogen

      Tissue, cells or virus corresponding to Human HLA G. (HLA-B27 transgenic mice were imunized with H-2 identical murine cells transfected with and expressing genes encoding HLA-G and human beta2-microglobulin).

    • Positive control

      • Flow Cyt: HLA-G transfectants.

    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Store at +4°C. Store In the Dark.

    • Storage buffer

      pH: 7.40
      Preservative: 0.0975% Sodium azide
      Constituent: PBS
    • Concentration information loading...

    • Purity

      Size exclusion

    • Purification notes

      Purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC.

    • Clonality

      Monoclonal

    • Clone number

      87G

    • Isotype

      IgG2a

    • Research areas

    Applications

    Our Abpromise guarantee covers the use of ab239336 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt 1/100.

    Target

    • Function

      Involved in the presentation of foreign antigens to the immune system. Plays a role in maternal tolerance of the fetus by mediating protection from the deleterious effects of natural killer cells, cytotoxic T lymphocytes, macrophages and mononuclear cells.

    • Tissue specificity

      Expressed in trophoblasts.

    • Sequence similarities

      Belongs to the MHC class I family.
      Contains 1 Ig-like C1-type (immunoglobulin-like) domain.

    • Cellular localization

      Membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • B2 microglobulin antibody
        • DADB-15K14.8 antibody
        • HLA 6.0 antibody
        • HLA class I histocompatibility antigen alpha chain G antibody
        • HLA class I histocompatibility antigen, alpha chain G antibody
        • HLA class I molecule antibody
        • HLA G antibody
        • HLA G antigen antibody
        • HLA G histocompatibility antigen class I G antibody
        • HLA G3 antibody
        • HLA-G antibody
        • HLA-G histocompatibility antigen, class I antibody
        • HLA60 antibody
        • HLAG antibody
        • HLAG_HUMAN antibody
        • Major histocompatibility complex class I G antibody
        • MHC class I antigen antibody
        • MHC class I antigen G antibody
        • MHC G antibody
        • T-cell A locus antibody
        • TCA antibody
        see all

      Images

      • Flow Cytometry - FITC Anti-HLA G antibody [87G] (ab239336)
        Flow Cytometry - FITC Anti-HLA G antibody [87G] (ab239336)

        Flow cytometric analysis of HLA-G transfectants labeling HLA-G with ab239336 (red), compared with a control (blue). Surface staining. Gated on cell singlets.

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      References (0)

      Publishing research using ab239336? Please let us know so that we can cite the reference in this datasheet.

      ab239336 has not yet been referenced specifically in any publications.

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