FITC Anti-Hsp70 antibody [C92F3A-5] (ab61907)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FITC Anti-Hsp70 antibody [C92F3A-5] (ab61907)](https://www.abcam.com/ps/products/61/ab61907/Images/ab61907-449256-ab4745559915antihsp70antibodyc92f3a5immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FITC Anti-Hsp70 antibody [C92F3A-5] (ab61907)")
Key features and details
- FITC Mouse monoclonal [C92F3A-5] to Hsp70
- Suitable for: Flow Cyt, IHC-P
- Reacts with: Mouse, Rat, Sheep, Rabbit, Chicken, Guinea pig, Hamster, Cow, Dog, Human, Pig, Carp, Drosophila melanogaster, Monkey
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG
Overview
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Product name
FITC Anti-Hsp70 antibody [C92F3A-5]
See all Hsp70 primary antibodies -
Description
FITC Mouse monoclonal [C92F3A-5] to Hsp70 -
Host species
Mouse -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Specificity
ab61907 detects a 70kDa protein corresponding to the molecular mass of inducible hsp70 on SDS PAGE immunoblots. There is no cross-reactivity to hsc70 (hsp73). -
Tested applications
Suitable for: Flow Cyt, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Sheep, Rabbit, Chicken, Guinea pig, Hamster, Cow, Dog, Human, Pig, Carp, Drosophila melanogaster, Monkey -
Immunogen
Full length Hsp70 protein (Human)
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Positive control
- IHC-P: Mouse colon cancer tissue; human colon cancer tissue. Flow cyt: Heat Shocked CD3+ CD8+ T cells
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
C92F3A-5 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab61907 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | 1/1000. | |
| IHC-P | 1/10000. |
Target
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Relevance
Function: In cooperation with other chaperones, the Hsp70 family stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. In case of rotavirus A infection, serves as a post-attachment receptor for the virus to facilitate entry into the cell. Tissue specificity: HSPA1B is testis-specific. -
Cellular localization
Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. -
Database links
- Entrez Gene: 281825 Cow
- Entrez Gene: 403612 Dog
- Entrez Gene: 15511 Human
- Entrez Gene: 3303 Human
- Entrez Gene: 193740 Mouse
- Entrez Gene: 3304 Mouse
- Entrez Gene: 396906 Pig
- Entrez Gene: 24472 Rat
see all -
Alternative names
- DnaK type molecular chaperone HSP70 1 antibody
- Epididymis secretory protein Li 103 antibody
- FLJ54303 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FITC Anti-Hsp70 antibody [C92F3A-5] (ab61907)
ab61907 staining Hsp70 in mouse colon cancer tissue section by Immunohistochemistry (Bouin's fixed paraffin embedded tissue sections). The primary antibody was diluted at 1/100,000. A Fluorophore conjugated goat anti mouse was used as secondary. An antibody amplifier™ system was used for staining.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FITC Anti-Hsp70 antibody [C92F3A-5] (ab61907)](https://www.abcam.com/ps/products/61/ab61907/Images/ab61907-449255-ab61907308183antihsp70antibodyc92f3a5immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FITC Anti-Hsp70 antibody [C92F3A-5] (ab61907)Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for Hsp70 using ab61907 at 1/1000 dilution in immunohistochemical analysis. Counter stained with hematoxylin.
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![Flow Cytometry - FITC Anti-Hsp70 antibody [C92F3A-5] (ab61907)](https://www.abcam.com/ps/products/61/ab61907/Images/ab61907-199650-anti-hsp70-antibody-c92f3a-5-fitc-flow-cytometry.jpg)
Flow Cytometry - FITC Anti-Hsp70 antibody [C92F3A-5] (ab61907)FACS analysis using ab61907 staining heat shock treated CD3 + CD8 + T cells.
Datasheets and documents
References (3)
ab61907 has been referenced in 3 publications.
- Zhou B et al. Assisting anti-PD-1 antibody treatment with a liposomal system capable of recruiting immune cells. Nanoscale 11:7996-8011 (2019). PubMed: 30969294
- Manukyan G et al. Accelerated apoptosis of neutrophils in familial mediterranean Fever. Front Immunol 6:239 (2015). PubMed: 26042122
- Weiss LM et al. Bradyzoite development in Toxoplasma gondii and the hsp70 stress response. Infect Immun 66:3295-302 (1998). PubMed: 9632598
