FITC Anti-Human IgM Fc fragment antibody [CH2] (ab239227)
Key features and details
- FITC Mouse monoclonal [CH2] to Human IgM Fc fragment
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG1
Overview
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Product name
FITC Anti-Human IgM Fc fragment antibody [CH2] -
Description
FITC Mouse monoclonal [CH2] to Human IgM Fc fragment -
Host species
Mouse -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Specificity
The antibody CH2 reacts with Fc fragment of human IgM. -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length native protein (purified) corresponding to Human IgM Fc fragment. Purified.
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Positive control
- Flow Cyt: Human peripheral blood cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.0975% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Size exclusion -
Purification notes
The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC. -
Clonality
Monoclonal -
Clone number
CH2 -
Isotype
IgG1 -
Research areas
Associated products
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab239227 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt |
Use a concentration of 1 µg/ml.
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Notes |
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Flow Cyt
Use a concentration of 1 µg/ml. |
Target
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Relevance
Immunoglobulin M (IgM) is produced as a 900 kDa pentamer, which is an efficient complement binder. This antibody type is produced initially in the immune response and it is the first immunoglobulin class to be synthesized by a fetus or newborn. IgM antibodies do not cross the placenta. IgM concentration in blood is 0.12 g/l and its biological survival (plasma T1/2) is 5 days. -
Cellular localization
Cell Membrane and Secreted -
Database links
- Entrez Gene: 3507 Human
- Omim: 147020 Human
- SwissProt: P01871 Human
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Alternative names
- DKFZp686I15196 antibody
- DKFZp686I15212 antibody
- FLJ00385 antibody
see all
Images
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Flow cytometry anaysis of human peripheral whole blood labeling human IgM Fc fragment with ab239227 at 1 μg/mL.
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Flow cytometry anaysis of human peripheral whole blood labeling human IgM Fc fragment with ab239227 at 1 μg/mL.
CD19 labeled using anti-human CD19 (LT19) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).
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Flow cytometry anaysis of human peripheral whole blood labeling human IgM Fc fragment with ab239227 at 1 μg/mL.
Histogram shows separation of human IgM positive CD19 positive B-cells (red-filled) from IgM negative CD19 negative lymphocytes (black-dashed).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab239227 has not yet been referenced specifically in any publications.