For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    fitc-iba1-antibody-1022-5-ab15691.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Cell Biology Cell Cycle Cell Cycle Inhibitors Other
Share by email

FITC Anti-Iba1 antibody [1022-5] (ab15691)

  • Datasheet
  • SDS
Reviews (2)Q&A (2)References (6)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Flow Cytometry - FITC Anti-Iba1 antibody [1022-5] (ab15691)

    Key features and details

    • FITC Mouse monoclonal [1022-5] to Iba1
    • Suitable for: Flow Cyt
    • Reacts with: Rat, Human
    • Conjugation: FITC. Ex: 493nm, Em: 528nm
    • Isotype: IgG2b

    You may also be interested in

    Conjugation
    Product image
    FITC Conjugation Kit - Lightning-Link® (ab102884)
    Protein
    Product image
    Recombinant Human Iba1 protein (ab105593)
    Primary
    Product image
    Anti-TMEM119 antibody - C-terminal (ab185333)

    View more associated products

    Overview

    • Product name

      FITC Anti-Iba1 antibody [1022-5]
      See all Iba1 primary antibodies
    • Description

      FITC Mouse monoclonal [1022-5] to Iba1
    • Host species

      Mouse
    • Conjugation

      FITC. Ex: 493nm, Em: 528nm
    • Tested applications

      Suitable for: Flow Cytmore details
      Unsuitable for: IHC
    • Species reactivity

      Reacts with: Rat, Human
    • Immunogen

      Recombinant full length protein corresponding to Human Iba1.
      Database link: P55008

    • Positive control

      • Flow Cytometry: THP-1 cells.
    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C.
    • Storage buffer

      pH: 7.20
      Preservative: 0.1% Sodium azide
      Constituents: PBS, 1% BSA
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Monoclonal
    • Clone number

      1022-5
    • Isotype

      IgG2b
    • Research areas

      • Cell Biology
      • Cell Cycle
      • Cell Cycle Inhibitors
      • Other
      • Immunology
      • Immune Interventions
      • Transplantation
      • Immunology
      • Innate Immunity
      • Macrophage / Inflamm.
      • Neuroscience
      • Cell Type Marker
      • Glia marker
      • Microglia marker
      • Cell Biology
      • Other Antibodies
      • Oxidative Stress
      • Metabolism
      • Pathways and Processes
      • Redox metabolism
      • Oxidative stress

    Associated products

    • Alternative Versions

      • Anti-Iba1 antibody [1022-5] (ab15690)
    • Isotype control

      • FITC Mouse IgG2b [PLPV219] - Isotype Control (ab91368)
    • Recombinant Protein

      • Recombinant Human Iba1 protein (ab105593)

    Applications

    Our Abpromise guarantee covers the use of ab15691 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt Use 10µl for 106 cells.

    ab91368 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

     

  • Application notes
    Is unsuitable for IHC.
  • Target

    • Function

      Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation.
    • Tissue specificity

      Detected in T-lymphocytes and peripheral blood mononuclear cells.
    • Sequence similarities

      Contains 2 EF-hand domains.
    • Post-translational
      modifications

      Phosphorylated on serine residues.
    • Cellular localization

      Cytoplasm > cytoskeleton. Cell projection > ruffle membrane. Associated with the actin cytoskeleton at membrane ruffles and at sites of phagocytosis.
    • Target information above from: UniProt accession P55008 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 199 Human
      • Entrez Gene: 29427 Rat
      • Omim: 601833 Human
      • SwissProt: P55008 Human
      • SwissProt: P55009 Rat
      • Unigene: 76364 Human
      • Unigene: 32080 Rat
      • Alternative names

        • AIF 1 antibody
        • AIF-1 antibody
        • Aif1 antibody
        • AIF1 protein antibody
        • AIF1_HUMAN antibody
        • Allograft inflammatory factor 1 antibody
        • Allograft inflammatory factor 1 splice variant G antibody
        • allograft inflammatory factor-1 splice variant Hara-1 antibody
        • balloon angioplasty responsive transcription antibody
        • BART 1 antibody
        • G1 antibody
        • G1 putative splice variant of allograft inflamatory factor 1 antibody
        • IBA 1 antibody
        • IBA1 antibody
        • interferon gamma responsive transcript antibody
        • Interferon responsive transcript 1 antibody
        • interferon responsive transcript factor 1 antibody
        • Ionized calcium binding adapter molecule 1 antibody
        • Ionized calcium-binding adapter molecule 1 antibody
        • ionized calcium-binding adapter molecule antibody
        • IRT 1 antibody
        • IRT1 antibody
        • Microglia response factor antibody
        • MRF1 antibody
        • Protein g1 antibody
        see all

      Images

      • Flow Cytometry - FITC Anti-Iba1 antibody [1022-5] (ab15691)
        Flow Cytometry - FITC Anti-Iba1 antibody [1022-5] (ab15691)

        Overlay histogram showing THP-1 (human monocytic leukemia cell line) cells stained with ab15691 (red line). The cells were fixed with 80% methanol (5 minutes) and then permeabilized with 0.1% PBS-Tween for 20 minutes. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab15691, 10 µl/1 x 106 cells) for 30 minutes at 22ºC. Isotype control antibody (black line) was mouse IgG2b (FITC) (ab91368, 10 µl/1 x 106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in THP-1 cells fixed with 4% paraformaldehyde (10 minutes)/permeabilized in 0.1% PBS-Tween used under the same conditions.

      Protocols

      • Flow cytometry protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (6)

      Publishing research using ab15691? Please let us know so that we can cite the reference in this datasheet.

      ab15691 has been referenced in 6 publications.

      • Trkov Bobnar S  et al. Fingolimod Suppresses the Proinflammatory Status of Interferon-?-Activated Cultured Rat Astrocytes. Mol Neurobiol N/A:N/A (2019). PubMed: 30701416
      • Lei J  et al. Maternal dendrimer-based therapy for inflammation-induced preterm birth and perinatal brain injury. Sci Rep 7:6106 (2017). Flow Cyt ; Mouse . PubMed: 28733619
      • Li C  et al. Inhibitory Effects of Scolopendra Pharmacopuncture on the Development and Maintenance of Neuropathic Pain in Rats: Possible Involvement of Spinal Glial Cells. J Acupunct Meridian Stud 8:236-44 (2015). PubMed: 26433800
      • Tucsek Z  et al. Obesity in Aging Exacerbates Blood-Brain Barrier Disruption, Neuroinflammation, and Oxidative Stress in the Mouse Hippocampus: Effects on Expression of Genes Involved in Beta-Amyloid Generation and Alzheimer's Disease. J Gerontol A Biol Sci Med Sci : (2013). PubMed: 24269929
      • Schluesener HJ  et al. Effects of autoantigen and dexamethasone treatment on expression of endothelial-monocyte activating polypeptide II and allograft-inflammatory factor-1 by activated macrophages and microglial cells in lesions of experimental autoimmune encephalomyelitis, neuritis and uveitis. Acta Neuropathol (Berl) 97:119-26 (1999). PubMed: 9928822
      • Schluesener HJ  et al. Allograft-inflammatory factor-1 in rat experimental autoimmune encephalomyelitis, neuritis, and uveitis: expression by activated macrophages and microglial cells. Glia 24:244-51 (1998). PubMed: 9728770

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-4 of 4 Abreviews or Q&A

      Question

      How much is the 1mg PE-conjugating-ab15690?

      Read More

      Abcam community

      Verified customer

      Asked on Feb 14 2012

      Answer

      I have spoken to our custom order specialist and they have told me that if you would like 1mg of ab15690 conjugated to PE, then the cost would be over $3000.00. If you would like to go ahead and place a custom order for ab15690, please let me know and I will pass your information onto our in house specialist, who will complete the order for you.

      I look forward to your reply.

      Read More

      Abcam Scientific Support

      Answered on Feb 14 2012

      Question

      Thank you very much for your response.

      I prefer to the conjugates for the excitation wavelength of 488nm and emission wavelength of 575nm. The conjugates you suggested might not work for my machine.

      Do you have another conjugation available?

      How much is the 1mg PE-conjugating-ab15690?

      Read More

      Abcam community

      Verified customer

      Asked on Feb 14 2012

      Answer

      Thank you for your reply.

      I have forward your request about the quote for 1mg of ab15690 conjugated to PE to our in house specialist and I will let you know as soon as I hear back from them.

      As to other conjugates that would work for your machine, you can find a complete list of our EasyLink kits in the link below:

      https://www.abcam.com/easylink



      Please let me know if there is anything else I can help you with and I will get back to you as soon as possible about ab15960.

      Read More

      Abcam Scientific Support

      Answered on Feb 14 2012

      Flow Cytometry abreview for Anti-Iba1 antibody [1022-5] (FITC) - Microglial marker

      Average
      Abreviews
      Abreviews
      abreview image
      Application
      Flow Cytometry
      Sample
      Mouse Cell (Brain)
      Specification
      Brain
      Fixation
      Paraformaldehyde
      Permeabilization
      Yes - BD Perm/Wash
      Gating Strategy
      Microglia
      Read More

      Abcam user community

      Verified customer

      Submitted Feb 01 2012

      Flow Cytometry abreview for Anti-Iba1 antibody [1022-5] (FITC) - Microglial marker

      Excellent
      Abreviews
      Abreviews
      Application
      Flow Cytometry
      Sample
      Mouse Cell (spinal cord neuron)
      Specification
      spinal cord neuron
      Preparation
      Cell harvesting/tissue preparation method: primary cell culture
      Sample buffer: 0.5% BSA + 2 mM EDTA/PBS without divalent cations
      Fixation
      Paraformaldehyde
      Permeabilization
      No
      Gating Strategy
      macrophages
      Read More

      Ms. Minjung Kwon

      Verified customer

      Submitted Jan 21 2010

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

      Get resources and offers direct to your inbox Sign up
      A-Z by research area
      • Cancer
      • Cardiovascular
      • Cell biology
      • Developmental biology
      • Epigenetics & Nuclear signaling
      • Immunology
      • Metabolism
      • Microbiology
      • Neuroscience
      • Signal transduction
      • Stem cells
      A-Z by product type
      • Primary antibodies
      • Secondary antibodies
      • Biochemicals
      • Isotype controls
      • Flow cytometry multi-color selector
      • Kits
      • Loading controls
      • Lysates
      • Peptides
      • Proteins
      • Slides
      • Tags and cell markers
      • Tools & Reagents
      Help & support
      • Support
      • Make an Inquiry
      • Protocols & troubleshooting
      • Placing an order
      • RabMAb products
      • Biochemical product FAQs
      • Training
      • Browse by Target
      Company
      • Corporate site
      • Investor relations
      • Company news
      • Careers
      • About us
      • Blog
      Events
      • Tradeshows
      • Conferences
      International websites
      • abcam.cn
      • abcam.co.jp

      Join with us

      • LinkedIn
      • facebook
      • Twitter
      • YouTube
      • Terms of sale
      • Website terms of use
      • Cookie policy
      • Privacy policy
      • Legal
      • Modern slavery statement
      © 1998-2021 Abcam plc. All rights reserved.