FITC Anti-Iba1 antibody [1022-5] (ab15691)
![Flow Cytometry - FITC Anti-Iba1 antibody [1022-5] (ab15691)](https://www.abcam.com/ps/products/15/ab15691/Images/ab15691-23327-anti-iba1-antibody-1022-5-fitc-flow-cytometry.jpg "Flow Cytometry - FITC Anti-Iba1 antibody [1022-5] (ab15691)")
Key features and details
- FITC Mouse monoclonal [1022-5] to Iba1
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG2b
Overview
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Product name
FITC Anti-Iba1 antibody [1022-5]
See all Iba1 primary antibodies -
Description
FITC Mouse monoclonal [1022-5] to Iba1 -
Host species
Mouse -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Tested applications
Suitable for: Flow Cytmore details
Unsuitable for: IHC -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human Iba1.
Database link: P55008 -
Positive control
- Flow Cytometry: THP-1 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.20
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Monoclonal -
Clone number
1022-5 -
Isotype
IgG2b -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab15691 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | (2) |
Use 10µl for 106 cells.
ab91368 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
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| Notes |
|---|
|
Flow Cyt
Use 10µl for 106 cells. ab91368 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
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Target
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Function
Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation. -
Tissue specificity
Detected in T-lymphocytes and peripheral blood mononuclear cells. -
Sequence similarities
Contains 2 EF-hand domains. -
Post-translational
modificationsPhosphorylated on serine residues. -
Cellular localization
Cytoplasm > cytoskeleton. Cell projection > ruffle membrane. Associated with the actin cytoskeleton at membrane ruffles and at sites of phagocytosis. - Information by UniProt
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Database links
- Entrez Gene: 199 Human
- Omim: 601833 Human
- SwissProt: P55008 Human
- Unigene: 76364 Human
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Alternative names
- AIF 1 antibody
- AIF-1 antibody
- Aif1 antibody
see all
Images
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Flow Cytometry - FITC Anti-Iba1 antibody [1022-5] (ab15691)
Overlay histogram showing THP-1 (human monocytic leukemia cell line) cells stained with ab15691 (red line). The cells were fixed with 80% methanol (5 minutes) and then permeabilized with 0.1% PBS-Tween for 20 minutes. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab15691, 10 µl/1 x 106 cells) for 30 minutes at 22ºC. Isotype control antibody (black line) was mouse IgG2b (FITC) (ab91368, 10 µl/1 x 106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in THP-1 cells fixed with 4% paraformaldehyde (10 minutes)/permeabilized in 0.1% PBS-Tween used under the same conditions.
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab15691 has been referenced in 6 publications.
- Trkov Bobnar S et al. Fingolimod Suppresses the Proinflammatory Status of Interferon-?-Activated Cultured Rat Astrocytes. Mol Neurobiol N/A:N/A (2019). PubMed: 30701416
- Lei J et al. Maternal dendrimer-based therapy for inflammation-induced preterm birth and perinatal brain injury. Sci Rep 7:6106 (2017). Flow Cyt ; Mouse . PubMed: 28733619
- Li C et al. Inhibitory Effects of Scolopendra Pharmacopuncture on the Development and Maintenance of Neuropathic Pain in Rats: Possible Involvement of Spinal Glial Cells. J Acupunct Meridian Stud 8:236-44 (2015). PubMed: 26433800
- Tucsek Z et al. Obesity in Aging Exacerbates Blood-Brain Barrier Disruption, Neuroinflammation, and Oxidative Stress in the Mouse Hippocampus: Effects on Expression of Genes Involved in Beta-Amyloid Generation and Alzheimer's Disease. J Gerontol A Biol Sci Med Sci : (2013). PubMed: 24269929
- Schluesener HJ et al. Effects of autoantigen and dexamethasone treatment on expression of endothelial-monocyte activating polypeptide II and allograft-inflammatory factor-1 by activated macrophages and microglial cells in lesions of experimental autoimmune encephalomyelitis, neuritis and uveitis. Acta Neuropathol (Berl) 97:119-26 (1999). PubMed: 9928822
- Schluesener HJ et al. Allograft-inflammatory factor-1 in rat experimental autoimmune encephalomyelitis, neuritis, and uveitis: expression by activated macrophages and microglial cells. Glia 24:244-51 (1998). PubMed: 9728770