FITC Anti-IgE antibody [BE5] (ab49434)

Submit a review Q&A (1)References (1)

Key features and details

  • FITC Mouse monoclonal [BE5] to IgE
  • Reacts with: Human
  • Conjugation: FITC. Ex: 493nm, Em: 528nm
  • Isotype: IgG1

Overview

  • Product name

    FITC Anti-IgE antibody [BE5]
    See all IgE primary antibodies

  • Description

    FITC Mouse monoclonal [BE5] to IgE

  • Host species

    Mouse

  • Conjugation

    FITC. Ex: 493nm, Em: 528nm

  • Specificity

    This antibody reacts with IgE

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Full length native protein (purified) corresponding to Human IgE.

  • General notes

    The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    pH: 7.4
    Preservative: 0.097% Sodium azide
    Constituents: PBS, 0.2% BSA
  • Concentration information loading...

  • Purity

    Size exclusion

  • Clonality

    Monoclonal

  • Clone number

    BE5

  • Isotype

    IgG1

  • Research areas

Target

  • Relevance

    IgE is the class of antibodies produced in the lungs, skin, and mucous membranes. It may protect against parasite invasion, but it is a major factor in allergic reactions. The antigen-specific IgE interacts with mast cells and eosinophils, triggers the release of histamine, leukotrienes and other substances that lead to the itching, sneezing and congestion of allergies - and the life threatening respiratory distress of asthma and anaphylactic shock.

  • Cellular localization

    Cell Membrane

  • Database links

    • Alternative names

      • Igh2 antibody
      • IGHE antibody
      • IGHEP1 antibody
      • Immunoglobulin heavy constant epsilon antibody

    References (1)

    Publishing research using ab49434? Please let us know so that we can cite the reference in this datasheet.

    ab49434 has been referenced in 1 publication.

    • Gervais FG  et al. Pharmacological characterization of MK-7246, a potent and selective CRTH2 (chemoattractant receptor-homologous molecule expressed on T-helper type 2 cells) antagonist. Mol Pharmacol 79:69-76 (2011). Flow Cyt ; Human . PubMed: 20943773

    Customer reviews and Q&As

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    Question

    Hi I was wondering whether you could tell me which epitope this antibody binds to? We are conducting some experiments whereby we are crosslinking the IgE-receptor and then looking at expression but need to know that the epitope recognised by your antibody is not the same one as the antibody that we are using t stimulate our cells. Many thanks in advance for your assistance

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    Answer

    Unfortunately, having consulted with the source of this antibody, the epitope of the antibody is not known. However, it has been established that the antibody detects a different epitope to that of clones 4G7 and 4H10 (4G7: ab774 and 4H10: ab774 and ab77121 PE-dynamics conjugated). If you find there is an overlap with ab49434 and your other antibody you could change to using one of these alternatives. Otherwise we have a few anti-human IgE of which the epitope is know. ab7294 and ab7295 are specific for the Ce3 and Ce2 region respectively. ab7378 is specific for the Fc region and ab7382 is specific for the epsilon chain. I hope this information has been of help to you. If you have any further questions please do not hesitate to ask.

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