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FITC Anti-Integrin alpha 6 antibody [450-30A] (ab30496)

Submit a review Q&A (1)References (4)
Flow Cytometry - FITC Anti-Integrin alpha 6 antibody [450-30A] (ab30496)

    Key features and details

    • FITC Mouse monoclonal [450-30A] to Integrin alpha 6
    • Suitable for: Flow Cyt
    • Reacts with: Human
    • Conjugation: FITC. Ex: 493nm, Em: 528nm
    • Isotype: IgG1

    Overview

    • Product name

      FITC Anti-Integrin alpha 6 antibody [450-30A]
      See all Integrin alpha 6 primary antibodies

    • Description

      FITC Mouse monoclonal [450-30A] to Integrin alpha 6

    • Host species

      Mouse

    • Conjugation

      FITC. Ex: 493nm, Em: 528nm

    • Tested applications

      Suitable for: Flow Cytmore details

    • Species reactivity

      Reacts with: Human

    • Immunogen

      Full length native protein (Human): alpha 6 beta 4 integrin purified from A431 cells

    • Positive control

      • human peripheral blood lymphocytes

    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab30496 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt
    Use 10µl for 106 cells.

    ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

     
    Notes
    Flow Cyt
    Use 10µl for 106 cells.

    ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

     

    Target

    • Function

      Integrin alpha-6/beta-1 is a receptor for laminin on platelets. Integrin alpha-6/beta-4 is a receptor for laminin in epithelial cells and it plays a critical structural role in the hemidesmosome (By similarity). ITGA6:ITGB4 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling (PubMed:20682778). ITGA6:ITGB4 binds to IGF1 and this binding is essential for IGF1 signaling (PubMed:22351760).

    • Tissue specificity

      Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoforms containing segment X1 are ubiquitously expressed. Isoforms containing segment X1X2 are expressed in heart, kidney, placenta, colon, duodenum, myoblasts and myotubes, and in a limited number of cell lines; they are always coexpressed with the ubiquitous isoform containing segment X1. In some tissues (e.g. Salivary gland), isoforms containing cytoplasmic segment A and isoforms containing segment B are detected while in others, only isoforms containing one cytoplasmic segment are found (segment A in epidermis and segment B in kidney).

    • Involvement in disease

      Epidermolysis bullosa letalis, with pyloric atresia

    • Sequence similarities

      Belongs to the integrin alpha chain family.
      Contains 7 FG-GAP repeats.

    • Post-translational
      modifications

      Isoforms containing segment A, but not segment B, are the major targets for PMA-induced phosphorylation. Phosphorylation occurs on 'Ser-1103' of isoform alpha-6X1X2A. Phosphorylation is not required for the induction of integrin alpha-6A/beta-1 high affinity but may reduce the affinity for ligand.
      In invasive prostate cancer ITGA6 undergoes PLAU-mediated cleavage at residues Arg-634-635-Arg in a time-dependent manner enhancing cell invasion and migration in vitro.
      Palmitoylation by DHHC3 enhances stability and cell surface expression.

    • Cellular localization

      Cell membrane. Cell membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • Alpha6p antibody
        • CD49 antigen-like family member F antibody
        • CD49f antibody
        • Integrin alpha6B antibody
        • Integrin, alpha 6 antibody
        • ITA6_HUMAN antibody
        • ITGA6 antibody
        • ITGA6B antibody
        • Processed integrin alpha-6 antibody
        • VLA 6 antibody
        • VLA-6 antibody
        see all

      Images

      • Flow Cytometry - FITC Anti-Integrin alpha 6 antibody [450-30A] (ab30496)
        Flow Cytometry - FITC Anti-Integrin alpha 6 antibody [450-30A] (ab30496)
        Flow cytometric analysis of peripheral blood lymphocytes with 10µl for 106 cells of anti human Integrin alpha 6 FITC conjugated (ab30496).

      References (4)

      Publishing research using ab30496? Please let us know so that we can cite the reference in this datasheet.

      ab30496 has been referenced in 4 publications.

      • Woappi Y  et al. Establishing a High Throughput Epidermal Spheroid Culture System to Model Keratinocyte Stem Cell Plasticity. J Vis Exp N/A:N/A (2021). PubMed: 33586700
      • Woappi Y  et al. Self-assembling 3D spheroid cultures of human neonatal keratinocytes have enhanced regenerative properties. Stem Cell Res 49:102048 (2020). PubMed: 33128954
      • Sobral LM  et al. SET/I2PP2A overexpression induces phenotypic, molecular, and metabolic alterations in an oral keratinocyte cell line. FEBS J 284:2774-2785 (2017). PubMed: 28636114
      • Calenic B  et al. Magnetic separation and characterization of keratinocyte stem cells from human gingiva. J Periodontal Res 45:703-8 (2010). ICC/IF, Flow Cyt ; Human . PubMed: 20546107

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      Question

      I would like to test ab30496 for use on mouse samples.

      Read More
      Answer

      DISCOUNT CODE: ***
      Expiration date: June 15th, 2012

      I am very pleased to hear you would like to accept our offer and test ab30496 in mouse. This code will give you 1 freeprimary antibodybefore the expiration date. To redeem this offer, please submit an Abreview for mouse and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

      Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

      Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

      The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

      Read More

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com