FITC Anti-Integrin beta 7 antibody [FIB504] (ab95818)
![Flow Cytometry - FITC Anti-Integrin beta 7 antibody [FIB504] (ab95818)](https://www.abcam.com/ps/products/95/ab95818/Images/Integrin-beta-7-Primary-antibodies-ab95818-1.jpg "Flow Cytometry - FITC Anti-Integrin beta 7 antibody [FIB504] (ab95818)")
Key features and details
- FITC Rat monoclonal [FIB504] to Integrin beta 7
- Suitable for: Flow Cyt
- Reacts with: Mouse, Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG2a
Overview
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Product name
FITC Anti-Integrin beta 7 antibody [FIB504]
See all Integrin beta 7 primary antibodies -
Description
FITC Rat monoclonal [FIB504] to Integrin beta 7 -
Host species
Rat -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
TK1 murine t-cell lymphoma
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Positive control
- Normal Human peripheral blood cells
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
FIB504 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab95818 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use 5µl for 106 cells. ab18446 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|
Target
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Function
Integrin alpha-4/beta-7 (Peyer patches-specific homing receptor LPAM-1) is an adhesion molecule that mediates lymphocyte migration and homing to gut-associated lymphoid tissue (GALT). Integrin alpha-4/beta-7 interacts with the cell surface adhesion molecules MADCAM1 which is normally expressed by the vascular endothelium of the gastrointestinal tract. Interacts also with VCAM1 and fibronectin, an extracellular matrix component. It recognizes one or more domains within the alternatively spliced CS-1 region of fibronectin. Interactions involves the tripeptide L-D-T in MADCAM1, and L-D-V in fibronectin. Binds to HIV-1 gp120, thereby allowing the virus to enter GALT, which is thought to be the major trigger of AIDS disease. Interaction would involve a tripeptide L-D-I in HIV-1 gp120. Integrin alpha-E/beta-7 (HML-1) is a receptor for E-cadherin. -
Tissue specificity
Expressed in a variety of leukocyte lines. -
Sequence similarities
Belongs to the integrin beta chain family.
Contains 1 VWFA domain. -
Domain
Domain I contains three cation-binding sites: the ligand-integrin-binding site (LIMBS), the metal ion-dependent adhesion site (MIDAS), and the adjacent to MIDAS site (ADMIDAS). In the absence of a ligand or in calcium-dependent binding, only ADMIDAS is occupied. In magnesium-dependent binding all three sites bind metal ions. LIMBS positively modify ligand binding whereas ADMIDAS negatively modify ligand binding. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 3695 Human
- Entrez Gene: 16421 Mouse
- Omim: 147559 Human
- SwissProt: P26010 Human
- SwissProt: P26011 Mouse
- Unigene: 654470 Human
- Unigene: 58 Mouse
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Alternative names
- Gut homing receptor beta subunit antibody
- Integrin beta 7 subunit antibody
- Integrin beta-7 antibody
see all
Images
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Flow Cytometry - FITC Anti-Integrin beta 7 antibody [FIB504] (ab95818)Staining of normal Human peripheral blood cells with Rat IgG2a ? Isotype Control FITC (open histogram) or ab95818 (filled histogram). Cells in the lymphocyte gate were used for analysis.
Datasheets and documents
References (1)
ab95818 has been referenced in 1 publication.
- Meerschaert J et al. Engagement of alpha4beta7 integrins by monoclonal antibodies or ligands enhances survival of human eosinophils in vitro. J Immunol 163:6217-27 (1999). PubMed: 10570314
