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    fitc-interferon-gamma-antibody-xmg12-ab210392.pdf

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Immunology Innate Immunity Cytokines Interferons
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FITC Anti-Interferon gamma antibody [XMG1.2] (ab210392)

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Flow Cytometry - FITC Anti-Interferon gamma antibody [XMG1.2] (ab210392)

    Key features and details

    • FITC Rat monoclonal [XMG1.2] to Interferon gamma
    • Suitable for: Flow Cyt
    • Reacts with: Mouse
    • Conjugation: FITC. Ex: 493nm, Em: 528nm
    • Isotype: IgG1

    You may also be interested in

    Protein
    Product image
    Recombinant Human Interferon gamma protein (Active) (ab259377)

    View more associated products

    Overview

    • Product name

      FITC Anti-Interferon gamma antibody [XMG1.2]
      See all Interferon gamma primary antibodies
    • Description

      FITC Rat monoclonal [XMG1.2] to Interferon gamma
    • Host species

      Rat
    • Conjugation

      FITC. Ex: 493nm, Em: 528nm
    • Tested applications

      Suitable for: Flow Cytmore details
    • Species reactivity

      Reacts with: Mouse
    • Immunogen

      The details of the immunogen for this antibody are not available.

    • Positive control

      • C57Bl/6 splenocytes stimulated with PMA and Ionomycin.
    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C. Store In the Dark.
    • Storage buffer

      pH: 7.20
      Preservative: 0.09% Sodium azide
      Constituents: 0.12% Monobasic dihydrogen sodium phosphate, 0.87% Sodium chloride, 0.1% Gelatin
    • Concentration information loading...
    • Purity

      Protein A purified
    • Purification notes

      ab210392 was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.
    • Clonality

      Monoclonal
    • Clone number

      XMG1.2
    • Isotype

      IgG1
    • Light chain type

      kappa
    • Research areas

      • Immunology
      • Innate Immunity
      • Cytokines
      • Interferons
      • Stem Cells
      • Signaling Pathways
      • TGF beta
      • Interferon gamma
      • Cancer
      • Tumor immunology
      • Cytokines
      • Interferons
      • Immunology
      • Adaptive Immunity
      • Regulatory T Cells

    Associated products

    • Alternative Versions

      • APC Anti-Interferon gamma antibody [XMG1.2] (ab210390)
      • Anti-Interferon gamma antibody [XMG1.2] (ab23637)
      • violetFluor™ 450 Anti-Interferon gamma antibody [XMG1.2] (ab253083)
    • Isotype control

      • FITC Rat IgG1, kappa [RTK2071] - isotype control (ab18404)
    • Recombinant Protein

      • Recombinant Human Interferon gamma protein (Active) (ab259377)

    Applications

    Our Abpromise guarantee covers the use of ab210392 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt Use at an assay dependent concentration.

    Use 0.125μg.

    Target

    • Function

      Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
    • Tissue specificity

      Released primarily from activated T lymphocytes.
    • Involvement in disease

      In Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis.
    • Sequence similarities

      Belongs to the type II (or gamma) interferon family.
    • Post-translational
      modifications

      Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161.
    • Cellular localization

      Secreted.
    • Target information above from: UniProt accession P01579 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 15978 Mouse
      • SwissProt: P01580 Mouse
      • Unigene: 240327 Mouse
      • Alternative names

        • IF 1 antibody
        • IFG antibody
        • IFI antibody
        • IFN gamma antibody
        • IFN immune antibody
        • IFN, immune antibody
        • IFN-gamma antibody
        • IFNG antibody
        • IFNG_HUMAN antibody
        • Immune interferon antibody
        • Interferon gamma antibody
        • Type II Interferon antibody
        see all

      Images

      • Flow Cytometry - FITC Anti-Interferon gamma antibody [XMG1.2] (ab210392)
        Flow Cytometry - FITC Anti-Interferon gamma antibody [XMG1.2] (ab210392)

        Flow cytometric analysis of C57Bl/6 splenocytes stimulated with PMA and Ionomycin (right panel) or unstimulated (left panel), stained with APC Anti-Mouse CD3e, followed by intracellular labeling of IFN gamma with 0.125 µg ab210392.

      Protocols

      • Flow cytometry protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (0)

      Publishing research using ab210392? Please let us know so that we can cite the reference in this datasheet.

      ab210392 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

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