FITC Anti-Interferon gamma antibody [XMG1.2] (ab210392)

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Flow Cytometry - FITC Anti-Interferon gamma antibody [XMG1.2] (ab210392)

    Key features and details

    • FITC Rat monoclonal [XMG1.2] to Interferon gamma
    • Suitable for: Flow Cyt
    • Reacts with: Mouse
    • Conjugation: FITC. Ex: 493nm, Em: 528nm
    • Isotype: IgG1

    Overview

    • Product name

      FITC Anti-Interferon gamma antibody [XMG1.2]
      See all Interferon gamma primary antibodies

    • Description

      FITC Rat monoclonal [XMG1.2] to Interferon gamma

    • Host species

      Rat

    • Conjugation

      FITC. Ex: 493nm, Em: 528nm

    • Tested Applications & Species

      Application Species
      Flow Cyt
      Mouse
      See all applications and species data

    • Immunogen

      The details of the immunogen for this antibody are not available.

    • Positive control

      • C57Bl/6 splenocytes stimulated with PMA and Ionomycin.

    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

      One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

      Learn more here.

    Properties

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab210392 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    Flow Cyt
    Mouse
    Application Abreviews Notes
    Flow Cyt
    Use at an assay dependent concentration.

    Use 0.125μg.
    Notes
    Flow Cyt
    Use at an assay dependent concentration.

    Use 0.125μg.

    Target

    • Function

      Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.

    • Tissue specificity

      Released primarily from activated T lymphocytes.

    • Involvement in disease

      In Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis.

    • Sequence similarities

      Belongs to the type II (or gamma) interferon family.

    • Post-translational
      modifications

      Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161.

    • Cellular localization

      Secreted.
    • Information by UniProt

    • Database links

      • Alternative names

        • IF 1 antibody
        • IFG antibody
        • IFI antibody
        • IFN gamma antibody
        • IFN immune antibody
        • IFN, immune antibody
        • IFN-gamma antibody
        • IFNG antibody
        • IFNG_HUMAN antibody
        • Immune interferon antibody
        • Interferon gamma antibody
        • Type II Interferon antibody
        see all

      Images

      • Flow Cytometry - FITC Anti-Interferon gamma antibody [XMG1.2] (ab210392)
        Flow Cytometry - FITC Anti-Interferon gamma antibody [XMG1.2] (ab210392)

        Flow cytometric analysis of C57Bl/6 splenocytes stimulated with PMA and Ionomycin (right panel) or unstimulated (left panel), stained with APC Anti-Mouse CD3e, followed by intracellular labeling of IFN gamma with 0.125 µg ab210392.

      References (0)

      Publishing research using ab210392? Please let us know so that we can cite the reference in this datasheet.

      ab210392 has not yet been referenced specifically in any publications.

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