Key features and details
- FITC Mouse monoclonal [17B4] to LAG-3
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG1
Product nameFITC Anti-LAG-3 antibody [17B4]
See all LAG-3 primary antibodies
DescriptionFITC Mouse monoclonal [17B4] to LAG-3
ConjugationFITC. Ex: 493nm, Em: 528nm
Tested applicationsSuitable for: Flow Cytmore details
Species reactivityReacts with: Human
EpitopeRecognizes the 30 aa extra-loop of the first N-terminal D1 domain.
- Flow cyt: Tumor infiltrating lymphocytes; Activated human peripheral blood mononuclear cells.
This product was previously labelled as Lymphocyte Activation Gene 3.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C. Store In the Dark.
Storage bufferPreservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 0.79% Tris HCl
Concentration information loading...
PurityProtein A purified
Purification notesPurified from concentrated hybridoma tissue culture supernatant.
Our Abpromise guarantee covers the use of ab252271 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
FunctionInvolved in lymphocyte activation. Binds to HLA class-II antigens.
Tissue specificityOn cell surface of activated NK and T-lymphocytes.
Sequence similaritiesContains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
- Information by UniProt
- CD223 antibody
- CD223 antigen antibody
- FDC protein antibody
Tumor infiltrating lymphocytes (TILs) express LAG-3 detected using ab252271.
Method: Freshly dissociated single cell suspensions of renal cell carcinoma TILs are incubated with ab252271 at 5 µg/ml and anti-MHC Class II molecules (PE) for 30 mins and washed twice in saline buffer.
Additional staining with anti-CD3 allowed a gate analysis of total T cells.
The LAG-3 and MHC II profiles of CD3+-gated cells for 3 patients are shown.
Specific inhibition of 17B4 staining.
Method: ab252271 at 10 µg/ml was preincubated with a specific peptide epitope (208b) or a control tetanus toxoid (TT) peptide at different molarities prior to staining of TILs. Stained cells are then analyzed by FACS.
LAG-3 expression on activated human peripheral blood mononuclear cells (PBMC) detected with ab252271.
Method: T lymphocytes from human PBMC are stimulated with 1 µg/ml of PHA for three days. Then, after seven days of culture, 3x106 three-days PHA-activated human PBMC are treated with ab252271 or FITC coupled isotype-matched (IgG1) control MAb (used at a saturating dilution of 1/800 and 1/150 respectively) for 30 mins at 4°C in RPMI 1640 and washed twice with 1x PBS. Stained cells are then analysed by FACS.
Expression of LAG-3 on CD4+ and CD8+ subpopulations of tumour infiltrating lymphocytes (TILs) detected with ab252271.
Method: TILs from a dissociated renal cell carcinoma sample, stained with 5 µg/ml ab252271 and FITC-coupled anti-CD4 or -CD8, are analyzed by a two-colour FACS analysis. Additional staining with anti-CD3 allowed a gate analysis of total T cells. Values indicate percentages in each quadrant.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab252271 has not yet been referenced specifically in any publications.