FITC Anti-LAIR1 antibody [NKTA255] (ab27744)

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Flow Cytometry - FITC Anti-LAIR1 antibody [NKTA255] (ab27744)

    Key features and details

    • FITC Mouse monoclonal [NKTA255] to LAIR1
    • Suitable for: Flow Cyt
    • Reacts with: Human
    • Conjugation: FITC. Ex: 493nm, Em: 528nm
    • Isotype: IgG1

    Overview

    • Product name

      FITC Anti-LAIR1 antibody [NKTA255]
      See all LAIR1 primary antibodies

    • Description

      FITC Mouse monoclonal [NKTA255] to LAIR1

    • Host species

      Mouse

    • Conjugation

      FITC. Ex: 493nm, Em: 528nm

    • Tested applications

      Suitable for: Flow Cytmore details

    • Species reactivity

      Reacts with: Human

    • Immunogen

      NK cells lines B12.100 and AM.25 (Human)

    • General notes


      Monoclonal antibodies of clone NKTA255, such as ab27744, are reported to inhibit NK cell triggering via CD16 molecules.

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Store at +4°C.

    • Storage buffer

      pH: 7.40
      Preservative: 0.09% Sodium azide
      Constituents: PBS, 1% BSA
    • Concentration information loading...

    • Purity

      Protein G purified

    • Primary antibody notes

      Monoclonal antibodies of clone NKTA255, such as ab27744, are reported to inhibit NK cell triggering via CD16 molecules.

    • Clonality

      Monoclonal

    • Clone number

      NKTA255

    • Myeloma

      P3U1

    • Isotype

      IgG1

    • Research areas

    Applications

    Our Abpromise guarantee covers the use of ab27744 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt Use 10µl for 106 cells.

    10µl of neat antibody for 106 cells in 100µl.

     

     

     

     

    ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

     

    Target

    • Function

      Functions as an inhibitory receptor that plays a constitutive negative regulatory role on cytolytic function of natural killer (NK) cells, B-cells and T-cells. Activation by Tyr phosphorylation results in recruitment and activation of the phosphatases PTPN6 and PTPN11. It also reduces the increase of intracellular calcium evoked by B-cell receptor ligation. May also play its inhibitory role independently of SH2-containing phosphatases. Modulates cytokine production in CD4+ T-cells, downregulating IL2 and IFNG production while inducing secretion of transforming growth factor beta. Down-regulates also IgG and IgE production in B-cells as well as IL8, IL10 and TNF secretion. Inhibits proliferation and induces apoptosis in myeloid leukemia cell lines as well as prevents nuclear translocation of NF-kappa-B p65 subunit/RELA and phosphorylation of I-kappa-B alpha/CHUK in these cells. Inhibits the differentiation of peripheral blood precursors towards dendritic cells.

    • Tissue specificity

      Expressed on the majority of peripheral mononuclear cells, including natural killer (NK) cells, T-cells, B-cells, monocytes, and dendritic cells. Highly expressed in naive T-cells and B-cells but no expression on germinal center B-cells. Abnormally low expression in naive B-cells from HIV-1 infected patients. Very low expression in NK cells from a patient with chronic active Epstein-Barr virus infection.

    • Sequence similarities

      Contains 1 Ig-like C2-type (immunoglobulin-like) domain.

    • Developmental stage

      Complete loss of expression when naive B-cells proliferates and differentiates into Ig-producing plasma cells under in vitro stimulation.

    • Domain

      ITIM (immunoreceptor tyrosine-based inhibitor motif) motif is a cytoplasmic motif present in 2 copies in the intracellular part of LAIR1. When phosphorylated, ITIM motif can bind the SH2 domain of several SH2-containing phosphatases, leading to down-regulation of cell activation.

    • Post-translational
      modifications

      Phosphorylation at Tyr-251 and Tyr-281 activates it. May be phosphorylated by LCK.
      N-glycosylated.

    • Cellular localization

      Cell membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • 5133400O11Rik antibody
        • CD305 antibody
        • D7Bwg0421e antibody
        • hLAIR1 antibody
        • LAIR 1 antibody
        • LAIR-1 antibody
        • LAIR1 antibody
        • LAIR1 protein antibody
        • LAIR1_HUMAN antibody
        • Leukocyte associated Ig like receptor 1 antibody
        • Leukocyte associated immunoglobulin like receptor 1 antibody
        • Leukocyte-associated immunoglobulin-like receptor 1 antibody
        see all

      Images

      • Flow Cytometry - FITC Anti-LAIR1 antibody [NKTA255] (ab27744)
        Flow Cytometry - FITC Anti-LAIR1 antibody [NKTA255] (ab27744)

        Flow Cytometry analysis of human peripheral blood lymphocytes labeling LAIR1 with FITC-conjugated ab27744.

      References (2)

      Publishing research using ab27744? Please let us know so that we can cite the reference in this datasheet.

      ab27744 has been referenced in 2 publications.

      • Exley M  et al. CD161 (NKR-P1A) costimulation of CD1d-dependent activation of human T cells expressing invariant V alpha 24 J alpha Q T cell receptor alpha chains. J Exp Med 188:867-76 (1998). PubMed: 9730888
      • Poggi A  et al. p40, a novel surface molecule involved in the regulation of the non-major histocompatibility complex-restricted cytolytic activity in humans. Eur J Immunol 25:369-76 (1995). PubMed: 7875198

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