FITC Anti-Lambda Light chain antibody (ab4211)
Key features and details
- FITC Rabbit polyclonal to Lambda Light chain
- Suitable for: IHC-P
- Reacts with: Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG
Overview
-
Product name
FITC Anti-Lambda Light chain antibody
See all Lambda Light chain primary antibodies -
Description
FITC Rabbit polyclonal to Lambda Light chain -
Host species
Rabbit -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length native protein (purified) corresponding to Human Lambda Light chain. Pool of human Lambda light chain isolated from the urine of patients with Bence Jones Proteinuria.
-
Positive control
- IHC-P: Human tonsil tissue.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.3
Preservative: 0.05% Sodium azide
Constituent: 1% BSA -
Concentration information loading... -
Purity
IgG fraction -
Purification notes
Traces of contaminating antibodies have been removed by solid phase absorption. Only the lambda precipitation line appeared in cross immunoelectrophoresis. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab4211 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/20 - 1/40.
Pretreat tissue with Proteinase K for 30 minutes at 37°C. Incubate with primary antibody for 30-60 minutes at room temperature. |
| Notes |
|---|
|
IHC-P
1/20 - 1/40. Pretreat tissue with Proteinase K for 30 minutes at 37°C. Incubate with primary antibody for 30-60 minutes at room temperature. |
Target
-
Relevance
All five immunoglobulin classes share the same basic four polypeptide chain structure of two heavy-chains and two light chains. There are five heavy chain types, and two light-chain types (Kappa and Lambda) both having a molecular weight of 22.5kDa. Any heavy-chain type can associate with either light-chain type, but on any immunoglobulin molecule both light-chains are of the same type. Kappa and Lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of Kappa to Lambda is 70:30 , the vast majority of which is bound to heavy-chain in immunoglobulin. In normal individuals low levels of free light-chain arepresent in serum (kappa, 1.6-15.2 mg/L; Lambda, 0.4-4.2mg/L), with the occurrence of multiple myeloma or other B-cell malignancies these levels can be greatly elevated and can be found at high levels in the urine (Bence-Jones proteins). -
Cellular localization
Cytoplasmic -
Database links
- Entrez Gene: 3535 Human
- Entrez Gene: 3546 Human
- Entrez Gene: 8217 Human
- Omim: 147220 Human
- SwissProt: P01701 Human
- SwissProt: P01842 Human
-
Alternative names
- Bence Jones protein antibody
- BJP antibody
- Constant region of lambda light chains antibody
see all
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FITC Anti-Lambda Light chain antibody (ab4211)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue labeling Lambda Light chain with ab4211.
Datasheets and documents
-
SDS download
-
Datasheet download
References (1)
ab4211 has been referenced in 1 publication.
- Kang SU et al. DIM-C-pPhtBu induces lysosomal dysfunction and unfolded protein response - mediated cell death via excessive mitophagy. Cancer Lett 504:23-36 (2021). PubMed: 33556544