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  1. Link

    fitc-legionella-pneumophila-antibody-ab20818.pdf

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Microbiology Organism Bacteria Gram negative Bacteria Legionella
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FITC Anti-Legionella pneumophila antibody (ab20818)

  • Datasheet
  • SDS
Submit a review Q&A (1)References (2)

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Key features and details

  • FITC Rabbit polyclonal to Legionella pneumophila
  • Suitable for: ICC/IF
  • Conjugation: FITC. Ex: 493nm, Em: 528nm
  • Isotype: IgG

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Conjugation
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HRP Anti-Respiratory Syncytial Virus antibody (ab20686)
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Anti-Legionella pneumophila antibody (ab20943)

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Overview

  • Product name

    FITC Anti-Legionella pneumophila antibody
    See all Legionella pneumophila primary antibodies
  • Description

    FITC Rabbit polyclonal to Legionella pneumophila
  • Host species

    Rabbit
  • Conjugation

    FITC. Ex: 493nm, Em: 528nm
  • Tested applications

    Suitable for: ICC/IFmore details
  • Species reactivity

    Reacts with: Other species
  • Immunogen

    Tissue, cells or virus corresponding to Legionella pneumophila. A whole cell preparation of Legionella pneumophila (ATCC 33152)

  • General notes

    Purified IgG fraction is covalently coupled with high purity Isomer I of fluorescein isothiocyanate. Care is taken to ensure complete removal of any free fluorescein from the final product.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.1% Sodium azide
    Constituents: 0.0268% PBS, 1% BSA
  • Concentration information loading...
  • Purity

    IgG fraction
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Microbiology
    • Organism
    • Bacteria
    • Gram negative Bacteria
    • Legionella

Associated products

  • Isotype control

    • FITC-Rabbit IgG - Isotype Control (ab37406)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab20818 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
Use at an assay dependent dilution.
Notes
ICC/IF
Use at an assay dependent dilution.

Target

  • Relevance

    Legionella pneumophila is a flagellated gram negative bacterium found primarily in warm water environments. Legionella pneumophila causes a type of pneumonia called Legionnaire disease and a milder condition called Pontiac fever. Infection is acquired through inhalation of the microorganism in water mists (e.g. from air conditioning, cooling towers, whirlpool spas and showers mists). A number of risk factors for acquiring Legionnaire disease have been identified, including age, smoking, chronic lung disease, cancer, and immunosuppression.

Protocols

  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (2)

Publishing research using ab20818? Please let us know so that we can cite the reference in this datasheet.

ab20818 has been referenced in 2 publications.

  • Keserue HA  et al. Rapid detection of total and viable Legionella pneumophila in tap water by immunomagnetic separation, double fluorescent staining and flow cytometry. Microb Biotechnol 5:753-63 (2012). PubMed: 23062200
  • Füchslin HP  et al. Rapid and quantitative detection of Legionella pneumophila applying immunomagnetic separation and flow cytometry. Cytometry A 77:264-74 (2010). PubMed: 20099248

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

Question

We are developing a fast screening method for L. pneumophila based on immunostaining with FITC coupled surface antibody / immunomagnetic separation and flow cytometric detection. We got very good recovery rate but one problem is that background and positive signal are quite near together. Therefore we want to double stain or magnify the fluorescence of the FITC-labelled L. pneumophila cells. We label our cells with one of your FITC conjugated antibody for L. pneumophila ( Abcam ab20818). We are working with a Flow cytometer (Cyflow Partec) with three lasers ( UV(375): 25 mW, Blue laser ( 488 nm): 200 mW, Green Laser (525 nm): 25 mW). I worked out two possible solutions: 1. Signal Amplification (520 nm): Anti-FITC coupled with Alexa Fluor 488 conjugate Question: 1. Can you recommend an Anti-FITC antibody for amplifying the signal? 2. I would be also interested for Anti-FITC antibody conjugated with other stain wich can ecited by UV- / Blue- or Greenlaser and excite at 375 nm , 520 nm , 590 nm or 630 nm. 2. Doublestain a) Specific antibody for L. pneumophila with a conjugate which can be excited by UV- or Green laser. Question: 1. Can you recommend me an antibody for L. pneumophila which is conjugated with another stain than FITC? 2. With a Antibody labelling Kit from Invitrogen I can label unconjugated IgG antibody for L. pneumphila. I see that you have several unconjugated antibody for L. pneumophila. Which one do you recommend me. I suppose that if possible it would be better to use another antibody as used my first antibody (Abcam ab20818) in order to avoid/minimise a competition for free epitopes on the surface of L. pneumophila.

Read More

Abcam community

Verified customer

Asked on May 14 2007

Answer

Thank you for contacting us and for presenting your questions so clearly! To amplify the the signal from the FITC-conjugated anti-Legionella antibody, your idea of using an Alexa Fluor 488-conjugated anti-FITC is a good one. We unfortunately do not have Alexa Fluor conjugates but these dyes have a good reputation for strong, stable signal. Another option is to follow the primary antibody with a biotinylated anti-rabbit IgG such as ab6720, and then a streptavidin or avidin fluorochrome conjugate. Again, we do not have these streptavidin/avidin/fluorochrome conjugates but they are available from other companies. The anti-rabbit IgG secondary will bind ab20818 even though it has FITC conjugated to it: https://www.abcam.com/Goat-Rabbit-IgG-HL-Biotin-ab6720.html We do have a phycoerythrin-conjugated anti-FITC, ab25539: https://www.abcam.com/PE-FITC-antibody-ab25539.html We do not have another fluorochrome-conjugated anti-Legionella antibody but we do have a biotin conjugate, ab20527: https://www.abcam.com/Biotin-Legionella-pneumophila-antibody-ab20527.html This can be used with the streptavidin/avidin conjugates I mentioned above. An antibody appropriate for labelling should have no other proteins in the buffer it is delivered in, as these will compete with the antibody for the label. Another consideration is that, since you already have a rabbit polyclonal antibody that will recognize potentially several epitopes, and it is not clear what exactly on the bacterium it binds, another polyclonal antibody applied before this one may interfere with it, and vice versa. Your best strategy, aside from using two monoclonal antibodies, is to apply a monoclonal antibody first, such as ab20444: https://www.abcam.com/Legionella-pneumophila-antibody-266-ab20444.html This will bind a single epitope on a single protein. It may interfere somewhat with the rabbit antibody that follows it but chances are the rabbit antibody will recognize other proteins on the bacterium in addition to, or other than, what the monoclonal binds. Antibody ab20444 is also suitable for conjugation: it is purified and there are no other proteins in the solution. The order in which these antibodies are used is important. Do not apply the rabbit polyclonal ab20818 before the mouse monoclonal ab20444; the rabbit may very well hinder the mouse antibody. I hope this helps. Please let us know if you have any other questions or concerns.

Read More

Abcam Scientific Support

Answered on May 14 2007

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