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    fitc-listeria-monocytogenes-antibody-ab20861.pdf

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Microbiology Organism Bacteria Gram positive bacteria Listeria
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FITC Anti-Listeria monocytogenes antibody (ab20861)

  • Datasheet
  • SDS
Submit a review Q&A (3)

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Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Key features and details

  • FITC Rabbit polyclonal to Listeria monocytogenes
  • Suitable for: ELISA
  • Conjugation: FITC. Ex: 493nm, Em: 528nm
  • Isotype: IgG

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Conjugation
Product image
FITC Conjugation Kit - Lightning-Link® (ab102884)

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Overview

  • Product name

    FITC Anti-Listeria monocytogenes antibody
    See all Listeria monocytogenes primary antibodies
  • Description

    FITC Rabbit polyclonal to Listeria monocytogenes
  • Host species

    Rabbit
  • Conjugation

    FITC. Ex: 493nm, Em: 528nm
  • Specificity

    Recognizes whole cells. Antiserum is not absorbed and may react with other related microorganisms.
  • Tested applications

    Suitable for: ELISAmore details
  • Species reactivity

    Reacts with: Other species
  • Immunogen

    Tissue/ cell preparation. Listeria monocytogenes; ATCC #43251.

  • General notes

    Coupled with high purity Isomer I of fluorescein isothiocyanate. Care is taken to ensure complete removal of any free fluorescein from the final product.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.1% Sodium azide
    Constituents: 0.0268% PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Microbiology
    • Organism
    • Bacteria
    • Gram positive bacteria
    • Listeria

Associated products

  • Isotype control

    • FITC-Rabbit IgG - Isotype Control (ab37406)

Applications

Our Abpromise guarantee covers the use of ab20861 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
  • Application notes
    ELISA: Use at an assay dependent dilution.

    Enzyme amplification following reaction with FITC conjugate can also be accomplished utilizing enzyme-antibody conjugates specific to FITC.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance

      The genus Listeria comprises six species: L. monocytogenes, L. innocua, L. welshimeri, L. seeligeri, L. ivanovii and L. grayi. Listeria monocytogenes, the most commonly isolated pathogenic member, is associated with a wide spectrum of human and animal diseases. In the smear from the original tissue, L. monocytogenes may appear as gram-positive coccobacilli that may be confused with Streptococcus agalactiae (group B), enterococci, or Corynebacterium spp. Listeria is differentiated from streptococci by a positive catalase test. L. monocytogenes is the only species of the genus Listeria that has been clearly documented as a pathogen for humans. The forms of disease caused by this organism are myriad and age-related. The most common clinical manifestations are meningitis and septicemia. Listeria monocytogenes, a food-borne intracellular animal and human pathogen, interacts with infected host cells both prior to entry and during the intracellular phase of infection.
    • Alternative names

      • L. monocytogenes antibody

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (0)

    Publishing research using ab20861? Please let us know so that we can cite the reference in this datasheet.

    ab20861 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-3 of 3 Abreviews or Q&A

    Question

    Dear All, our customer is studying Listeria monocytogenes and she is interested in you antibodies but she would like to use them in live cells. Do your antibodies (here below) work in live cells?  

    Read More

    Abcam community

    Verified customer

    Asked on Oct 18 2011

    Answer

    Thank you for your enquiry. To help answer your customer's question, could you please let me know what specific application they will use the antibodies for (eg. western blotting, IHC, etc). Thanks!

    Read More

    Abcam Scientific Support

    Answered on Oct 18 2011

    Question

    How many FITC molecules are there per antibody?

    Read More

    Abcam community

    Verified customer

    Asked on Jun 02 2006

    Answer

    Thank you for your enquiry. This information has not been determined for this antibody. Please contact us again if you have any additional questions.

    Read More

    Abcam Scientific Support

    Answered on Jun 02 2006

    Question

    Is this antibody species specific? Or does it cross-react with other Listeria?

    Read More

    Abcam community

    Verified customer

    Asked on Mar 14 2006

    Answer

    Ab20861 recognizes whole cells. This antiserum is not absorbed and may react with other related microorganisms. The antibody cross-reacts with Group A Streptococcus, Group B Streptococcus, S. pneumoniae, Staph aureus, clostridium perfingens and Bacillus subtilis. Although ab20861 has not been tested to recognize other Lysteria species (except monocytogenes), the lab expects that it is likely to react with other species. Please contact us again if you have any additional questions.

    Read More

    Abcam Scientific Support

    Answered on Mar 14 2006

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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