FITC Anti-LLGL1 antibody [4D11] (ab95802)

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Flow Cytometry - FITC Anti-LLGL1 antibody [4D11] (ab95802)

    Key features and details

    • FITC Rat monoclonal [4D11] to LLGL1
    • Suitable for: Flow Cyt
    • Reacts with: Mouse
    • Conjugation: FITC. Ex: 493nm, Em: 528nm
    • Isotype: IgG2a

    Overview

    • Product name

      FITC Anti-LLGL1 antibody [4D11]
      See all LLGL1 primary antibodies

    • Description

      FITC Rat monoclonal [4D11] to LLGL1

    • Host species

      Rat

    • Conjugation

      FITC. Ex: 493nm, Em: 528nm

    • Tested applications

      Suitable for: Flow Cytmore details

    • Species reactivity

      Reacts with: Mouse

    • Immunogen

      Large granular lymphocytes (LGL) enriched from C57BL/6N mouse liver

    • Positive control

      • Mouse splenocytes.

    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    Applications

    Our Abpromise guarantee covers the use of ab95802 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt Use 0.125-0.25µg for 105-8 cells.

    Perform staining in a final volume of 100 µl.

     

     

     

    ab18446 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

     

    Target

    • Function

      Cortical cytoskeleton protein found in a complex involved in maintaining cell polarity and epithelial integrity. Involved in the regulation of mitotic spindle orientation, proliferation, differentiation and tissue organization of neuroepithelial cells.

    • Tissue specificity

      Expressed in brain, kidney, and muscle but is barely seen in heart and placenta. Down-regulated or lost in all cell lines and in most of the tumor samples analyzed. Loss was associated with advanced stage of the disease.

    • Sequence similarities

      Belongs to the WD repeat L(2)GL family.
      Contains 14 WD repeats.

    • Post-translational
      modifications

      Phosphorylated upon DNA damage, probably by ATM or ATR (By similarity). Phosphorylated at least at Ser-663 by PRKCI.

    • Cellular localization

      Cytoplasm > cytoskeleton. Localized to the lateral membrane during the polarization and formation cell-cell contacts.
    • Information by UniProt

    • Database links

      • Alternative names

        • DLG4 antibody
        • DLG4, formerly antibody
        • Hugl 1 antibody
        • HUGL antibody
        • Hugl-1 antibody
        • HUGL1 antibody
        • Human homolog to the D-lgl gene protein antibody
        • L2GL1_HUMAN antibody
        • Lethal giant larvae homolog 1 (Drosophila) antibody
        • Lethal giant larvae homolog 1 antibody
        • Lethal(2) giant larvae protein homolog 1 antibody
        • LLGL antibody
        • Llgl1 antibody
        • Ly 49G2 antibody
        • Ly49G2 antibody
        • Mgl1 antibody
        • Mlgl antibody
        see all

      Images

      • Flow Cytometry - FITC Anti-LLGL1 antibody [4D11] (ab95802)
        Flow Cytometry - FITC Anti-LLGL1 antibody [4D11] (ab95802)
        Staining of C57BL/6 splenocytes with Anti-Mouse CD49b (Integrin alpha2) PE and 0.125 µg of Rat IgG2a k Isotype Control FITC (left) or 0.125 µg of ab95802 (right). Total viable cells were used for analysis.

      References (3)

      Publishing research using ab95802? Please let us know so that we can cite the reference in this datasheet.

      ab95802 has been referenced in 3 publications.

      • Ortaldo JR  et al. The Ly-49 family: regulation of cytotoxicity and cytokine production in murine CD3+ cells. J Immunol 160:1158-65 (1998). PubMed: 9570529
      • Mason LH  et al. Cloning and functional characteristics of murine large granular lymphocyte-1: a member of the Ly-49 gene family (Ly-49G2) J Exp Med 182:293-303 (1995). PubMed: 7629495
      • Mason L  et al. LGL-1: a non-polymorphic antigen expressed on a major population of mouse natural killer cells. J Immunol 140:4403-12 (1988). PubMed: 2453582

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