FITC Anti-LOX 1 antibody [23C11] (ab81710)
![Flow Cytometry - FITC Anti-LOX 1 antibody [23C11] (ab81710)](https://www.abcam.com/ps/products/81/ab81710/Images/LOX-1-Primary-antibodies-ab81710-1.jpg "Flow Cytometry - FITC Anti-LOX 1 antibody [23C11] (ab81710)")
Key features and details
- FITC Mouse monoclonal [23C11] to LOX 1
- Suitable for: Flow Cyt
- Reacts with: Mouse, Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG1
Overview
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Product name
FITC Anti-LOX 1 antibody [23C11]
See all LOX 1 primary antibodies -
Description
FITC Mouse monoclonal [23C11] to LOX 1 -
Host species
Mouse -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Specificity
ab81710 reacts with LOX 1. -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Fusion protein of extracellular domain (aa 71-273) of human LOX-1 with murine Fcgamma1 (LOX-1-muFc) produced in PEAK cells
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Positive control
- Human APCs
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General notes
ab81710 neutralizes the biological activity of LOX 1. It also inhibits Hsp70 binding to dendritic cells and Hsp70 induced antigen cross representation. In vivo, targeting LOX1 with a tumour antigen using anti LOX 1 antibody ab81710 induces anti tumour immunity.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
Purification notes
Purified, 0.2 µm filtered solution. -
Primary antibody notes
ab81710 neutralizes the biological activity of LOX 1. It also inhibits Hsp70 binding to dendritic cells and Hsp70 induced antigen cross representation. In vivo, targeting LOX1 with a tumour antigen using anti LOX 1 antibody ab81710 induces anti tumour immunity. -
Clonality
Monoclonal -
Clone number
23C11 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab81710 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use 5µl for 106 cells. ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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Target
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Function
Receptor that mediates the recognition, internalization and degradation of oxidatively modified low density lipoprotein (oxLDL) by vascular endothelial cells. OxLDL is a marker of atherosclerosis that induces vascular endothelial cell activation and dysfunction, resulting in pro-inflammatory responses, pro-oxidative conditions and apoptosis. Its association with oxLDL induces the activation of NF-kappa-B through an increased production of intracellular reactive oxygen and a variety of pro-atherogenic cellular responses including a reduction of nitric oxide (NO) release, monocyte adhesion and apoptosis. In addition to binding oxLDL, it acts as a receptor for the HSP70 protein involved in antigen cross-presentation to naive T-cells in dendritic cells, thereby participating in cell-mediated antigen cross-presentation. Also involved in inflammatory process, by acting as a leukocyte-adhesion molecule at the vascular interface in endotoxin-induced inflammation. Also acts as a receptor for advanced glycation end (AGE) products, activated platelets, monocytes, apoptotic cells and both Gram-negative and Gram-positive bacteria. -
Tissue specificity
Expressed at high level in endothelial cells and vascular-rich organs such as placenta, lung, liver and brain, aortic intima, bone marrow, spinal cord and substantia nigra. Also expressed at the surface of dendritic cells. Widely expressed at intermediate and low level. -
Involvement in disease
Note=Independent association genetic studies have implicated OLR1 gene variants in myocardial infarction susceptibility.
Note=OLR1 may be involved in Alzheimer disease (AD). Involvement in AD is however unclear: according to some authors (PubMed:12354387, PubMed:12810610 and PubMed:15976314), variations in OLR1 modify the risk of AD, while according to other (PubMed:15000751 and PubMed:15060104) they do not. -
Sequence similarities
Contains 1 C-type lectin domain. -
Domain
The cytoplasmic region is required for subcellular sorting on the cell surface.
The C-type lectin domain mediates the recognition and binding of oxLDL. -
Post-translational
modificationsThe intrachain disulfide-bonds prevent N-glycosylation at some sites.
N-glycosylated. -
Cellular localization
Cell membrane. Secreted. A secreted form also exists. - Information by UniProt
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Database links
- Entrez Gene: 4973 Human
- Entrez Gene: 108078 Mouse
- Omim: 602601 Human
- SwissProt: P78380 Human
- SwissProt: Q9EQ09 Mouse
- Unigene: 412484 Human
- Unigene: 293626 Mouse
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Alternative names
- C-type lectin domain family 8 member A antibody
- CLEC8A antibody
- hLOX 1 antibody
see all
Images
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Flow Cytometry - FITC Anti-LOX 1 antibody [23C11] (ab81710)Overlay histogram showing A549 cells stained with ab81710 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab81710, 0.5µg/1x106 cells) for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 FITC (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
References (3)
ab81710 has been referenced in 3 publications.
- Barth H et al. Scavenger receptor class B is required for hepatitis C virus uptake and cross-presentation by human dendritic cells. J Virol 82:3466-79 (2008). PubMed: 18216094
- Jeannin P et al. Complexity and complementarity of outer membrane protein A recognition by cellular and humoral innate immunity receptors. Immunity 22:551-60 (2005). ICC/IF, Flow Cyt ; Human . PubMed: 15894273
- Delneste Y et al. Involvement of LOX-1 in dendritic cell-mediated antigen cross-presentation. Immunity 17:353-62 (2002). Human . PubMed: 12354387
