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Explore the power of knock-out cell lines for your research

  1. Link

    fitc-ly6c-antibody-hk14-ab25025.pdf

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Immunology Innate Immunity Macrophage / Inflamm.
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FITC Anti-Ly6c antibody [HK1.4] (ab25025)

  • Datasheet
  • SDS
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Key features and details

  • FITC Rat monoclonal [HK1.4] to Ly6c
  • Suitable for: IHC-Fr, Flow Cyt, Cellular Activation
  • Reacts with: Mouse
  • Conjugation: FITC. Ex: 493nm, Em: 528nm
  • Isotype: IgG2c

Conjugates logo Related conjugates and formulations

APC Biotin PE Unconjugated

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Recombinant Mouse Ly6c protein (Tagged) (ab240852)
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Anti-Ly6c antibody [HK1.4] (ab24973)

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Overview

  • Product name

    FITC Anti-Ly6c antibody [HK1.4]
    See all Ly6c primary antibodies
  • Description

    FITC Rat monoclonal [HK1.4] to Ly6c
  • Host species

    Rat
  • Conjugation

    FITC. Ex: 493nm, Em: 528nm
  • Specificity

    ab25025 recognises murine Ly-6C.
  • Tested applications

    Suitable for: IHC-Fr, Flow Cyt, Cellular Activationmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Tissue, cells or virus corresponding to Ly6c. L3 cloned CTL cells

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7.4
    Preservative: 0.1% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    HK1.4
  • Isotype

    IgG2c
  • Light chain type

    kappa
  • Research areas

    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.
    • Immunology
    • Adaptive Immunity
    • T Cells
    • Non-CD
    • Cell Biology
    • Cell Cycle
    • Cell differentiation
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • T Lymphocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Monocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Neutrophil Lineage

Associated products

  • Alternative Versions

    • Anti-Ly6c antibody [HK1.4] (ab24973)
    • Biotin Anti-Ly6c antibody [HK1.4] (ab24980)
    • PE Anti-Ly6c antibody [HK1.4] (ab25572)
    • APC Anti-Ly6c antibody [HK1.4] (ab93550)
  • Isotype control

    • FITC Rat IgG2c, kappa [RTK4174] - isotype control (ab114134)
  • Recombinant Protein

    • Recombinant Mouse Ly6c protein (Tagged) (ab240852)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab25025 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

ab114134 - Rat monoclonal IgG2c, is suitable for use as an isotype control with this antibody.

Cellular Activation
Use at an assay dependent concentration.

In vitro activation of T lymphocytes.

Notes
IHC-Fr
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

ab114134 - Rat monoclonal IgG2c, is suitable for use as an isotype control with this antibody.

Cellular Activation
Use at an assay dependent concentration.

In vitro activation of T lymphocytes.

Target

  • Relevance

    Ly6C is a monocyte/macrophage and endothelial cell differentiation antigen regulated by interferon gamma, and may play a role in the development and maturation of lymphocytes. It is a member of the Ly6 multigene family of type V glycophosphatidylinositol anchored cell surface proteins. It is expressed on bone marrow cells, monocytes/macrophages, neutrophils, endothelial cells, and T cell subsets. Mice with the Ly6.2 allotype (e.g., AKR, C57BL, C57BR, C57L, DBA/2, PL, SJL, SWR, 129) have subsets of CD4+Ly6C+ and CD8+Ly6C+ cells, while Ly6.1 strains (e.g., A, BALB/c, CBA, C3H/He, DBA/1, NZB) have only CD8+Ly6C+ lymphocytes.
  • Cellular localization

    Cell membrane; Lipid-anchor, GPI-anchor.
  • Database links

    • Entrez Gene: 17067 Mouse
    • SwissProt: P0CW02 Mouse
    • SwissProt: P0CW03 Mouse
    • Alternative names

      • Ly 6c antibody
      • Ly6c protein antibody
      • Ly6c1 antibody
      • Ly6c2 antibody
      • Lymphocyte antigen 6 complex antibody
      • Lymphocyte antigen 6 complex locus C antibody
      • Lymphocyte antigen 6C antibody
      • Lymphocyte antigen 6C1 antibody
      • Lymphocyte antigen 6C2 antibody
      • Lymphocyte antigen Ly 6C antibody
      see all

    Protocols

    • Flow cytometry protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (1)

    Publishing research using ab25025? Please let us know so that we can cite the reference in this datasheet.

    ab25025 has been referenced in 1 publication.

    • Deshmukh US  et al. Inflammatory stimuli accelerate Sjögren's syndrome-like disease in (NZB x NZW)F1 mice. Arthritis Rheum 58:1318-23 (2008). PubMed: 18438852

    Customer reviews and Q&As

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