FITC Anti-Ly6c antibody [HK1.4] (ab25025)
Key features and details
- FITC Rat monoclonal [HK1.4] to Ly6c
- Suitable for: IHC-Fr, Flow Cyt, Cellular Activation
- Reacts with: Mouse
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG2c
Related conjugates and formulations
Overview
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Product name
FITC Anti-Ly6c antibody [HK1.4]
See all Ly6c primary antibodies -
Description
FITC Rat monoclonal [HK1.4] to Ly6c -
Host species
Rat -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Specificity
ab25025 recognises murine Ly-6C. -
Tested applications
Suitable for: IHC-Fr, Flow Cyt, Cellular Activationmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Tissue, cells or virus corresponding to Ly6c. L3 cloned CTL cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.4
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
HK1.4 -
Isotype
IgG2c -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab25025 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr |
Use at an assay dependent concentration.
|
|
| Flow Cyt |
Use at an assay dependent concentration.
ab114134 - Rat monoclonal IgG2c, is suitable for use as an isotype control with this antibody. |
|
| Cellular Activation |
Use at an assay dependent concentration.
In vitro activation of T lymphocytes. |
| Notes |
|---|
|
IHC-Fr
Use at an assay dependent concentration. |
|
Flow Cyt
Use at an assay dependent concentration. ab114134 - Rat monoclonal IgG2c, is suitable for use as an isotype control with this antibody. |
|
Cellular Activation
Use at an assay dependent concentration. In vitro activation of T lymphocytes. |
Target
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Relevance
Ly6C is a monocyte/macrophage and endothelial cell differentiation antigen regulated by interferon gamma, and may play a role in the development and maturation of lymphocytes. It is a member of the Ly6 multigene family of type V glycophosphatidylinositol anchored cell surface proteins. It is expressed on bone marrow cells, monocytes/macrophages, neutrophils, endothelial cells, and T cell subsets. Mice with the Ly6.2 allotype (e.g., AKR, C57BL, C57BR, C57L, DBA/2, PL, SJL, SWR, 129) have subsets of CD4+Ly6C+ and CD8+Ly6C+ cells, while Ly6.1 strains (e.g., A, BALB/c, CBA, C3H/He, DBA/1, NZB) have only CD8+Ly6C+ lymphocytes. -
Cellular localization
Cell membrane; Lipid-anchor, GPI-anchor. -
Database links
- Entrez Gene: 17067 Mouse
- SwissProt: P0CW02 Mouse
- SwissProt: P0CW03 Mouse
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Alternative names
- Ly 6c antibody
- Ly6c protein antibody
- Ly6c1 antibody
see all
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab25025 has been referenced in 1 publication.
- Deshmukh US et al. Inflammatory stimuli accelerate Sjögren's syndrome-like disease in (NZB x NZW)F1 mice. Arthritis Rheum 58:1318-23 (2008). PubMed: 18438852