Key features and details
- FITC Mouse monoclonal [MEM-238] to M6PR (cation independent)
- Suitable for: WB, Flow Cyt
- Reacts with: Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG1
Product nameFITC Anti-M6PR (cation independent) antibody [MEM-238]
See all M6PR (cation independent) primary antibodies
DescriptionFITC Mouse monoclonal [MEM-238] to M6PR (cation independent)
ConjugationFITC. Ex: 493nm, Em: 528nm
Tested applicationsSuitable for: WB, Flow Cytmore details
Species reactivityReacts with: Human
Predicted to work with: Non human primates
Recombinant Vaccinia virus encoding Mannose 6 Phosphate Receptor (Cation independent) (CD222).
Epitopeab58841 recognizes an epitope between domains 2 and 5 of Mannose 6 Phosphate Receptor (Cation independent) (CD222).
- Jurkat whole cell lysate (ab7899)
The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use.
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We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.4
Preservative: 0.097% Sodium azide
Constituents: PBS, 0.2% BSA
Concentration information loading...
Purification notesThe purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Our Abpromise guarantee covers the use of ab58841 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 275 kDa. (non-reducing conditions).|
|Flow Cyt||Use 8.3µl for 106 cells.
ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
FunctionTransport of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. Lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6-phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex. This receptor also binds IGF2. Acts as a positive regulator of T-cell coactivation, by binding DPP4.
Sequence similaritiesBelongs to the MRL1/IGF2R family.
Contains 1 fibronectin type-II domain.
DomainContains 15 repeating units of approximately 147 AA harboring four disulfide bonds each. The most highly conserved region within the repeat consists of a stretch of 13 AA that contains cysteines at both ends.
Cellular localizationLysosome membrane. Colocalized with DPP4 in internalized cytoplasmic vesicles adjacent to the cell surface.
- Information by UniProt
- 300 kDa mannose 6 phosphate receptor antibody
- 300 kDa mannose 6-phosphate receptor antibody
- Cation independent mannose 6 phosphate receptor antibody
Lane 1 : FITC Anti-M6PR (cation independent) antibody [MEM-238] (ab58841)
Lane 2 : Mouse IgG1 isotype control
All lanes : Jurkat whole cell lysate
Predicted band size: 275 kDa
Overlay histogram showing HeLa cells stained with ab58841 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab58841, 1µg/1x106 cells) for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 (1µg/1x106 cells ). Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Tween used under the same conditions.
ab58841 has been referenced in 2 publications.
- Laube F Mannose-6-phosphate/insulin-like growth factor-II receptor in human melanoma cells: effect of ligands and antibodies on the receptor expression. Anticancer Res 29:1383-8 (2009). Inhibition Assay ; Human . PubMed: 19414391
- Leksa V et al. The N terminus of mannose 6-phosphate/insulin-like growth factor 2 receptor in regulation of fibrinolysis and cell migration. J Biol Chem 277:40575-82 (2002). WB, Flow Cyt ; Human . PubMed: 12189157