FITC Anti-MD2 antibody [18H10] (ab106105)

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Key features and details

  • FITC Mouse monoclonal [18H10] to MD2
  • Suitable for: Functional Studies, Flow Cyt
  • Reacts with: Human
  • Conjugation: FITC. Ex: 493nm, Em: 528nm
  • Isotype: IgG2b

Overview

  • Product name

    FITC Anti-MD2 antibody [18H10]
    See all MD2 primary antibodies

  • Description

    FITC Mouse monoclonal [18H10] to MD2

  • Host species

    Mouse

  • Conjugation

    FITC. Ex: 493nm, Em: 528nm

  • Specificity

    ab106105 reacts with MD2. However, it does not react with sMD2.

  • Tested applications

    Suitable for: Functional Studies, Flow Cytmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    TLR4/MD2 expressing CHO cells/ chimeric TLR4/MD2 fusion protein

  • General notes


    The monoclonal antibody 18H10 is able to inhibit bacterial binding to MD2.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    Constituents: 1% BSA, PBS
  • Concentration information loading...

  • Purity

    Protein G purified

  • Primary antibody notes

    The monoclonal antibody 18H10 is able to inhibit bacterial binding to MD2.

  • Clonality

    Monoclonal

  • Clone number

    18H10

  • Isotype

    IgG2b

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab106105 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

ab91368 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
Notes
Functional Studies
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

ab91368 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

Target

  • Function

    Cooperates with TLR4 in the innate immune response to bacterial lipopolysaccharide (LPS), and with TLR2 in the response to cell wall components from Gram-positive and Gram-negative bacteria. Enhances TLR4-dependent activation of NF-kappa-B. Cells expressing both MD2 and TLR4, but not TLR4 alone, respond to LPS.

  • Post-translational
    modifications

    N-glycosylated; high-mannose.

  • Cellular localization

    Secreted > extracellular space.
  • Information by UniProt

  • Database links

    • Alternative names

      • ESOP 1 antibody
      • ESOP-1 antibody
      • ESOP1 antibody
      • LY 96 antibody
      • Ly-96 antibody
      • LY96 antibody
      • LY96_HUMAN antibody
      • Lymphocyte antigen 96 antibody
      • md 2 antibody
      • MD 2 protein antibody
      • MD2 protein antibody
      • Myeloid differentiation protein 2 antibody
      • Protein MD 2 antibody
      • Protein MD-2 antibody
      • Protein MD2 antibody
      see all

    References (0)

    Publishing research using ab106105? Please let us know so that we can cite the reference in this datasheet.

    ab106105 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Question

    Could you provide the Flow cytometry image data please?

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    Answer

    Thank you for your enquiry.

    This antibody to MD-2, clone 18H10, has been extensively published. For flow cytometry data please refer to the 4 publications below.


    1. Pugin, J et al; Soluble MD-2 activity in plasma from patients with severe sepsis and septic shock. Blood 2004, 104: 4071
    [ [Figure 6, http://bloodjournal.hematologylibrary.org/content/104/13/4071.full.html]

    2. Daubeuf, B et al; TLR4/MD-2 monoclonal antibody therapy affords protection in experimental models of septic shock. J Immunol 2007, 179: 6107
    [Figure 2, http://www.jimmunol.org/content/179/9/6107.full.pdf]


    3. Elson, G et al; Contribution of Toll-like receptors to the innate immune response to Gram-negative and Gram-positive bacteria. Blood 2007, 109: 1574
    [Figure 2, http://bloodjournal.hematologylibrary.org/content/109/4/1574.full.pdf]

    4. Tissieres, P et al; Soluble MD-2 is an acute phase protein and an opsonin for Gram-negative bacteria. Blood 2008, 111: 2122
    [Figure 5, http://bloodjournal.hematologylibrary.org/content/111/4/2122.full]


    I hope this information will be helpful.

    Have a nice day!

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