FITC Anti-Methylated Lysine antibody (ab53527)
Key features and details
- FITC Rabbit polyclonal to Methylated Lysine
- Suitable for: ICC/IF
- Reacts with: Species independent
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG
Overview
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Product name
FITC Anti-Methylated Lysine antibody
See all Methylated Lysine primary antibodies -
Description
FITC Rabbit polyclonal to Methylated Lysine -
Host species
Rabbit -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Specificity
This antibody recognizes proteins methylated on lysine residues (mono and di-methylysine). It does not cross-react with acetylated proteins. Cross-reactivity to trimethyllysine has not been tested. -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Methylated protein [s] conjugated to KLH.
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Positive control
- Human melanoma cells (MMRU).
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General notes
FITC/Ab molar ratio: approximately 10:1
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
This antibody was affinity purified using N epsilon-monomethyllysine and dimethyllysine on Agarose as an affinity matrix. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab53527 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/50 - 1/100. |
Target
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Relevance
Many proteins are post translationally modified. Modifications such as phosphorylation, glycosylation, ubiquitination and methylation have been shown to play an important role in the development, physiology and disease of animals and plants. Histone lysine methylation is regarded as a very stable modification with important functions in epigenetic gene control and for organizing chromatin domains. -
Alternative names
- Dimethylysine antibody
- Methyl lysine antibody
- N epsilon dimethyl lysine antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - FITC Anti-Methylated Lysine antibody (ab53527)This image shows human melanoma (MMRU) cells stained with ab53527 at 1/50 dilution.
Protocols
Datasheets and documents
References (0)
ab53527 has not yet been referenced specifically in any publications.
