FITC Anti-Methylated Lysine antibody (ab53527)
Key features and details
- FITC Rabbit polyclonal to Methylated Lysine
- Suitable for: ICC/IF
- Reacts with: Species independent
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG
Overview
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Product name
FITC Anti-Methylated Lysine antibody
See all Methylated Lysine primary antibodies -
Description
FITC Rabbit polyclonal to Methylated Lysine -
Host species
Rabbit -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Specificity
This antibody recognizes proteins methylated on lysine residues (mono and di-methylysine). It does not cross-react with acetylated proteins. Cross-reactivity to trimethyllysine has not been tested. -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Chemical/ Small Molecule corresponding to Methylated Lysine conjugated to keyhole limpet haemocyanin. Methylated protein(s) conjugated to KLH.
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Positive control
- Human melanoma cells (MMRU).
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General notes
FITC/Ab molar ratio: approximately 10:1
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
This antibody was affinity purified using N epsilon-monomethyllysine and dimethyllysine on Agarose as an affinity matrix. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab53527 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/50 - 1/100.
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Notes |
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ICC/IF
1/50 - 1/100. |
Target
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Relevance
Many proteins are post translationally modified. Modifications such as phosphorylation, glycosylation, ubiquitination and methylation have been shown to play an important role in the development, physiology and disease of animals and plants. Histone lysine methylation is regarded as a very stable modification with important functions in epigenetic gene control and for organizing chromatin domains. -
Alternative names
- Dimethylysine antibody
- Methyl lysine antibody
- N epsilon dimethyl lysine antibody
see all
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab53527 has not yet been referenced specifically in any publications.