FITC Anti-MHC Class 1 H2 Db/H2-D1 antibody [27-11-13] (ab25028)
Key features and details
- FITC Mouse monoclonal [27-11-13] to MHC Class 1 H2 Db/H2-D1
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG2a
Related conjugates and formulations
Overview
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Product name
FITC Anti-MHC Class 1 H2 Db/H2-D1 antibody [27-11-13]
See all MHC Class 1 H2 Db/H2-D1 primary antibodies -
Description
FITC Mouse monoclonal [27-11-13] to MHC Class 1 H2 Db/H2-D1 -
Host species
Mouse -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Specificity
ab25028 recognizes with the a3 domain of H-2Db class I MHC (H2-D1) antigen. The antibody cross-reacts with the a3 domain of H-2Ld, H-2Dq and H-2Lq, but not H-2Kd or H-2Dd. Reactivity with haplotypes k, f, p, r, and s has not been observed.
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Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Tissue, cells or virus corresponding to Mouse MHC Class 1 H2 Db/H2-D1. BDF1 mouse splenocytes
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Epitope
An epitope on the a chain of H-2Db -
Positive control
- Flow cyt: Mouse C57BL/6 splenocytes.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Store In the Dark. -
Storage buffer
pH: 7.4
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
27-11-13 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab25028 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
Use 1µg for 106 cells.
ab18455 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
Notes |
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Flow Cyt
Use 1µg for 106 cells. ab18455 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
Target
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Relevance
In the mouse the MHC Class 1 loci are called H2K, H2L and H2D. The equivalent loci in the human MHC are HLA (Human Leukocyte Antigen). MHC class I molecules are heterodimers, consisting of a single transmembrane polypeptide chain (the a-chain) which is highly polymorphic, and the invariant ß2 microglobulin (which is encoded elsewhere, not in the MHC). MHC class I molecules are found on almost every nucleated cell of the body. Their major function is to present peptide fragments derived from antigens to cytotoxic T cells. -
Cellular localization
Cell Membrane; single pass type I membrane protein -
Database links
- Entrez Gene: 14964 Mouse
- SwissProt: P01899 Mouse
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Alternative names
- H 2D cell surface glycoprotein antibody
- H2 class I histocompatibility antigen, DB alpha chain antibody
- H2D(B) antibody
see all
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab25028 has not yet been referenced specifically in any publications.