FITC Anti-MHC class I antibody [34-1-2S] (ab95572)
![Flow Cytometry - FITC Anti-MHC class I antibody [34-1-2S] (ab95572)](https://www.abcam.com/ps/products/95/ab95572/Images/ab95572-109849-anti-mhc-class-i-antibody-34-1-2s-fitc-flow-cytometry.gif "Flow Cytometry - FITC Anti-MHC class I antibody [34-1-2S] (ab95572)")
Key features and details
- FITC Mouse monoclonal [34-1-2S] to MHC class I
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG2a
Overview
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Product name
FITC Anti-MHC class I antibody [34-1-2S]
See all MHC class I primary antibodies -
Description
FITC Mouse monoclonal [34-1-2S] to MHC class I -
Host species
Mouse -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Specificity
ab95572 reacts with the mouse MHC class I, H-2Kd and H-2Dd. This cytotoxic antibody also cross reacts with K b,s,r,q,p -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
C3H.SW Mouse splenocytes
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Positive control
- Mouse splenocytes
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.87% Sodium chloride, PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
34-1-2S -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab95572 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use 1µg for 105-8 cells. Staining the cell sample in a final volume of 100 µL is recommended.
ab18455 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|
Target
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Relevance
MHC Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. MHC class I antigens are heterodimers consisting of one alpha chain (44kDa) with beta 2 microglobulin (11.5 kDa). The antigen is expressed by all somatic cells at varying levels. MHC Class I molecules are expressed on most nucleated cells where they present endogenously synthesized antigenic peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. Fibroblasts or neurons however only show a low level of antigen. -
Cellular localization
Cell Membrane; Type I membrane protein. -
Alternative names
- A 28 antibody
- A 9 antibody
- Antigen presenting molecule antibody
see all
Images
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Flow Cytometry - FITC Anti-MHC class I antibody [34-1-2S] (ab95572)Flow cytometric analysis of Mouse splenocytes labelled with ab95572 at 1 µg/test (blue histogram) or with an isotype control (open histogram).
Datasheets and documents
References (0)
ab95572 has not yet been referenced specifically in any publications.
