FITC Anti-MHC class II I E kappa antibody [14-4-4S] (ab25023)

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Flow Cytometry - FITC Anti-MHC class II I E kappa antibody [14-4-4S] (ab25023)

    Key features and details

    • FITC Mouse monoclonal [14-4-4S] to MHC class II I E kappa
    • Suitable for: Flow Cyt
    • Reacts with: Mouse
    • Conjugation: FITC. Ex: 493nm, Em: 528nm
    • Isotype: IgG2a

    Overview

    • Product name

      FITC Anti-MHC class II I E kappa antibody [14-4-4S]
      See all MHC class II I E kappa primary antibodies

    • Description

      FITC Mouse monoclonal [14-4-4S] to MHC class II I E kappa

    • Host species

      Mouse

    • Conjugation

      FITC. Ex: 493nm, Em: 528nm

    • Specificity

      ab25023 recognises MHC class II I E kappa alloantigen on cells from mice of the H-2d, H-2p, and H-2r haplotypes. It cross reacts with alloantigen RT1D. Cells from mice of the H-2b, H-2f, H-2q, and H-2s haplotypes do not express this antigen.

    • Tested applications

      Suitable for: Flow Cytmore details

    • Species reactivity

      Reacts with: Mouse

    • Immunogen

      Tissue/ cell preparation: C3H mouse skin graft and splenocytes.

    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Store at +4°C.

    • Storage buffer

      pH: 7.4
      Preservative: 0.1% Sodium azide
      Constituent: PBS
    • Concentration information loading...

    • Purity

      Affinity purified

    • Clonality

      Monoclonal

    • Clone number

      14-4-4S

    • Isotype

      IgG2a

    • Light chain type

      kappa

    • Research areas

    Applications

    Our Abpromise guarantee covers the use of ab25023 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt
  • Application notes
    BL: Use at an assay dependent dilution for in vitro blocking of antigen presentation.
    Flow Cyt: Use 1µg for 106 cells.
    FuncS: Use at an assay dependent dilution for complement dependent cytotoxicity studies and in vitro induction of antigen specific B cell differentiation.
    IHC-Fr: Use at an assay dependent dilution.
    IP: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • Relevance

      A major histocompatibility complex class II receptor. These display processed antigens from virally infected or transformed cells. Class II positive cells ('antigen presenting cells') can take up antigens from outside by endocytosis, degrade them into small peptides, and re export the peptides (now bound to MHC class II protein) to the cell surface. These peptide MHC class II complexes can then be recognized by specific CD4+ lymphocytes.

    • Cellular localization

      Type I membrane protein

    • Alternative names

      • H2 Ea antibody
      • H2-Ea antibody

    Images

    • Flow Cytometry - FITC Anti-MHC class II I E kappa antibody [14-4-4S] (ab25023)
      Flow Cytometry - FITC Anti-MHC class II I E kappa antibody [14-4-4S] (ab25023)

      Flow cytometry analysis staining MHC class II I E kappa in mouse splenocytes using ab25023 at a dilution of 1 ug/106 cells.

    References (1)

    Publishing research using ab25023? Please let us know so that we can cite the reference in this datasheet.

    ab25023 has been referenced in 1 publication.

    • Guan J  et al. Enhanced immunocompatibility of ligand-targeted liposomes by attenuating natural IgM absorption. Nat Commun 9:2982 (2018). PubMed: 30061672

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