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    fitc-mouse-igg1-b116-isotype-control-ab91356.pdf

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Isotype/Loading Controls Isotype Controls Mouse
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FITC Mouse IgG1 [B11/6] - Isotype Control (ab91356)

  • Datasheet
  • SDS
Q&A (3)References (8)

Product price, shipping and contact information

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Overview

  • Product name

    FITC Mouse IgG1 [B11/6] - Isotype Control
    See all Mouse isotype controls
  • Conjugation

    FITC. Ex: 493nm, Em: 528nm
  • Specificity

    ab91356 was produced against a synthetic hapten, which is normally not present in Humans or animals. Therefore, this mouse IgG1 does not react with any antigen of Human or animal cells.
  • Tested applications

    Suitable for: Flow Cytmore details
  • Immunogen

    Vegetal protein

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Store In the Dark.
  • Storage buffer

    pH: 7.20
    Preservative: 0.1% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    B11/6
  • Isotype

    IgG1
  • Research areas

    • Isotype/Loading Controls
    • Isotype Controls
    • Mouse
  • Alternative names

    • Mouse Isotype Control

Associated products

  • Alternative Versions

    • PerCP Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab118658)
    • CF405M Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab126026)
    • Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)
    • PE Mouse IgG1 [B11/6] - Isotype Control (ab91357)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)

Applications

Our Abpromise guarantee covers the use of ab91356 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

ab91356 is a negative isotype control.

Protocols

  • Flow cytometry protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (8)

    Publishing research using ab91356? Please let us know so that we can cite the reference in this datasheet.

    ab91356 has been referenced in 8 publications.

    • Chamberlain N  et al. Lung epithelial protein disulfide isomerase A3 (PDIA3) plays an important role in influenza infection, inflammation, and airway mechanics. Redox Biol 22:101129 (2019). PubMed: 30735910
    • Ode Y  et al. Cold-inducible RNA-binding Protein Induces Neutrophil Extracellular Traps in the Lungs during Sepsis. Sci Rep 9:6252 (2019). PubMed: 31000768
    • Zhang L  et al. Hypoxia preconditioned renal tubular epithelial cell-derived extracellular vesicles alleviate renal ischaemia-reperfusion injury mediated by the HIF-1a/Rab22 pathway and potentially affected by microRNAs. Int J Biol Sci 15:1161-1176 (2019). PubMed: 31223277
    • Khan MA  et al. C5a Blockade Increases Regulatory T Cell Numbers and Protects Against Microvascular Loss and Epithelial Damage in Mouse Airway Allografts. Front Immunol 9:1010 (2018). PubMed: 29881374
    • Tomlinson JE  et al. Multispectral fluorescence-activated cell sorting of B and T cell subpopulations from equine peripheral blood. Vet Immunol Immunopathol 199:22-31 (2018). PubMed: 29678226
    • Liu H  et al. Human umbilical vein endothelial cell vaccine suppresses the angiogenesis of esophageal squamous cell carcinoma in a humanized mouse model. Oncol Rep 40:3006-3014 (2018). PubMed: 30132560
    • Sun W  et al. Proanthocyanidins Attenuation of H2O2-Induced Oxidative Damage in Tendon-Derived Stem Cells via Upregulating Nrf-2 Signaling Pathway. Biomed Res Int 2017:7529104 (2017). PubMed: 29201913
    • Lau WH  et al. Combinatorial treatment with polyI:C and anti-IL6 enhances apoptosis and suppresses metastasis of lung cancer cells. Oncotarget 8:32884-32904 (2017). PubMed: 28427199

    Customer reviews and Q&As

    Q&A
    Submit a question

    1-3 of 3 Q&A

    Question

    Could you tell me the volume per vial of the isotype control ab91356? On your website it is only stated “50 tests”. Could you confirm the antibody concentration of 0.05 mg/ml as well?

    Read More

    Abcam community

    Verified customer

    Asked on Jan 30 2018

    Answer

    The volume per vial of ab91356 is 1 ml. It is possible to perform 50 tests, as 20 µl is required per test.
    I also confirm the concentration of our current vials in stock are at 0.05 mg/ml.

    Read More

    Abcam Scientific Support

    Answered on Jan 30 2018

    Question

    What is the concentration of lot GR65536?

    Read More

    Abcam community

    Verified customer

    Asked on Jul 24 2012

    Answer

    Thank youvery much for your call yesterday and for your patience while I have looked into your enquiry.



    I've heard back from the lab, and the concentration of this product is 0.025 mg/ml.



    I hope this information will be useful, but if you have further questions or if there is anything else that we can do for you, please let me know and I'll be happy to help.

    Read More

    Abcam Scientific Support

    Answered on Jul 24 2012

    Question

    Dear Sir/ Madam
    Please check the below quiry and help us.
    "I am planning to use Anti - CD18 antibody [CLB-LFA-1/1,54] (FITC) (ab51573) antibody for my work, if i have to use a negative control, will this isotype control - Mouse IgG1 [ICIG1] – Isotype Control (ab91356) work well with it? is it compatible as reference for Anti - CD18 antibody [CLB-LFA-1/1,54] (FITC) (ab51573). If not what alternative isotype control should i go for.?"
    Regards

    Read More

    Abcam community

    Verified customer

    Asked on Feb 19 2013

    Answer

    Thank you for contacting us.

    I can confirm that ab91356 (Mouse IgG1 [ICIG1] (FITC) – Isotype Control) can be used as a negative isotype control for ab51573 (Anti-CD18 antibody [CLB-LFA-1/1, 54] (FITC)) with Flow Cytometry.

    I hope this helps. Please let us know if you know further assistance.

    Read More

    Abcam Scientific Support

    Answered on Feb 19 2013

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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