FITC Mouse IgG1, monoclonal [HybIgG1]  - Isotype Control (ab1264)

Q&A (3)

Overview

  • Product name

    FITC Mouse IgG1, monoclonal [HybIgG1]  - Isotype Control
    See all Mouse isotype controls

  • Description

    FITC Mouse IgG1, monoclonal [HybIgG1] - Isotype Control

  • Conjugation

    FITC. Ex: 493nm, Em: 528nm

  • Tested applications

    Suitable for: Flow Cytmore details

  • General notes

    This antibody is a direct immunofluorescence reagent used as an isotype (negative) control for flow cytometric immunophenotyping of erythrocyte-lysed whole blood with monoclonal antibodies.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    Preservative: 0.1% Sodium azide
    Constituent: 0.5% BSA
  • Concentration information loading...

  • Isotype control notes

    This antibody is a direct immunofluorescence reagent used as an isotype (negative) control for flow cytometric immunophenotyping of erythrocyte-lysed whole blood with monoclonal antibodies.

  • Clonality

    Monoclonal

  • Clone number

    HybIgG1

  • Myeloma

    unknown

  • Isotype

    IgG1

  • Light chain type

    unknown

  • Research areas

  • Alternative names

    • Mouse Isotype Control

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab1264 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
Use at an assay dependent concentration. This antibody is used to determine the unstained lymphocyte population and to determine any non-antigen-specific antibody binding on mononuclear cells separated by density gradient or on lysed whole blood. IgG1 (FITC) immunofluorescence analysis can be performed on a flow cytometer equipped with an excitation source of 488nm and fitted with logarithmic amplifiers. 10µl ofIgG1 (FITC) is sufficient for labelling of 1x106 cells. Molecular F/P ratio: 3.0-6.0
Notes
Flow Cyt
Use at an assay dependent concentration. This antibody is used to determine the unstained lymphocyte population and to determine any non-antigen-specific antibody binding on mononuclear cells separated by density gradient or on lysed whole blood. IgG1 (FITC) immunofluorescence analysis can be performed on a flow cytometer equipped with an excitation source of 488nm and fitted with logarithmic amplifiers. 10µl ofIgG1 (FITC) is sufficient for labelling of 1x106 cells. Molecular F/P ratio: 3.0-6.0

References (0)

Publishing research using ab1264? Please let us know so that we can cite the reference in this datasheet.

ab1264 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Q&A
Submit a question

1-3 of 3 Q&A

Question

Testing discount codes for ab105439 & ab97715 to test in flowcytometry.

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Answer

DISCOUNT CODE: *********- For ab105439
DISCOUNT CODE: *******- For ab97715
Expiration date: *************

I am very pleased to hear you would like to accept our offer and test ab105439 & ab97715in Flow Cytometry. This code will give you: 1 free PRIMARY ANTIBODY per discount code before the expiration date. To redeem this offer, please submit an Abreview forFlow Cytometryand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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Question

We spoke over the telephone regarding product code ab1286 which is a mixture of two clones: 1) Isotype: IgG1, Clone:HybIgG1, Label:FITC 2) Isotype: IgG2a, Clone:HybIgG2a, Label:PE Ideally I want just clone HybIgG1 labelled with FITC. Do you stock this product?

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Answer

I think ab1264 is what you are looking for!

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Question

Is this antibody a suitable negative control for ICC applications?

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Answer

Thank you for your enquiry. To our knowledge, this antibody has yet to be tested in ICC. All tested applications are specified on Abcam product datasheets. If you decide to go ahead and purchase this product, please let us know how you get on and in return we will forward a reward of your choice, typically an Amazon gift voucher.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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