For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    fitc-mouse-igg1-monoclonal-hybigg1-isotype-control-ab1264.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Isotype/Loading Controls Isotype Controls Mouse
Share by email

FITC Mouse IgG1, monoclonal [HybIgG1]  - Isotype Control (ab1264)

  • Datasheet
  • SDS
Q&A (3)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

You may also be interested in

Conjugation
Product image
FITC Conjugation Kit - Lightning-Link® (ab102884)
Primary
FITC Anti-CD19 antibody [CB19] (ab1167)
Primary
Product image
Anti-CD34 antibody [4H11(APG)] (ab762)

View more associated products

Overview

  • Product name

    FITC Mouse IgG1, monoclonal [HybIgG1]  - Isotype Control
    See all Mouse isotype controls
  • Description

    FITC Mouse IgG1, monoclonal [HybIgG1] - Isotype Control
  • Conjugation

    FITC. Ex: 493nm, Em: 528nm
  • Tested applications

    Suitable for: Flow Cytmore details
  • General notes

    This antibody is a direct immunofluorescence reagent used as an isotype (negative) control for flow cytometric immunophenotyping of erythrocyte-lysed whole blood with monoclonal antibodies.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.1% Sodium azide
    Constituent: 0.5% BSA
  • Concentration information loading...
  • Isotype control notes

    This antibody is a direct immunofluorescence reagent used as an isotype (negative) control for flow cytometric immunophenotyping of erythrocyte-lysed whole blood with monoclonal antibodies.
  • Clonality

    Monoclonal
  • Clone number

    HybIgG1
  • Myeloma

    unknown
  • Isotype

    IgG1
  • Light chain type

    unknown
  • Research areas

    • Isotype/Loading Controls
    • Isotype Controls
    • Mouse
  • Alternative names

    • Mouse Isotype Control

Associated products

  • Alternative Versions

    • Dual color: FITC + PE Mouse IgG1monoclonal [HybIgG1] - isotype control (ab1285)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab1264 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
Use at an assay dependent concentration. This antibody is used to determine the unstained lymphocyte population and to determine any non-antigen-specific antibody binding on mononuclear cells separated by density gradient or on lysed whole blood. IgG1 (FITC) immunofluorescence analysis can be performed on a flow cytometer equipped with an excitation source of 488nm and fitted with logarithmic amplifiers. 10µl ofIgG1 (FITC) is sufficient for labelling of 1x106 cells. Molecular F/P ratio: 3.0-6.0
Notes
Flow Cyt
Use at an assay dependent concentration. This antibody is used to determine the unstained lymphocyte population and to determine any non-antigen-specific antibody binding on mononuclear cells separated by density gradient or on lysed whole blood. IgG1 (FITC) immunofluorescence analysis can be performed on a flow cytometer equipped with an excitation source of 488nm and fitted with logarithmic amplifiers. 10µl ofIgG1 (FITC) is sufficient for labelling of 1x106 cells. Molecular F/P ratio: 3.0-6.0

Protocols

  • Flow cytometry protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (0)

    Publishing research using ab1264? Please let us know so that we can cite the reference in this datasheet.

    ab1264 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Q&A
    Submit a question

    1-3 of 3 Q&A

    Question

    Testing discount codes for ab105439 & ab97715 to test in flowcytometry.

    Read More

    Abcam community

    Verified customer

    Asked on Jun 05 2012

    Answer

    DISCOUNT CODE: *********- For ab105439
    DISCOUNT CODE: *******- For ab97715
    Expiration date: *************

    I am very pleased to hear you would like to accept our offer and test ab105439 & ab97715in Flow Cytometry. This code will give you: 1 free PRIMARY ANTIBODY per discount code before the expiration date. To redeem this offer, please submit an Abreview forFlow Cytometryand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
    For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


    Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

    Read More

    Abcam Scientific Support

    Answered on Jun 05 2012

    Question

    We spoke over the telephone regarding product code ab1286 which is a mixture of two clones: 1) Isotype: IgG1, Clone:HybIgG1, Label:FITC 2) Isotype: IgG2a, Clone:HybIgG2a, Label:PE Ideally I want just clone HybIgG1 labelled with FITC. Do you stock this product?

    Read More

    Abcam community

    Verified customer

    Asked on Jun 22 2004

    Answer

    I think ab1264 is what you are looking for!

    Read More

    Abcam Scientific Support

    Answered on Jun 22 2004

    Question

    Is this antibody a suitable negative control for ICC applications?

    Read More

    Abcam community

    Verified customer

    Asked on Oct 27 2003

    Answer

    Thank you for your enquiry. To our knowledge, this antibody has yet to be tested in ICC. All tested applications are specified on Abcam product datasheets. If you decide to go ahead and purchase this product, please let us know how you get on and in return we will forward a reward of your choice, typically an Amazon gift voucher.

    Read More

    Abcam Scientific Support

    Answered on Oct 27 2003

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2021 Abcam plc. All rights reserved.