FITC Mouse IgG2a [X5563] - Isotype Control (ab91362)

Q&A (3)References (2)

Overview

  • Product name

    FITC Mouse IgG2a [X5563] - Isotype Control
    See all Mouse isotype controls

  • Conjugation

    FITC. Ex: 493nm, Em: 528nm

  • Tested applications

    Suitable for: Flow Cytmore details

  • Immunogen

    Vegetal protein

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Store In the Dark.

  • Storage buffer

    pH: 7.20
    Preservative: 0.1% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...

  • Purity

    Protein G purified

  • Clonality

    Monoclonal

  • Clone number

    X5563

  • Isotype

    IgG2a

  • Research areas

  • Alternative names

    • Mouse Isotype Control

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab91362 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
Notes
Application notes
Flow Cyt: Use 20µl for 106 cells.
ab91362 is a negative isotype control


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

References (2)

Publishing research using ab91362? Please let us know so that we can cite the reference in this datasheet.

ab91362 has been referenced in 2 publications.

  • Ligorio M  et al. Stromal Microenvironment Shapes the Intratumoral Architecture of Pancreatic Cancer. Cell 178:160-175.e27 (2019). PubMed: 31155233
  • Yarzabek B  et al. Variations in HLA-B cell surface expression, half-life and extracellular antigen receptivity. Elife 7:N/A (2018). PubMed: 29989547

Customer reviews and Q&As

Q&A
Submit a question

1-3 of 3 Q&A

Question

Please provide the concentrations of the following isotypes controls:



AB91362

AB91363

AB91541

AB91539

AB37347

AB91546

AB1284

AB91368

AB118540



Thanks in advance.

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Answer

Thank you for contacting us.
Do you have the lot. numbers at hand or is this rather a enquiry about the lot. numbers we have have in stock?
Looking forward to your reply.

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Question

What is the concentration of the isotype control ab91362?

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Answer

Thank you for contacting us.

The concentration of lot GR30599 of this product is 0.0125 mg/ml.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question

Dear Colleagues, My customer is interested in Anti-Hepatitis C Virus NS4 antibody [1G7] (FITC) (ab2592). She would like to know: 1)      which isotype control do you recommend for these antibodies? 2)      and which way of permabilization do you recommend? I would be grateful for your help.   Best regards,    

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Answer

Thank you for your enquiry and your interest in our products. You can advise your customer to use our advanced search engine for control at this site: https://www.abcam.com/index.html?c=718 The isotype of this antibody is IgG2a and the host species is mouse. I have done a search for you and found ab91362 which may suit your customer need. We have some useful information at our website regarding  different permeabilization steps, please draw recommend your customer: https://www.abcam.com/index.html?pageconfig=resource&rid=11448 I hope this helps and if I can assist further, please do not hesitate to contact me.  

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