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  1. Link

    fitc-mouse-igg2a-x5563-isotype-control-ab91362.pdf

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Isotype/Loading Controls Isotype Controls Mouse
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FITC Mouse IgG2a [X5563] - Isotype Control (ab91362)

  • Datasheet
  • SDS
Q&A (3)References (1)

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Abpromise

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Overview

  • Product name

    FITC Mouse IgG2a [X5563] - Isotype Control
    See all Mouse isotype controls
  • Conjugation

    FITC. Ex: 493nm, Em: 528nm
  • Tested applications

    Suitable for: Flow Cytmore details
  • Immunogen

    Vegetal protein

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Store In the Dark.
  • Storage buffer

    pH: 7.20
    Preservative: 0.1% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    X5563
  • Isotype

    IgG2a
  • Research areas

    • Isotype/Loading Controls
    • Isotype Controls
    • Mouse
  • Alternative names

    • Mouse Isotype Control

Associated products

  • Alternative Versions

    • PE Mouse IgG2a [X5563] - Isotype Control (ab91363)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)

Applications

Our Abpromise guarantee covers the use of ab91362 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
  • Application notes
    Flow Cyt: Use 20µl for 106 cells.
    ab91362 is a negative isotype control


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Protocols

    • Flow cytometry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (1)

    Publishing research using ab91362? Please let us know so that we can cite the reference in this datasheet.

    ab91362 has been referenced in 1 publication.

    • Yarzabek B  et al. Variations in HLA-B cell surface expression, half-life and extracellular antigen receptivity. Elife 7:N/A (2018). PubMed: 29989547

    Customer reviews and Q&As

    Q&A
    Submit a question

    1-3 of 3 Q&A

    Question

    Please provide the concentrations of the following isotypes controls:



    AB91362

    AB91363

    AB91541

    AB91539

    AB37347

    AB91546

    AB1284

    AB91368

    AB118540



    Thanks in advance.

    Read More

    Abcam community

    Verified customer

    Asked on Sep 11 2012

    Answer

    Thank you for contacting us.
    Do you have the lot. numbers at hand or is this rather a enquiry about the lot. numbers we have have in stock?
    Looking forward to your reply.

    Read More

    Abcam Scientific Support

    Answered on Sep 11 2012

    Question

    What is the concentration of the isotype control ab91362?

    Read More

    Abcam community

    Verified customer

    Asked on Jul 11 2012

    Answer

    Thank you for contacting us.

    The concentration of lot GR30599 of this product is 0.0125 mg/ml.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on Jul 11 2012

    Question

    Dear Colleagues, My customer is interested in Anti-Hepatitis C Virus NS4 antibody [1G7] (FITC) (ab2592). She would like to know: 1)      which isotype control do you recommend for these antibodies? 2)      and which way of permabilization do you recommend? I would be grateful for your help.   Best regards,    

    Read More

    Abcam community

    Verified customer

    Asked on Nov 04 2011

    Answer

    Thank you for your enquiry and your interest in our products. You can advise your customer to use our advanced search engine for control at this site: https://www.abcam.com/index.html?c=718 The isotype of this antibody is IgG2a and the host species is mouse. I have done a search for you and found ab91362 which may suit your customer need. We have some useful information at our website regarding  different permeabilization steps, please draw recommend your customer: https://www.abcam.com/index.html?pageconfig=resource&rid=11448 I hope this helps and if I can assist further, please do not hesitate to contact me.  

    Read More

    Abcam Scientific Support

    Answered on Nov 04 2011

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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