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  1. Link

    fitc-myc-tag-antibody-ab1263.pdf

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Tags & Cell Markers Epitope Tags Myc Tag
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FITC Anti-Myc tag antibody (ab1263)

  • Datasheet
  • SDS
Reviews (1)Q&A (2)References (6)

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Immunocytochemistry/ Immunofluorescence - FITC Anti-Myc tag antibody (ab1263)

    Key features and details

    • FITC Goat polyclonal to Myc tag
    • Suitable for: ICC, ICC/IF
    • Conjugation: FITC. Ex: 493nm, Em: 528nm
    • Isotype: IgG

    Overview

    • Product name

      FITC Anti-Myc tag antibody
      See all Myc tag primary antibodies
    • Description

      FITC Goat polyclonal to Myc tag
    • Host species

      Goat
    • Conjugation

      FITC. Ex: 493nm, Em: 528nm
    • Tested applications

      Suitable for: ICC, ICC/IFmore details
    • Immunogen

      Full length native protein

      EQKLISEEDL

      (c-myc) (purified): conjugated to KLH.
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • General notes

      F/P ratio is 3 to 7.

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C.
    • Storage buffer

      Preservative: 0.1% Sodium azide
      Constituent: 0.2% BSA
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Purification notes

      Antibodies were affinity purified using the peptide immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Tags & Cell Markers
      • Epitope Tags
      • Myc Tag

    Associated products

    • Immunizing Peptide (Blocking)

      • Human c-Myc peptide (ab13837)
    • Isotype control

      • FITC Goat IgG - Isotype control (ab37374)

    Applications

    Our Abpromise guarantee covers the use of ab1263 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ICC 1/200 - 1/2000.
    ICC/IF 1/200. (see Abreview).

    Target

    • Relevance

      Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.
    • Cellular localization

      Nuclear
    • Alternative names

      • c-myc tag antibody
      • Myc Epitope Tag antibody

    Images

    • Immunocytochemistry/ Immunofluorescence - FITC Anti-Myc tag antibody (ab1263)
      Immunocytochemistry/ Immunofluorescence - FITC Anti-Myc tag antibody (ab1263)Image from Poncet D et al, J Cell Biol. 2006 Sep 25;174(7):985-96. Epub 2006 Sep 18, Fig 3D. DOI 10.1083/jcb.200604069
      ab1263 staining Myc tag (red) in HeLa cells by Immunocytochemistry/ Immunofluorescence.

    Protocols

    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (6)

    Publishing research using ab1263? Please let us know so that we can cite the reference in this datasheet.

    ab1263 has been referenced in 6 publications.

    • Baud C  et al. Translocation path of a substrate protein through its Omp85 transporter. Nat Commun 5:5271 (2014). PubMed: 25327833
    • Zou S  et al. A novel actin-binding domain on Slo1 calcium-activated potassium channels is necessary for their expression in the plasma membrane. Mol Pharmacol 73:359-68 (2008). PubMed: 17989352
    • Zou S  et al. The beta 1 subunit of L-type voltage-gated Ca2+ channels independently binds to and inhibits the gating of large-conductance Ca2+-activated K+ channels. Mol Pharmacol 73:369-78 (2008). PubMed: 17989350
    • Kim EY  et al. Nephrin binds to the COOH terminus of a large-conductance Ca2+-activated K+ channel isoform and regulates its expression on the cell surface. Am J Physiol Renal Physiol 295:F235-46 (2008). ICC/IF . PubMed: 18480178
    • Kim EY  et al. Interactions with filamin A stimulate surface expression of large-conductance Ca2+-activated K+ channels in the absence of direct actin binding. Mol Pharmacol 72:622-30 (2007). PubMed: 17586600
    • Poncet D  et al. Cytopathic effects of the cytomegalovirus-encoded apoptosis inhibitory protein vMIA. J Cell Biol 174:985-96 (2006). PubMed: 16982800

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-3 of 3 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Myc tag antibody (FITC)

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rat Cell (Mesenchymal stem cell)
    Specification
    Mesenchymal stem cell
    Fixative
    Paraformaldehyde
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2%
    Read More

    Dr. Mal Niladri

    Verified customer

    Submitted Nov 08 2006

    Question

    We are using ab1263 [anti-Myc, FITC-conjugated] extensively [and many other Abcam products, including custom Ab prep.] For our use in FRET, we need to know the affinity value of ab1263 for its peptide.Can you give me that value ,please? [That is not covered in the datasheet or technical enquiry page for 1263].

    Read More

    Abcam community

    Verified customer

    Asked on Nov 05 2004

    Answer

    Unfortunately we do not have that information.

    Read More

    Abcam Scientific Support

    Answered on Nov 05 2004

    Question

    hi - I have a question about the myc tag antibody, catalogue number ab1263. I it FITC conjugated - but you say it can be used in Immunohistochemistry - can it be used using the traditional ABC method? or just immunofluorescence? Thanks,

    Read More

    Abcam community

    Verified customer

    Asked on Nov 27 2003

    Answer

    We suggest that the FITC conjugated antibody should be used for immunofluorescence.

    Read More

    Abcam Scientific Support

    Answered on Dec 02 2003

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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