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    fitc-myc-tag-antibody-ab1393.pdf

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Tags & Cell Markers Epitope Tags Myc Tag
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FITC Anti-Myc tag antibody (ab1393)

  • Datasheet
  • SDS
Reviews (1)Q&A (1)References (3)

Product price, shipping and contact information

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Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Key features and details

  • FITC Rabbit polyclonal to Myc tag
  • Suitable for: WB, ELISA
  • Conjugation: FITC. Ex: 493nm, Em: 528nm
  • Isotype: IgG

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Conjugation
Product image
FITC Conjugation Kit - Lightning-Link® (ab102884)

View more associated products

Overview

  • Product name

    FITC Anti-Myc tag antibody
    See all Myc tag primary antibodies
  • Description

    FITC Rabbit polyclonal to Myc tag
  • Host species

    Rabbit
  • Conjugation

    FITC. Ex: 493nm, Em: 528nm
  • Tested applications

    Suitable for: WB, ELISAmore details
  • Immunogen

    EQKLISEEDL (C-MYC) conjugated with KLH.

  • General notes

    Molar F/P ratio is 4.6:1

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.19% HEPES, 0.58% Sodium chloride, 0.2% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Antibodies were immunoaffinity purified using the peptide conjugated to a solid-phase support and conjugated to fluorescein isothiocyanate.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Epitope Tags
    • Myc Tag

Associated products

  • Immunizing Peptide (Blocking)

    • Human c-Myc peptide (ab13837)
  • Isotype control

    • FITC-Rabbit IgG - Isotype Control (ab37406)

Applications

Our Abpromise guarantee covers the use of ab1393 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Can be blocked with Human c-Myc peptide (ab13837).
ELISA 1/1000.

Target

  • Relevance

    Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.
  • Cellular localization

    Nuclear
  • Alternative names

    • c-myc tag antibody
    • Myc Epitope Tag antibody

Protocols

  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (3)

    Publishing research using ab1393? Please let us know so that we can cite the reference in this datasheet.

    ab1393 has been referenced in 3 publications.

    • Najjar F  et al. Sex differences in depression-like behavior and neuroinflammation in rats post-MI: role of estrogens. Am J Physiol Heart Circ Physiol 315:H1159-H1173 (2018). PubMed: 30052050
    • Jain M  et al. Linear assembly of a human centromere on the Y chromosome. Nat Biotechnol 36:321-323 (2018). PubMed: 29553574
    • Xiao C  et al. Targeting HSP70 to motoneurons protects locomotor activity from hyperthermia in Drosophila. Dev Neurobiol 67:438-55 (2007). PubMed: 17443800

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    Flow Cytometry abreview for Anti-Myc tag antibody (FITC)

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Saccharomyces cerevisiae Cell (Yeast cells)
    Permeabilization
    No
    Gating Strategy
    Cells that were Myc-FITC positive
    Specification
    Yeast cells
    Preparation
    Cell harvesting/tissue preparation method: Harvested them in standard PBS with 0.1% BSA
    Sample buffer: PBS with 0.1% BSA
    Fixation
    No fixiation
    Read More

    Dr. Kaushik Thakkar

    Verified customer

    Submitted Jul 03 2019

    Question

    To the attention of: Abcam, Technical support
    Concerns : ab1393, rabbit polyclonal to MYC tag, FITC
    Dear Mrs, Mr,
    I searched the Abcam website about this product. There is one scientific reference, but the application is very specific and does not help us.
    Do you have more information to use this product for immunofluorescence on cultured cells ? (blocking solution, incubation time,…)
    Sincerely yours,

    Read More

    Abcam community

    Verified customer

    Asked on Dec 05 2012

    Answer

    Thank you very much for your interest in ab1393.

    Unfortunately we do not have more information about the use of the anti-Myc tag antibody (FITC) ab1393 in ICC/IF than the one available in the publication (PubMed: 17443800) and we do not currently have an image.

    However by participating in our AbTrial program you can now use our products in an untested application or species without financial risk.

    Simply follow these easy steps below to apply for our AbTrial Program:
    1. Reply to this email, letting us know you are interested in testing this product.
    2. Our scientists will email you an inactive personal discount code for the value of the product.
    3. Purchase and test the product at the regular price.
    4. Submit an image of your results, including your discount code in the additional notes section of your Abreview.
    5. Once the Abreview is submitted, the discount code will become active.
    6. Apply your discount code on your next order to receive that value off.

    Please let me know if you have any questions about this offer and I would be happy to help you further.

    The Terms and Conditions of this offer can be found at: https://www.abcam.com/abtrial.

    About the ICC/IF protocol, we recommend the following procedure: https://www.abcam.com/index.html?pageconfig=resource&rid=11417.

    Read More

    Abcam Scientific Support

    Answered on Dec 05 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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