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  1. Link

    fitc-mycobacterium-tuberculosis-antibody-ab20962.pdf

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Microbiology Organism Bacteria Gram positive bacteria Mycobacterium
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FITC Anti-Mycobacterium tuberculosis antibody (ab20962)

  • Datasheet
  • SDS
Submit a review Q&A (3)References (4)

Product price, shipping and contact information

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Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Key features and details

  • FITC Rabbit polyclonal to Mycobacterium tuberculosis
  • Suitable for: IHC-P, ICC/IF
  • Conjugation: FITC. Ex: 493nm, Em: 528nm
  • Isotype: IgG

Overview

  • Product name

    FITC Anti-Mycobacterium tuberculosis antibody
    See all Mycobacterium tuberculosis primary antibodies
  • Description

    FITC Rabbit polyclonal to Mycobacterium tuberculosis
  • Host species

    Rabbit
  • Conjugation

    FITC. Ex: 493nm, Em: 528nm
  • Tested applications

    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity

  • Immunogen

    Purified PPD.

  • General notes

    The antibody is covalently coupled with high purity Isomer I of fluorescein isothiocyanate. Care is taken to ensure complete removal of any free fluorescein from the final product.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.1% Sodium azide
    Constituents: 0.0268% PBS, 1% BSA
  • Concentration information loading...
  • Purity

    IgG fraction
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Microbiology
    • Organism
    • Bacteria
    • Gram positive bacteria
    • Mycobacterium

Associated products

  • Isotype control

    • FITC-Rabbit IgG - Isotype Control (ab37406)

Applications

Our Abpromise guarantee covers the use of ab20962 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/10 - 1/50.
ICC/IF Use at an assay dependent dilution. Acetone fixation is recommended.

Target

  • Relevance

    Mycobacterium tuberculosis is the most common cause of tuberculosis. Primary infection begins with inhalation of 1 to 10 aerosolised bacilli. The pathogenicity of the organism is determined by its ability to escape host immune responses as well as eliciting delayed hypersensitivity. Alveolar macrophages engulf the invading cells but are unable to mount an effective defense. Several virulence factors are responsible for this apparent failure; most notably in the mycobacterial cell wall are the cord factor, lipoarabinomannan, and the 65 kd heat shock protein or HSP65. The emergence of new strains of resistant Mycobacterium tuberculosis has created new interest in clinical diagnosis. Studies have shown immunohistochemical techniques to be superior to conventional special stains. Thus the demonstration of mycobacterial antigens are not only useful in establishing mycobacterial aetiology, but can also be used as an alternative method to the conventional Ziehl-Neelsen method.
  • Alternative names

    • M. tuberculosis antibody

Protocols

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (4)

    Publishing research using ab20962? Please let us know so that we can cite the reference in this datasheet.

    ab20962 has been referenced in 4 publications.

    • Ordonez AA  et al. Radiosynthesis and PET Bioimaging of 76Br-Bedaquiline in a Murine Model of Tuberculosis. ACS Infect Dis N/A:N/A (2019). PubMed: 31345032
    • Bouttier M  et al. Alu repeats as transcriptional regulatory platforms in macrophage responses to M. tuberculosis infection. Nucleic Acids Res N/A:N/A (2016). Human . PubMed: 27604870
    • Verway M  et al. Vitamin D induces interleukin-1ß expression: paracrine macrophage epithelial signaling controls M. tuberculosis infection. PLoS Pathog 9:e1003407 (2013). ICC/IF ; Mouse . PubMed: 23762029
    • Brzostek A  et al. Mycobacterium tuberculosis is able to accumulate and utilize cholesterol. J Bacteriol 191:6584-91 (2009). PubMed: 19717592

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-3 of 3 Abreviews or Q&A

    Question

    Our customer is looking for anti-BCG antibody for ICC/IF.

    Her sample is BCG infected human tumor cell line.

    I found that you recommended ab20962 as BCG antibody in scientific support Q2 of ab905.

    https://www.abcam.com/Mycobacterium-tuberculosis-antibody-ab905-support.html

    So I would like to know if it is possible to use mycobacterium tuberculosis antibody instead of BCG antibody.

    Please let me know.

    Best regards

    Read More

    Abcam community

    Verified customer

    Asked on May 30 2012

    Answer

    Thank you for your enquiry.

    I am sorry to confirm that after further investigation with our source, I can confirm that ab20962 has not specifically been tested for detection of BCG.

    If they would like totryit for detection of BCG, I would be pleased to providea testing discount. Please let me know if theyare interested.

    If you have any further questions, please do not hesitate to contact us.

    Read More

    Abcam Scientific Support

    Answered on May 30 2012

    Question

    Greetings,   I´m a PhD student in Health Sciences at the University of Minho (Portugal) and I need a biotin conjugated monoclonal antibody for Mycobacterium bovis BCG. I would like to know if you have this antibody and, if so, what product would you recommend me for my research. Could you also inform me about the price and availability of this antibody? You used to have a BCG antibody with the code ab34957, but I can no longer find it. Have you got any option? Thank you for your time and consideration. Best regards,  

    Read More

    Abcam community

    Verified customer

    Asked on Oct 24 2011

    Answer

    Thank you for your enquiry. I regret to inform you that the originator of this antibody no longer has sufficient stocks for us to be able to make this product available to our customers. We have therefore been forced to withdraw ab34957 from our product range. I do hope that this does not cause you too much inconvenience. I have conducted a search for you and found some alternative antibodies which may suit your need: - Unlabelled: ab43019 ab36731 ab905 ab20962 - Biotin-conjugated: ab20027 - it has only been tested in IHC-P I hope this helps and if I can assist further, please do not hesitate to contact me.

    Read More

    Abcam Scientific Support

    Answered on Oct 24 2011

    Question

    Our customer want to know more information about the immunogen of antibody ab20962. The immunogen is Purified PPD(purified protein derivative) He want to know is the immunogen a single protein from PPD or total protein from PPD.

    Read More

    Abcam community

    Verified customer

    Asked on Sep 06 2007

    Answer

    Thank you for your enquiry. I can confirm that PPD (purified protein derivative) immunogen for this Mycobacterium tuberculosis antibody is a commercially available preparation used in skin testing for tuberculosis. PPD tuberculin is a precipitate of non-species-specific molecules obtained from filtrates of sterilized, concentrated cultures. PPD is a mixture of proteins that appear as a smear on SDS-PAGE with molecular weights ranging from 10-65kDa. I hope this information is helpful. Should you have any further questions, please do not hesitate to contact us again.

    Read More

    Abcam Scientific Support

    Answered on Sep 06 2007

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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