FITC Anti-Mycobacterium tuberculosis antibody (ab20962)
Key features and details
- FITC Rabbit polyclonal to Mycobacterium tuberculosis
- Suitable for: IHC-P, ICC/IF
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG
Overview
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Product name
FITC Anti-Mycobacterium tuberculosis antibody
See all Mycobacterium tuberculosis primary antibodies -
Description
FITC Rabbit polyclonal to Mycobacterium tuberculosis -
Host species
Rabbit -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Tested applications
Suitable for: IHC-P, ICC/IFmore details -
Species reactivity
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Immunogen
Full length native protein (purified) corresponding to Mycobacterium tuberculosis. Purified PPD.
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General notes
The antibody is covalently coupled with high purity Isomer I of fluorescein isothiocyanate. Care is taken to ensure complete removal of any free fluorescein from the final product.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituents: 0.0268% PBS, 1% BSA -
Concentration information loading... -
Purity
IgG fraction -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab20962 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/10 - 1/50.
|
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| ICC/IF | (1) |
Use at an assay dependent dilution. Acetone fixation is recommended.
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| Notes |
|---|
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IHC-P
1/10 - 1/50. |
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ICC/IF
Use at an assay dependent dilution. Acetone fixation is recommended. |
Target
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Relevance
Mycobacterium tuberculosis is the most common cause of tuberculosis. Primary infection begins with inhalation of 1 to 10 aerosolised bacilli. The pathogenicity of the organism is determined by its ability to escape host immune responses as well as eliciting delayed hypersensitivity. Alveolar macrophages engulf the invading cells but are unable to mount an effective defense. Several virulence factors are responsible for this apparent failure; most notably in the mycobacterial cell wall are the cord factor, lipoarabinomannan, and the 65 kd heat shock protein or HSP65. The emergence of new strains of resistant Mycobacterium tuberculosis has created new interest in clinical diagnosis. Studies have shown immunohistochemical techniques to be superior to conventional special stains. Thus the demonstration of mycobacterial antigens are not only useful in establishing mycobacterial aetiology, but can also be used as an alternative method to the conventional Ziehl-Neelsen method. -
Alternative names
- M. tuberculosis antibody
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab20962 has been referenced in 5 publications.
- Genestet C et al. Rifampicin exposure reveals within-host Mycobacterium tuberculosis diversity in patients with delayed culture conversion. PLoS Pathog 17:e1009643 (2021). PubMed: 34166469
- Ordonez AA et al. Radiosynthesis and PET Bioimaging of 76Br-Bedaquiline in a Murine Model of Tuberculosis. ACS Infect Dis N/A:N/A (2019). PubMed: 31345032
- Bouttier M et al. Alu repeats as transcriptional regulatory platforms in macrophage responses to M. tuberculosis infection. Nucleic Acids Res N/A:N/A (2016). Human . PubMed: 27604870
- Verway M et al. Vitamin D induces interleukin-1ß expression: paracrine macrophage epithelial signaling controls M. tuberculosis infection. PLoS Pathog 9:e1003407 (2013). ICC/IF ; Mouse . PubMed: 23762029
- Brzostek A et al. Mycobacterium tuberculosis is able to accumulate and utilize cholesterol. J Bacteriol 191:6584-91 (2009). PubMed: 19717592