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  1. Link

    fitc-mycobacterium-tuberculosis-antibody-ab20962.pdf

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Microbiology Organism Bacteria Gram positive bacteria Mycobacterium
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FITC Anti-Mycobacterium tuberculosis antibody (ab20962)

  • Datasheet
  • SDS
Reviews (1)Q&A (3)References (5)

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Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Key features and details

  • FITC Rabbit polyclonal to Mycobacterium tuberculosis
  • Suitable for: IHC-P, ICC/IF
  • Conjugation: FITC. Ex: 493nm, Em: 528nm
  • Isotype: IgG

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Overview

  • Product name

    FITC Anti-Mycobacterium tuberculosis antibody
    See all Mycobacterium tuberculosis primary antibodies
  • Description

    FITC Rabbit polyclonal to Mycobacterium tuberculosis
  • Host species

    Rabbit
  • Conjugation

    FITC. Ex: 493nm, Em: 528nm
  • Tested applications

    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity

  • Immunogen

    Full length native protein (purified) corresponding to Mycobacterium tuberculosis. Purified PPD.

  • General notes

    The antibody is covalently coupled with high purity Isomer I of fluorescein isothiocyanate. Care is taken to ensure complete removal of any free fluorescein from the final product.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.1% Sodium azide
    Constituents: 0.0268% PBS, 1% BSA
  • Concentration information loading...
  • Purity

    IgG fraction
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Microbiology
    • Organism
    • Bacteria
    • Gram positive bacteria
    • Mycobacterium

Associated products

  • Isotype control

    • FITC-Rabbit IgG - Isotype Control (ab37406)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab20962 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
1/10 - 1/50.
ICC/IF (1)
Use at an assay dependent dilution. Acetone fixation is recommended.
Notes
IHC-P
1/10 - 1/50.
ICC/IF
Use at an assay dependent dilution. Acetone fixation is recommended.

Target

  • Relevance

    Mycobacterium tuberculosis is the most common cause of tuberculosis. Primary infection begins with inhalation of 1 to 10 aerosolised bacilli. The pathogenicity of the organism is determined by its ability to escape host immune responses as well as eliciting delayed hypersensitivity. Alveolar macrophages engulf the invading cells but are unable to mount an effective defense. Several virulence factors are responsible for this apparent failure; most notably in the mycobacterial cell wall are the cord factor, lipoarabinomannan, and the 65 kd heat shock protein or HSP65. The emergence of new strains of resistant Mycobacterium tuberculosis has created new interest in clinical diagnosis. Studies have shown immunohistochemical techniques to be superior to conventional special stains. Thus the demonstration of mycobacterial antigens are not only useful in establishing mycobacterial aetiology, but can also be used as an alternative method to the conventional Ziehl-Neelsen method.
  • Alternative names

    • M. tuberculosis antibody

Protocols

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (5)

Publishing research using ab20962? Please let us know so that we can cite the reference in this datasheet.

ab20962 has been referenced in 5 publications.

  • Genestet C  et al. Rifampicin exposure reveals within-host Mycobacterium tuberculosis diversity in patients with delayed culture conversion. PLoS Pathog 17:e1009643 (2021). PubMed: 34166469
  • Ordonez AA  et al. Radiosynthesis and PET Bioimaging of 76Br-Bedaquiline in a Murine Model of Tuberculosis. ACS Infect Dis N/A:N/A (2019). PubMed: 31345032
  • Bouttier M  et al. Alu repeats as transcriptional regulatory platforms in macrophage responses to M. tuberculosis infection. Nucleic Acids Res N/A:N/A (2016). Human . PubMed: 27604870
  • Verway M  et al. Vitamin D induces interleukin-1ß expression: paracrine macrophage epithelial signaling controls M. tuberculosis infection. PLoS Pathog 9:e1003407 (2013). ICC/IF ; Mouse . PubMed: 23762029
  • Brzostek A  et al. Mycobacterium tuberculosis is able to accumulate and utilize cholesterol. J Bacteriol 191:6584-91 (2009). PubMed: 19717592

Customer reviews and Q&As

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Immunocytochemistry/ Immunofluorescence abreview for FITC Anti-Mycobacterium tuberculosis antibody

Below Average
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Bacteria Cell (Mycobacterium marinum)
Permeabilization
Yes - Saponin
Specification
Mycobacterium marinum
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative
Paraformaldehyde
Read More

Robin Van den Biggelaar

Verified customer

Submitted Nov 16 2021

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