FITC Anti-Myeloperoxidase antibody [2C7] (ab11729)

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Flow Cytometry - FITC Anti-Myeloperoxidase antibody [2C7] (ab11729)

    Key features and details

    • FITC Mouse monoclonal [2C7] to Myeloperoxidase
    • Suitable for: Flow Cyt
    • Reacts with: Human
    • Conjugation: FITC. Ex: 493nm, Em: 528nm
    • Isotype: IgG1

    Overview

    • Product name

      FITC Anti-Myeloperoxidase antibody [2C7]
      See all Myeloperoxidase primary antibodies

    • Description

      FITC Mouse monoclonal [2C7] to Myeloperoxidase

    • Host species

      Mouse

    • Conjugation

      FITC. Ex: 493nm, Em: 528nm

    • Specificity

      ab11729 recognises human myeloperoxidase.

    • Tested applications

      Suitable for: Flow Cytmore details

    • Species reactivity

      Reacts with: Human

    • Immunogen

      Human myeloperoxidase

    • General notes

      This product should be stored undiluted. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    Applications

    Our Abpromise guarantee covers the use of ab11729 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt 1/1 - 1/10.

    Use 10 µl of the suggested working dilution to label 106 cells in 100 µl. This antibody may be used for the flow cytometric detection of myeloid cells following membrane permeablisation.

    ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

    Target

    • Function

      Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity.

    • Involvement in disease

      Defects in MPO are the cause of myeloperoxidase deficiency (MPD) [MIM:254600]. MPD is an autosomal recessive defect that results in disseminated candidiasis.

    • Sequence similarities

      Belongs to the peroxidase family. XPO subfamily.

    • Cellular localization

      Lysosome.
    • Information by UniProt

    • Database links

      • Alternative names

        • 84 kDa myeloperoxidase antibody
        • 89 kDa myeloperoxidase antibody
        • EC 1.11.1.7 antibody
        • EC1.11.2.2 antibody
        • fj80f04 antibody
        • MPO antibody
        • mpx antibody
        • myeloid-specific peroxidase antibody
        • Myeloperoxidase antibody
        • Myeloperoxidase heavy chain antibody
        • Myeloperoxidase light chain antibody
        • PERM_HUMAN antibody
        • wu:fj80f04 antibody
        see all

      Images

      • Flow Cytometry - FITC Anti-Myeloperoxidase antibody [2C7] (ab11729)
        Flow Cytometry - FITC Anti-Myeloperoxidase antibody [2C7] (ab11729)

        Staining of human peripheral blood granulocytes with ab11729 following permeabilisation with Leucoperm 

      References (8)

      Publishing research using ab11729? Please let us know so that we can cite the reference in this datasheet.

      ab11729 has been referenced in 8 publications.

      • Schneck E  et al. Flow Cytometry-Based Quantification of Neutrophil Extracellular Traps Shows an Association with Hypercoagulation in Septic Shock and Hypocoagulation in Postsurgical Systemic Inflammation-A Proof-of-Concept Study. J Clin Med 9:N/A (2020). PubMed: 31936385
      • Schneck E  et al. Blood Levels of Free-Circulating Mitochondrial DNA in Septic Shock and Postsurgical Systemic Inflammation and Its Influence on Coagulation: A Secondary Analysis of a Prospective Observational Study. J Clin Med 9:N/A (2020). PubMed: 32629885
      • Xu K  et al. Adenosine from a biologic source regulates neutrophil extracellular traps (NETs). J Leukoc Biol 105:1225-1234 (2019). PubMed: 30907983
      • Nesmond S  et al. Characteristic Pattern of IL-17RA, IL-17RB, and IL-17RC in Monocytes/Macrophages and Mast Cells From Patients With Bullous Pemphigoid. Front Immunol 10:2107 (2019). PubMed: 31572359
      • Bystrzycka W  et al. Azithromycin and Chloramphenicol Diminish Neutrophil Extracellular Traps (NETs) Release. Int J Mol Sci 18:N/A (2017). PubMed: 29292737
      • Bystrzycka W  et al. The effect of clindamycin and amoxicillin on neutrophil extracellular trap (NET) release. Cent Eur J Immunol 41:1-5 (2016). PubMed: 27095915
      • Gavillet M  et al. Flow cytometric assay for direct quantification of neutrophil extracellular traps in blood samples. Am J Hematol 90:1155-8 (2015). PubMed: 26347989
      • Nyåkern M  et al. Frequent elevation of Akt kinase phosphorylation in blood marrow and peripheral blood mononuclear cells from high-risk myelodysplastic syndrome patients. Leukemia 20:230-8 (2006). PubMed: 16341040

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