FITC Anti-Myeloperoxidase antibody [2C7] (ab11729)
![Flow Cytometry (Intracellular) - FITC Anti-Myeloperoxidase antibody [2C7] (ab11729)](https://www.abcam.com/ps/products/11/ab11729/Images/ab11729-273583-anti-myeloperoxidase-antibody-2c7-fitc-flow-cytometry.gif "Flow Cytometry (Intracellular) - FITC Anti-Myeloperoxidase antibody [2C7] (ab11729)")
Key features and details
- FITC Mouse monoclonal [2C7] to Myeloperoxidase
- Suitable for: Flow Cyt (Intra)
- Reacts with: Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG1
Related conjugates and formulations
Overview
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Product name
FITC Anti-Myeloperoxidase antibody [2C7]
See all Myeloperoxidase primary antibodies -
Description
FITC Mouse monoclonal [2C7] to Myeloperoxidase -
Host species
Mouse -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Tested applications
Suitable for: Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human Myeloperoxidase.
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Positive control
- human peripheral blood granulocytes: Flow Cyt (Intra).
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General notes
This product should be stored undiluted. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.40
Preservative: 0.09% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified IgG prepared by affinity chromatography. -
Clonality
Monoclonal -
Clone number
2C7 -
Myeloma
x63-Ag8.653 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Assay kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab11729 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/1 - 1/10.
Use 10µl of the suggested working dilution to label 106 cells in 100µl. This antibody may be used for the flow cytometric detection of myeloid cells following membrane permeablisation. ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
|
Flow Cyt (Intra)
1/1 - 1/10. Use 10µl of the suggested working dilution to label 106 cells in 100µl. This antibody may be used for the flow cytometric detection of myeloid cells following membrane permeablisation. ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
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Function
Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity. -
Involvement in disease
Defects in MPO are the cause of myeloperoxidase deficiency (MPD) [MIM:254600]. MPD is an autosomal recessive defect that results in disseminated candidiasis. -
Sequence similarities
Belongs to the peroxidase family. XPO subfamily. -
Cellular localization
Lysosome. - Information by UniProt
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Database links
- Entrez Gene: 4353 Human
- Omim: 606989 Human
- SwissProt: P05164 Human
- Unigene: 458272 Human
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Alternative names
- 84 kDa myeloperoxidase antibody
- 89 kDa myeloperoxidase antibody
- EC 1.11.1.7 antibody
see all
Images
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Flow Cytometry (Intracellular) - FITC Anti-Myeloperoxidase antibody [2C7] (ab11729)
Staining of human peripheral blood granulocytes with ab11729 following permeabilisation with Leucoperm
Protocols
Datasheets and documents
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Datasheet download
References (12)
ab11729 has been referenced in 12 publications.
- Schneck E et al. Flow Cytometry-Based Quantification of Neutrophil Extracellular Traps Shows an Association with Hypercoagulation in Septic Shock and Hypocoagulation in Postsurgical Systemic Inflammation-A Proof-of-Concept Study. J Clin Med 9:N/A (2020). PubMed: 31936385
- Schneck E et al. Blood Levels of Free-Circulating Mitochondrial DNA in Septic Shock and Postsurgical Systemic Inflammation and Its Influence on Coagulation: A Secondary Analysis of a Prospective Observational Study. J Clin Med 9:N/A (2020). PubMed: 32629885
- Manda-Handzlik A et al. Nitric oxide and peroxynitrite trigger and enhance release of neutrophil extracellular traps. Cell Mol Life Sci 77:3059-3075 (2020). PubMed: 31650185
- Mroczek A et al. Overexpression of ATG5 Gene Makes Granulocyte-Like HL-60 Susceptible to Release Reactive Oxygen Species. Int J Mol Sci 21:N/A (2020). PubMed: 32707918
- Kuzmicka W et al. Zinc Supplementation Modulates NETs Release and Neutrophils' Degranulation. Nutrients 13:N/A (2020). PubMed: 33375275
