Recombinant FITC Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab223994)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- FITC Rabbit monoclonal [EPR12763] to NeuN - Neuronal Marker
- Suitable for: ICC/IF, Flow Cyt
- Reacts with: Rat
- Conjugation: FITC. Ex: 493nm, Em: 528nm
Overview
-
Product name
FITC Anti-NeuN antibody [EPR12763] - Neuronal Marker
See all NeuN primary antibodies -
Description
FITC Rabbit monoclonal [EPR12763] to NeuN - Neuronal Marker -
Host species
Rabbit -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Tested applications
Suitable for: ICC/IF, Flow Cytmore details -
Species reactivity
Reacts with: Rat
Predicted to work with: Mouse, Human -
Immunogen
Synthetic peptide within Human NeuN aa 1-100 (Cysteine residue). The exact sequence is proprietary.
Database link: A6NFN3 -
Positive control
- Flow Cyt: PC12 cells. ICC/IF: PC12 cells
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol, 1% BSA -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR12763 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
- Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)
- Alexa Fluor® 488 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab190195)
- Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab190565)
- Biotin Anti-NeuN antibody [EPR12763] (ab204681)
- Alexa Fluor® 594 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab207279)
- Alexa Fluor® 555 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab207281)
- Alexa Fluor® 568 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab207282)
- Anti-NeuN antibody [EPR12763] - BSA and Azide free (ab209898)
-
Isotype control
Applications
Our Abpromise guarantee covers the use of ab223994 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF | 1/100. This product gave a positive signal in PC12 cells fixed with 100% methanol (5 min) |
|
Flow Cyt | 1/50. |
Target
-
Function
RNA-binding protein that regulates alternative splicing events. -
Sequence similarities
Contains 1 RRM (RNA recognition motif) domain. -
Cellular localization
Nucleus. Cytoplasm. - Information by UniProt
-
Database links
- Entrez Gene: 146713 Human
- Entrez Gene: 52897 Mouse
- Entrez Gene: 287847 Rat
- SwissProt: A6NFN3 Human
- SwissProt: Q8BIF2 Mouse
- Unigene: 135229 Human
- Unigene: 341103 Mouse
- Unigene: 143966 Rat
-
Alternative names
- FLJ56884 antibody
- FLJ58356 antibody
- Fox-1 homolog C antibody
see all
Images
-
Immunocytochemistry/ Immunofluorescence - FITC Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab223994)
with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab223994 at 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
-
Overlay histogram showing PC12 cells stained with ab223994 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab223994, 1/50 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was Rabbit IgG (monoclonal) FITC (ab223339) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (0)
ab223994 has not yet been referenced specifically in any publications.