RecombinantRabMAb

Recombinant FITC Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab223994)

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Immunocytochemistry/ Immunofluorescence - FITC Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab223994)
  • Flow Cytometry - FITC Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab223994)
  • FITC Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab223994)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • FITC Rabbit monoclonal [EPR12763] to NeuN - Neuronal Marker
  • Suitable for: ICC/IF, Flow Cyt
  • Reacts with: Rat
  • Conjugation: FITC. Ex: 493nm, Em: 528nm

Overview

  • Product name

    FITC Anti-NeuN antibody [EPR12763] - Neuronal Marker
    See all NeuN primary antibodies

  • Description

    FITC Rabbit monoclonal [EPR12763] to NeuN - Neuronal Marker

  • Host species

    Rabbit

  • Conjugation

    FITC. Ex: 493nm, Em: 528nm

  • Tested applications

    Suitable for: ICC/IF, Flow Cytmore details

  • Species reactivity

    Reacts with: Rat
    Predicted to work with: Mouse, Human

  • Immunogen

    Synthetic peptide within Human NeuN aa 1-100 (Cysteine residue). The exact sequence is proprietary.
    Database link: A6NFN3

  • Positive control

    • Flow Cyt: PC12 cells. ICC/IF: PC12 cells

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab223994 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.

This product gave a positive signal in PC12 cells fixed with 100% methanol (5 min)
Flow Cyt 1/50.

Target

Images

  • Immunocytochemistry/ Immunofluorescence - FITC Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab223994)
    Immunocytochemistry/ Immunofluorescence - FITC Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab223994)

    with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab223994 at 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Flow Cytometry - FITC Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab223994)
    Flow Cytometry - FITC Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab223994)

    Overlay histogram showing PC12 cells stained with ab223994 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab223994, 1/50 dilution) for 30 min at 22°C.

    Isotype control antibody (black line) was Rabbit IgG (monoclonal) FITC (ab223339) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter.

  • FITC Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab223994)
    FITC Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab223994)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab223994? Please let us know so that we can cite the reference in this datasheet.

ab223994 has not yet been referenced specifically in any publications.

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