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  1. Link

    fitc-neun-antibody-epr12763-neuronal-marker-ab223994.pdf

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Neuroscience Cell Type Marker Neuron marker Soma marker
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RecombinantRabMAb

Recombinant FITC Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab223994)

  • Datasheet
  • SDS
  • Certificate of Compliance
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Immunocytochemistry/ Immunofluorescence - FITC Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab223994)
  • Immunocytochemistry/ Immunofluorescence - FITC Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab223994)
  • Flow Cytometry (Intracellular) - FITC Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab223994)
  • FITC Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab223994)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • FITC Rabbit monoclonal [EPR12763] to NeuN - Neuronal Marker
  • Suitable for: ICC/IF, Flow Cyt (Intra)
  • Reacts with: Rat, Human
  • Conjugation: FITC. Ex: 493nm, Em: 528nm

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Biotin Carrier Free PE Unconjugated

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Anti-NeuN antibody [EPR12763] - BSA and Azide free (ab209898)

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Overview

  • Product name

    FITC Anti-NeuN antibody [EPR12763] - Neuronal Marker
    See all NeuN primary antibodies
  • Description

    FITC Rabbit monoclonal [EPR12763] to NeuN - Neuronal Marker
  • Host species

    Rabbit
  • Conjugation

    FITC. Ex: 493nm, Em: 528nm
  • Tested applications

    Suitable for: ICC/IF, Flow Cyt (Intra)more details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Flow Cyt (intra): PC12 cells. ICC/IF: PC12 and B35 cells cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR12763
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Soma marker
    • Tags & Cell Markers
    • Cell Type Markers
    • Neuroscience Markers
    • Neuronal
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Forkhead Box
    • Other FOXes
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors

Associated products

  • Alternative Versions

    • Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)
    • Alexa Fluor® 488 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab190195)
    • Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab190565)
    • Biotin Anti-NeuN antibody [EPR12763] (ab204681)
    • Alexa Fluor® 594 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab207279)
    • Alexa Fluor® 555 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab207281)
    • Alexa Fluor® 568 Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab207282)
    • Anti-NeuN antibody [EPR12763] - BSA and Azide free (ab209898)
  • Isotype control

    • FITC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab223339)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab223994 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
1/100.

This product gave a positive signal in PC12 cells fixed with 100% methanol (5 min)

Flow Cyt (Intra)
1/50.
Notes
ICC/IF
1/100.

This product gave a positive signal in PC12 cells fixed with 100% methanol (5 min)

Flow Cyt (Intra)
1/50.

Target

  • Function

    RNA-binding protein that regulates alternative splicing events.
  • Sequence similarities

    Contains 1 RRM (RNA recognition motif) domain.
  • Cellular localization

    Nucleus. Cytoplasm.
  • Target information above from: UniProt accession A6NFN3 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 146713 Human
    • Entrez Gene: 52897 Mouse
    • Entrez Gene: 287847 Rat
    • SwissProt: A6NFN3 Human
    • SwissProt: Q8BIF2 Mouse
    • Unigene: 135229 Human
    • Unigene: 341103 Mouse
    • Alternative names

      • FLJ56884 antibody
      • FLJ58356 antibody
      • Fox-1 homolog C antibody
      • fox1 homolog C antibody
      • Fox3 antibody
      • FOX3NeuN antibody
      • hexaribonucleotide binding protein 3 antibody
      • HRNBP3 antibody
      • NEUN antibody
      • neuronal nuclei antibody
      • Rbfox3 antibody
      • RFOX3_HUMAN antibody
      • RNA binding protein fox-1 homolog 3 antibody
      • RNA binding protein, fox 1 homolog (C. elegans) 3 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - FITC Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab223994)
      Immunocytochemistry/ Immunofluorescence - FITC Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab223994)

      ab223994 staining NeuN in B35 cells. The cells were fixed with 4% PFA (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab223994 at 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    • Immunocytochemistry/ Immunofluorescence - FITC Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab223994)
      Immunocytochemistry/ Immunofluorescence - FITC Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab223994)

      ab223994 staining NeuN in PC12 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab223994 at 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    • Flow Cytometry (Intracellular) - FITC Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab223994)
      Flow Cytometry (Intracellular) - FITC Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab223994)

      Overlay histogram showing PC12 cells stained with ab223994 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab223994, 1/50 dilution) for 30 min at 22°C.

      Isotype control antibody (black line) was Rabbit IgG (monoclonal) FITC (ab223339) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

      Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter.

    • FITC Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab223994)
      FITC Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab223994)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (1)

    Publishing research using ab223994? Please let us know so that we can cite the reference in this datasheet.

    ab223994 has been referenced in 1 publication.

    • Michael BD  et al. Astrocyte- and Neuron-Derived CXCL1 Drives Neutrophil Transmigration and Blood-Brain Barrier Permeability in Viral Encephalitis. Cell Rep 32:108150 (2020). PubMed: 32937134

    Customer reviews and Q&As

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