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  1. Link

    fitc-neutrophil-antibody-74-ab53453.pdf

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FITC Anti-Neutrophil antibody [7/4] (ab53453)

  • Datasheet
Reviews (5)Q&A (3)References (19)

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Abpromise

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Flow Cytometry - FITC Anti-Neutrophil antibody [7/4] (ab53453)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FITC Anti-Neutrophil antibody [7/4] (ab53453)

Key features and details

  • FITC Rat monoclonal [7/4] to Neutrophil
  • Suitable for: Flow Cyt, IHC-P
  • Reacts with: Mouse
  • Conjugation: FITC. Ex: 493nm, Em: 528nm
  • Isotype: IgG2a

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Conjugation
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FITC Conjugation Kit - Lightning-Link® (ab102884)

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Overview

  • Product name

    FITC Anti-Neutrophil antibody [7/4]
    See all Neutrophil primary antibodies
  • Description

    FITC Rat monoclonal [7/4] to Neutrophil
  • Host species

    Rat
  • Conjugation

    FITC. Ex: 493nm, Em: 528nm
  • Specificity

    Rat anti mouse Ly-6B.2, clone 7/4 recognizes the Ly-6B.2 antigen in 129J; AKR; C57BL/6; C57BL/10; C58; DBA/2; NZB; NZW; SJL; MFI; Swiss (PO) strains. A2G; A/Sn; ASW; BALB/c; C3H/HEH: CBA.T6T6 are negative or demonstrate very weak reactivity.
  • Tested applications

    Suitable for: Flow Cyt, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Tissue/ cell preparation: cultured bone marrow cells.

  • Epitope

    Recognizes the Ly-6B.2 antigen.
  • Positive control

    • Flow Cyt.: New Zealand Black mouse peripheral blood granulocytes.
  • General notes

    ab53453 recognizes the Ly-6B.2 antigen. Ly-6B.2 is a ~25-30 kDa GPI-anchored, heavily glycosylated protein expressed on neutrophils, inflammatory monocytes and some activated macrophages. High levels of expression are seen in bone marrow, spleen, lung and lymph nodes. N-glycanase treatment of thioglycollate elicited peritoneal neutrophil lysates lowers the apparent molecular weight of Ly-6B.2 to ~15 kDa.

    ab53453 has been successfully used for the immunomagnetic depletion of neutrophils during the enrichment of primitive hematopoietic cells from bone marrow and the depletion of myeloid cells in vivo.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 1% BSA, PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
  • Primary antibody notes

    ab53453 recognises a neutrophil allotypic marker, a polymorphic 40kD antigen expressed by polymorphonuclear cells, but absent on resident tissue macrophages.
  • Clonality

    Monoclonal
  • Clone number

    7/4
  • Myeloma

    Y3/Ag1.2.3
  • Isotype

    IgG2a
  • Research areas

    • Cardiovascular
    • Blood
    • Other

Associated products

  • Alternative Versions

    • PE Anti-Neutrophil antibody [7/4] (ab105157)
    • Anti-Neutrophil antibody [7/4] (ab53457)
  • Isotype control

    • FITC Rat IgG2a, kappa monoclonal [RTK2758] - Isotype control (ab18446)

Applications

Our Abpromise guarantee covers the use of ab53453 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

Use 10µl of the working dilution to label 106 cells in 100µl.
ab18446 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

IHC-P Use at an assay dependent concentration.

Target

  • Cellular localization

    Cell membrane; Lipid-anchor, GPI-anchor
  • Database links

    • Entrez Gene: 110453 Mouse
    • Alternative names

      • Ly-6B antibody
      • Ly6b antibody
      • Lymphocyte antigen 6B antibody
      • Lymphocyte antigen 6B Short name=Ly-6B antibody

    Images

    • Flow Cytometry - FITC Anti-Neutrophil antibody [7/4] (ab53453)
      Flow Cytometry - FITC Anti-Neutrophil antibody [7/4] (ab53453)
      Staining of New Zealand Black mouse peripheral blood granulocytes with Rat anti mouse neutrophil:FITC (ab53453).
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FITC Anti-Neutrophil antibody [7/4] (ab53453)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FITC Anti-Neutrophil antibody [7/4] (ab53453)

      Immunohistochemical analysis of mouse spleen cryosection tissue stained for Neutrophil using ab53453.

    Protocols

    • Flow cytometry protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (19)

    Publishing research using ab53453? Please let us know so that we can cite the reference in this datasheet.

    ab53453 has been referenced in 19 publications.

    • Kang L  et al. Neutrophil extracellular traps released by neutrophils impair revascularization and vascular remodeling after stroke. Nat Commun 11:2488 (2020). PubMed: 32427863
    • Hensel JA  et al. Characterization of immune cell subtypes in three commonly used mouse strains reveals gender and strain-specific variations. Lab Invest 99:93-106 (2019). PubMed: 30353130
    • Esposito E  et al. Brain-to-cervical lymph node signaling after stroke. Nat Commun 10:5306 (2019). PubMed: 31757960
    • Csepregi JZ  et al. Myeloid-Specific Deletion of Mcl-1 Yields Severely Neutropenic Mice That Survive and Breed in Homozygous Form. J Immunol 201:3793-3803 (2018). PubMed: 30464050
    • Halim TYF  et al. Tissue-Restricted Adaptive Type 2 Immunity Is Orchestrated by Expression of the Costimulatory Molecule OX40L on Group 2 Innate Lymphoid Cells. Immunity 48:1195-1207.e6 (2018). PubMed: 29907525
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-8 of 8 Abreviews or Q&A

    Question

    What is the meaning of Swiss (PO) in the listed species on the datasheet?

    Read More

    Abcam community

    Verified customer

    Asked on Aug 27 2013

    Answer

    Swiss PO is used to denote "Swiss-Pathology Oxford," a specific strain at Oxford University.

    Read More

    Brendan Collins

    Abcam Scientific Support

    Answered on Aug 27 2013

    Flow Cytometry abreview for Anti-Neutrophil antibody [7/4] (FITC)

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Dog Cell (from whole blood)
    Specification
    from whole blood
    Preparation
    Cell harvesting/tissue preparation method: after red blood cell lysis
    Sample buffer: PBS/BSA 1%
    Fixation
    Paraformaldehyde
    Permeabilization
    No
    Gating Strategy
    alive neutrophils
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 12 2012

    Flow Cytometry abreview for Anti-Neutrophil antibody [7/4] (FITC)

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Cynomolgus Monkey Cell (Whole blood)
    Specification
    Whole blood
    Preparation
    Cell harvesting/tissue preparation method: After red blood cell lysis
    Sample buffer: PBS/BSA 1%/Azide 0.01%
    Fixation
    Paraformaldehyde
    Permeabilization
    No
    Gating Strategy
    alive granulocytes
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 27 2012

    Flow Cytometry abreview for Anti-Neutrophil antibody [7/4] (FITC)

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Rat Cell (Splenocytes)
    Specification
    Splenocytes
    Preparation
    Cell harvesting/tissue preparation method: spleen was mashed and red blood cells lysed
    Sample buffer: PBS/BSA 1%/Azide 0.01%
    Permeabilization
    No
    Gating Strategy
    alive monocytes
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 27 2012

    Immunohistochemistry (Frozen sections) abreview for Anti-Neutrophil antibody [7/4] (FITC)

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Spleen from immune mouse)
    Permeabilization
    No
    Specification
    Spleen from immune mouse
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Acetone
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 18 2012

    Flow Cytometry abreview for Anti-Neutrophil antibody [7/4] (FITC)

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Mouse Cell (cells from blood)
    Specification
    cells from blood
    Preparation
    Cell harvesting/tissue preparation method: after red blood cell lysis
    Sample buffer: PBS/BSA 1%
    Permeabilization
    No
    Gating Strategy
    neutrophils
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 20 2011

    Question

    Hi I wonder about the quantity of antibody in your ´anti-neutrophil antibody 7/4` product. (ab 105157). Is it comparable to the FITC-conjugated antibody? Best regards Linda

    Read More

    Abcam community

    Verified customer

    Asked on Nov 18 2011

    Answer

    Thank you for your enquiry. I am pleased to confirm the following information: ab105157 Datasheet suggests 10 ul per test 50 tests = 500 ul ab53453 On the datasheet, provide 50 ug at 0.1 mg/ml = 500ul I hope this will be helpful to you. If you have any further questions, please do not hesitate to contact us.

    Read More

    Abcam Scientific Support

    Answered on Nov 18 2011

    Question

    Dear Sir/ Madam, I am PhD student in Sydney University. I have Rat and Mice paraffin and frozen skin tissue. I want to measure Neutrophils. According to your experience would you please lead me whether ab45977 is suitable primary antibody or not. Thank you for your cooperation. Best Regards,

    Read More

    Abcam community

    Verified customer

    Asked on Sep 19 2011

    Answer

    Thank you for contacting us. Please check the following catalogue number; ab53457 ab53453 ab105157 ab2557 These are exact match to your requirements however I would recommend reading the datasheets carefully and buying the one as per your requirements. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on Sep 19 2011

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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