FITC Anti-Nitrotyrosine antibody [39B6] (ab183390)
Key features and details
- FITC Mouse monoclonal [39B6] to Nitrotyrosine
- Suitable for: WB, IHC-Fr, ICC/IF, IP, IHC-P
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG2a
Overview
-
Product name
FITC Anti-Nitrotyrosine antibody [39B6]
See all Nitrotyrosine primary antibodies -
Description
FITC Mouse monoclonal [39B6] to Nitrotyrosine -
Host species
Mouse -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Specificity
ab183390 recognizes 3-nitrotyrosine moieties. No detectable cross-reactivity with non-nitrated tyrosine. Not species specific. -
Tested applications
Suitable for: WB, IHC-Fr, ICC/IF, IP, IHC-Pmore details -
Immunogen
Chemical/ Small Molecule corresponding to Nitrotyrosine conjugated to Bovine Serum Albumin (BSA). 3-(4-Hydroxy-3-nitrophenyl acetamido) propionic acid-BSA conjugate.
-
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Store In the Dark. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
39B6 -
Isotype
IgG2a -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Related Products
Applications
Our Abpromise guarantee covers the use of ab183390 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 0.7 µg/ml. 0.7μg/ml of ab183390 was sufficient for detection of 5μg SIN-1 treated BSA by Western Blot analysis using Goat anti-mouse IgG (HRP) as the secondary antibody. |
|
| IHC-Fr | Use at an assay dependent concentration. | |
| ICC/IF | Use at an assay dependent concentration. | |
| IP | Use at an assay dependent concentration. | |
| IHC-P | Use at an assay dependent concentration. |
Target
-
Relevance
The cellular production of highly reactive nitrogen species derived from nitric oxide, such as peroxynitrite, nitrogen dioxide and nitryl chloride, leads to the nitration of tyrosine resides in tissue proteins. The extent of protein nitrotyrosine formation provides an index of the production of reactive nitrogen species and potential cell damage over a period of time. Nitrotyrosine can be measured by amino-acid analysis of protein hydrolysates and detected, estimated semi-quantitatively and located in cells and tissues by immunocytochemical techniques using antibodies directed against the nitrotyrosine hapten.
Protocols
Datasheets and documents
References (0)
ab183390 has not yet been referenced specifically in any publications.
