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  1. Link

    fitc-nkr-p1c-antibody-pk136-ab25026.pdf

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Immunology Cell Type Markers CD NK Cells
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FITC Anti-NKR-P1C antibody [PK136] (ab25026)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (5)

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Key features and details

  • FITC Mouse monoclonal [PK136] to NKR-P1C
  • Reacts with: Mouse
  • Conjugation: FITC. Ex: 493nm, Em: 528nm
  • Isotype: IgG2a

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Conjugation
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FITC Conjugation Kit - Lightning-Link® (ab102884)

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Overview

  • Product name

    FITC Anti-NKR-P1C antibody [PK136]
    See all NKR-P1C primary antibodies
  • Description

    FITC Mouse monoclonal [PK136] to NKR-P1C
  • Host species

    Mouse
  • Conjugation

    FITC. Ex: 493nm, Em: 528nm
  • Specificity

    This antibody is specific to Mouse NKR-P1C alloantigen, Mr 80-85 kDa (39 kDa under reducing conditions).
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Tissue, cells or virus corresponding to NKR-P1C. CE mouse spleen enriched for NK-1+ cells and bone marrow cells

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.09% Sodium azide
    Constituents: PBS, 0.87% Sodium chloride, 0.1% Gelatin, 0.14% Monobasic dihydrogen sodium phosphate
  • Concentration information loading...
  • Purity

    Affinity purified
  • Purification notes

    Purified from TCS
  • Clonality

    Monoclonal
  • Clone number

    PK136
  • Isotype

    IgG2a
  • Light chain type

    kappa
  • Research areas

    • Immunology
    • Cell Type Markers
    • CD
    • NK Cells
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • T Lymphocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • NK Cell Lineage

Associated products

  • Alternative Versions

    • Anti-NKR-P1C antibody [PK136] - BSA and Azide free (ab174600)
    • PerCP/Cy5.5® Anti-NKR-P1C antibody [PK136] (ab210337)
    • redFluor™ 710 Anti-NKR-P1C antibody [PK136] (ab242071)
    • PE Anti-NKR-P1C antibody [PK136] (ab25027)
  • Isotype control

    • FITC Mouse IgG2a, kappa monoclonal [MOPC-173] - isotype control (ab18455)

Target

  • Function

    Plays a stimulatory role on natural killer (NK) cells cytotoxicity. Activation by cross-linking of the receptor induces Ca(2+) mobilization and interferon-gamma production.
  • Tissue specificity

    Expressed in natural killer cells.
  • Sequence similarities

    Contains 1 C-type lectin domain.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession P27814 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 17059 Mouse
    • SwissProt: P27814 Mouse
    • Unigene: 480764 Mouse
    • Alternative names

      • CD161 antigen like family member C antibody
      • CD161 antigen-like family member C antibody
      • CD161c antibody
      • Killer cell lectin-like receptor subfamily B member 1C antibody
      • Klrb1c antibody
      • Klrb1f antibody
      • KLRBC_MOUSE antibody
      • Ly-55c antibody
      • Ly55c antibody
      • Lymphocyte antigen 55c antibody
      • Lymphocyte antigen 59 antibody
      • Natural killer cell surface protein P1-40 antibody
      • NK1.1 antibody
      • NKR P1.9 antibody
      • NKR P1C antibody
      • NKR-P1 40 antibody
      • NKR-P1.9 antibody
      • NKR-P1C antibody
      • NKRP1 antibody
      see all

    Protocols

    • Flow cytometry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (5)

    Publishing research using ab25026? Please let us know so that we can cite the reference in this datasheet.

    ab25026 has been referenced in 5 publications.

    • McAllister CS  et al. TLR3, TRIF, and caspase 8 determine double-stranded RNA-induced epithelial cell death and survival in vivo. J Immunol 190:418-27 (2013). ICC/IF . PubMed: 23209324
    • Karlhofer FM & Yokoyama WM Stimulation of murine natural killer (NK) cells by a monoclonal antibody specific for the NK1.1 antigen. IL-2-activated NK cells possess additional specific stimulation pathways. J Immunol 146:3662-73 (1991). PubMed: 2026886
    • Sentman CL  et al. Pan natural killer cell monoclonal antibodies and their relationship to the NK1.1 antigen. Hybridoma 8:605-14 (1989). PubMed: 2613267
    • Koo GC  et al. The NK-1.1(-) mouse: a model to study differentiation of murine NK cells. J Immunol 137:3742-7 (1986). PubMed: 3782794
    • Koo GC & Peppard JR Establishment of monoclonal anti-Nk-1.1 antibody. Hybridoma 3:301-3 (1984). PubMed: 6500587

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-NKR-P1C antibody [PK136] (FITC)

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (spleen)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10 mM Citrate buffer, pH 6.0
    Permeabilization
    No
    Specification
    spleen
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 30% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted May 21 2019

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