FITC Anti-OX40L/TNFSF4 antibody [ATM-2] (ab124047)

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Flow Cytometry - FITC Anti-OX40L/TNFSF4 antibody [ATM-2] (ab124047)

    Key features and details

    • FITC Mouse monoclonal [ATM-2] to OX40L/TNFSF4
    • Suitable for: Flow Cyt
    • Reacts with: Rat
    • Conjugation: FITC. Ex: 493nm, Em: 528nm
    • Isotype: IgG1

    Overview

    • Product name

      FITC Anti-OX40L/TNFSF4 antibody [ATM-2]
      See all OX40L/TNFSF4 primary antibodies

    • Description

      FITC Mouse monoclonal [ATM-2] to OX40L/TNFSF4

    • Host species

      Mouse

    • Conjugation

      FITC. Ex: 493nm, Em: 528nm

    • Tested applications

      Suitable for: Flow Cytmore details

    • Species reactivity

      Reacts with: Rat

    • Immunogen

      ACI-S1 Rat T cell lymphoma cell line.

    • Positive control

      • LPS stimulated Rat splenocytes

    • General notes

      ab124047 should be stored undiluted, and in the dark.


      Clone ATM-2 has been shown to block the costimulatory activity of CD252 on Rat T cell proliferation.

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    Applications

    Our Abpromise guarantee covers the use of ab124047 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt 1/1.

    Use 10 µl of the suggested working dilution to label 1x106 cells in 100 µl.

     

     

     

    ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

     

    Target

    • Function

      Cytokine that binds to TNFRSF4. Co-stimulates T-cell proliferation and cytokine production.

    • Involvement in disease

      Genetic variations in TNFSF4 influence susceptibility to systemic lupus erythematosus (SLE) [MIM:152700]. SLE is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system. Note=The upstream region of TNFSF4 contains a single risk haplotype for SLE, which is correlated with increased expression of both cell-surface TNFSF4 and TNFSF4 transcripts. Increased levels of TNFSF4 are thought to augment T cell-APC interaction and the functional consequences of T cell activation, thereby destabilizing peripheral tolerance.

    • Sequence similarities

      Belongs to the tumor necrosis factor family.

    • Cellular localization

      Membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • CD 134L antibody
        • CD 252 antibody
        • CD134 ligand antibody
        • CD134L antibody
        • CD252 antibody
        • CD252 antigen antibody
        • glycoprotein 34 kd antibody
        • Glycoprotein Gp34 antibody
        • GP34 antibody
        • OX-40L antibody
        • OX40 antigen ligand antibody
        • OX40 ligand antibody
        • OX40L antibody
        • TAX transcriptionally-activated glycoprotein 1 antibody
        • TNFL4_HUMAN antibody
        • Tnfsf4 antibody
        • Tumor necrosis factor (ligand) superfamily member 4 antibody
        • Tumor necrosis factor ligand superfamily member 4 antibody
        • TXGP1 antibody
        see all

      Images

      • Flow Cytometry - FITC Anti-OX40L/TNFSF4 antibody [ATM-2] (ab124047)
        Flow Cytometry - FITC Anti-OX40L/TNFSF4 antibody [ATM-2] (ab124047)

        ab124032, at 10 µl per 1x106 cells in 100 µl, staining OX40L/TNFSF4 in LPS stimulated Rat splenocytes by Flow Cytometry.

      References (2)

      Publishing research using ab124047? Please let us know so that we can cite the reference in this datasheet.

      ab124047 has been referenced in 2 publications.

      • Yuan X  et al. The role of the CD134-CD134 ligand costimulatory pathway in alloimmune responses in vivo. J Immunol 170:2949-55 (2003). PubMed: 12626546
      • Satake Y  et al. Characterization of rat OX40 ligand by monoclonal antibody. Biochem Biophys Res Commun 270:1041-8 (2000). PubMed: 10772947

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