Key features and details
- FITC Mouse monoclonal [ATM-2] to OX40L/TNFSF4
- Suitable for: Flow Cyt
- Reacts with: Rat
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG1
Product nameFITC Anti-OX40L/TNFSF4 antibody [ATM-2]
See all OX40L/TNFSF4 primary antibodies
DescriptionFITC Mouse monoclonal [ATM-2] to OX40L/TNFSF4
ConjugationFITC. Ex: 493nm, Em: 528nm
Tested applicationsSuitable for: Flow Cytmore details
Species reactivityReacts with: Rat
ACI-S1 Rat T cell lymphoma cell line.
- LPS stimulated Rat splenocytes
General notesab124047 should be stored undiluted, and in the dark.
Clone ATM-2 has been shown to block the costimulatory activity of CD252 on Rat T cell proliferation.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 98% PBS, 1% BSA
Concentration information loading...
PurityProtein G purified
Purification notesPurified IgG was prepared by affinity chromatography on Protein G from tissue culture supernatant.
Primary antibody notesClone ATM-2 has been shown to block the costimulatory activity of CD252 on Rat T cell proliferation.
Our Abpromise guarantee covers the use of ab124047 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 10 µl of the suggested working dilution to label 1x106 cells in 100 µl.
ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
FunctionCytokine that binds to TNFRSF4. Co-stimulates T-cell proliferation and cytokine production.
Involvement in diseaseGenetic variations in TNFSF4 influence susceptibility to systemic lupus erythematosus (SLE) [MIM:152700]. SLE is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system. Note=The upstream region of TNFSF4 contains a single risk haplotype for SLE, which is correlated with increased expression of both cell-surface TNFSF4 and TNFSF4 transcripts. Increased levels of TNFSF4 are thought to augment T cell-APC interaction and the functional consequences of T cell activation, thereby destabilizing peripheral tolerance.
Sequence similaritiesBelongs to the tumor necrosis factor family.
- Information by UniProt
- CD 134L antibody
- CD 252 antibody
- CD134 ligand antibody
ab124032, at 10 µl per 1x106 cells in 100 µl, staining OX40L/TNFSF4 in LPS stimulated Rat splenocytes by Flow Cytometry.
ab124047 has been referenced in 2 publications.