Key features and details
- FITC Mouse monoclonal [B-D48] to Perforin, prediluted
- Reacts with: Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG1
Product nameFITC Anti-Perforin antibody [B-D48], prediluted
See all Perforin primary antibodies
DescriptionFITC Mouse monoclonal [B-D48] to Perforin, prediluted
ConjugationFITC. Ex: 493nm, Em: 528nm
Species reactivityReacts with: Human
Natural Human Perforin
This product was changed from ascites to tissue culture supernatant on 19th June 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
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Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.3
Preservative: 0.1% Sodium azide
Constituents: 5% BSA, PBS
Concentration information loading...
PurityTissue culture supernatant
FunctionPlays a key role in secretory granule-dependent cell death, and in defense against virus-infected or neoplastic cells. Plays an important role in killing other cells that are recognized as non-self by the immune system, e.g. in transplant rejection or some forms of autoimmune disease. Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores. Promotes cytolysis and apoptosis of target cells by facilitating the uptake of cytotoxic granzymes.
Involvement in diseaseDefects in PRF1 are the cause of hemophagocytic lymphohistiocytosis familial type 2 (FHL2) [MIM:603553]; also known as HPLH2. Familial hemophagocytic lymphohistiocytosis (FHL) is a genetically heterogeneous, rare autosomal recessive disorder. It is characterized by immune dysregulation with hypercytokinemia and defective natural killer cell function. The clinical features of the disease include fever, hepatosplenomegaly, cytopenia, hypertriglyceridemia, hypofibrinogenemia, and neurological abnormalities ranging from irritability and hypotonia to seizures, cranial nerve deficits, and ataxia. Hemophagocytosis is a prominent feature of the disease, and a non-malignant infiltration of macrophages and activated T lymphocytes in lymph nodes, spleen, and other organs is also found.
Sequence similaritiesBelongs to the complement C6/C7/C8/C9 family.
Contains 1 C2 domain.
Contains 1 EGF-like domain.
Contains 1 MACPF domain.
DomainThe C2 domain mediates calcium-dependent binding to lipid membranes. A subsequent conformation change leads to membrane insertion of beta-hairpin structures and pore formation. The pore is formed by transmembrane beta-strands.
Cellular localizationCytoplasmic granule lumen. Secreted. Cell membrane. Endosome lumen. Stored in cytoplasmic granules of cytolytic T-lymphocytes and secreted into the cleft between T-lymphocyte and target cell. Inserts into the cell membrane of target cells and forms pores. Membrane insertion and pore formation requires a major conformation change. May be taken up via endocytosis involving clathrin-coated vesicles and accumulate in a first time in large early endosomes.
- Information by UniProt
- Cytolysin antibody
- FLH2 antibody
- HPLH2 antibody
ab86318 has been referenced in 2 publications.
- Lesteberg K et al. Recycling endosomes in human cytotoxic T lymphocytes constitute an auxiliary intracellular trafficking pathway for newly synthesized perforin. Immunol Res 65:1031-1045 (2017). Human . PubMed: 28822075
- De Keersmaecker B et al. Expansion of polyfunctional HIV-specific T cells upon stimulation with mRNA electroporated dendritic cells in the presence of immunomodulatory drugs. J Virol : (2012). Flow Cyt ; Human . PubMed: 22718823