Key features and details
- FITC Mouse monoclonal [18H5] to Podoplanin / gp36
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG1
Product nameFITC Anti-Podoplanin / gp36 antibody [18H5]
See all Podoplanin / gp36 primary antibodies
DescriptionFITC Mouse monoclonal [18H5] to Podoplanin / gp36
ConjugationFITC. Ex: 493nm, Em: 528nm
Tested applicationsSuitable for: Flow Cytmore details
Species reactivityReacts with: Human
Tissue, cells or virus corresponding to Human Podoplanin/ gp36. NP_001006625.1. MDCK cells stably expressing Podoplanin/gp36 protein isoform 6.
Database link: Q86YL7-6
- Primary human dermal lymphatic endothelial cells (HDLEC).
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We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze. Store In the Dark.
Storage bufferPreservative: 0.02% Sodium azide
Constituents: 98% PBS, 1% BSA
Concentration information loading...
PurityProtein G purified
Purification notespurified from cell culture supernatant
Our Abpromise guarantee covers the use of ab205333 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration.
The suggested use of the antibody is ≤ 0.5 μg in 100 μl volume. It is recommended that the conjugate antibody should be titrated for optimal performance for each application.
FunctionMay be involved in cell migration and/or actin cytoskeleton organization. When expressed in keratinocytes, induces changes in cell morphology with transfected cells showing an elongated shape, numerous membrane protrusions, major reorganization of the actin cytoskeleton, increased motility and decreased cell adhesion. Required for normal lung cell proliferation and alveolus formation at birth. Induces platelet aggregation. Does not have any effect on folic acid or amino acid transport. Does not function as a water channel or as a regulator of aquaporin-type water channels.
Tissue specificityHighly expressed in placenta, lung, skeletal muscle and brain. Weakly expressed in brain, kidney and liver. In placenta, expressed on the apical plasma membrane of endothelium. In lung, expressed in alveolar epithelium. Up-regulated in colorectal tumors and expressed in 25% of early oral squamous cell carcinomas.
Sequence similaritiesBelongs to the podoplanin family.
modificationsExtensively O-glycosylated. Contains sialic acid residues. O-glycosylation is necessary for platelet aggregation activity.
The N-terminus is blocked.
Cellular localizationMembrane. Cell projection > filopodium membrane. Cell projection > lamellipodium membrane. Cell projection > microvillus membrane. Cell projection > ruffle membrane. Localized to actin-rich microvilli and plasma membrane projections such as filopodia, lamellipodia and ruffles.
- Information by UniProt
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Flow cytometric analysis of primary Human dermal lymphatic endothelial cells (HDLEC) labeling Podoplanin / gp36 with ab205333 at 0.5 µg in 100 µl dilution (lower panel) compared to negative control cells containing no primary antibody (upper panel) or conjugated secondary antibody alone (middle panel).
Flow cytometric analysis of primary Human dermal lymphatic endothelial cells (HDLEC) labeling Podoplanin / gp36 with ab205333 at 0.5 µg in 100 µl dilution.
ab205333 has been referenced in 1 publication.
- Wu S et al. A Dual Targeting Magnetic Nanoparticle for Human Cancer Detection. Nanoscale Res Lett 14:228 (2019). PubMed: 31289961