FITC Anti-Podoplanin / gp36 antibody [18H5] (ab205333)

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Flow Cytometry - FITC Anti-Podoplanin / gp36 antibody [18H5] (ab205333)
  • Flow Cytometry - FITC Anti-Podoplanin / gp36 antibody [18H5] (ab205333)

Key features and details

  • FITC Mouse monoclonal [18H5] to Podoplanin / gp36
  • Suitable for: Flow Cyt
  • Reacts with: Human
  • Conjugation: FITC. Ex: 493nm, Em: 528nm
  • Isotype: IgG1

Overview

  • Product name

    FITC Anti-Podoplanin / gp36 antibody [18H5]
    See all Podoplanin / gp36 primary antibodies

  • Description

    FITC Mouse monoclonal [18H5] to Podoplanin / gp36

  • Host species

    Mouse

  • Conjugation

    FITC. Ex: 493nm, Em: 528nm

  • Tested applications

    Suitable for: Flow Cytmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Tissue, cells or virus corresponding to Human Podoplanin/ gp36. NP_001006625.1. MDCK cells stably expressing Podoplanin/gp36 protein isoform 6.
    Database link: Q86YL7-6

  • Positive control

    • Primary human dermal lymphatic endothelial cells (HDLEC).

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab205333 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

The suggested use of the antibody is ≤ 0.5 μg in 100 μl volume. It is recommended that the conjugate antibody should be titrated for optimal performance for each application.

Target

  • Function

    May be involved in cell migration and/or actin cytoskeleton organization. When expressed in keratinocytes, induces changes in cell morphology with transfected cells showing an elongated shape, numerous membrane protrusions, major reorganization of the actin cytoskeleton, increased motility and decreased cell adhesion. Required for normal lung cell proliferation and alveolus formation at birth. Induces platelet aggregation. Does not have any effect on folic acid or amino acid transport. Does not function as a water channel or as a regulator of aquaporin-type water channels.

  • Tissue specificity

    Highly expressed in placenta, lung, skeletal muscle and brain. Weakly expressed in brain, kidney and liver. In placenta, expressed on the apical plasma membrane of endothelium. In lung, expressed in alveolar epithelium. Up-regulated in colorectal tumors and expressed in 25% of early oral squamous cell carcinomas.

  • Sequence similarities

    Belongs to the podoplanin family.

  • Post-translational
    modifications

    Extensively O-glycosylated. Contains sialic acid residues. O-glycosylation is necessary for platelet aggregation activity.
    The N-terminus is blocked.

  • Cellular localization

    Membrane. Cell projection > filopodium membrane. Cell projection > lamellipodium membrane. Cell projection > microvillus membrane. Cell projection > ruffle membrane. Localized to actin-rich microvilli and plasma membrane projections such as filopodia, lamellipodia and ruffles.
  • Information by UniProt

  • Database links

    • Alternative names

      • Aggrus antibody
      • Glycoprotein 36 KD antibody
      • Glycoprotein 36 antibody
      • gp 36 antibody
      • GP 38 antibody
      • GP 40 antibody
      • gp36 antibody
      • GP38 antibody
      • GP40 antibody
      • HT1A 1 antibody
      • HT1A1 antibody
      • hT1alpha 1 antibody
      • hT1alpha 2 antibody
      • hT1alpha1 antibody
      • hT1alpha2 antibody
      • Lung type I cell membrane associated glycoprotein antibody
      • Lung type I cell membrane associated glycoprotein isoform a antibody
      • Lung type I cell membrane associated glycoprotein T1A 2 antibody
      • OTS 8 antibody
      • OTS8 antibody
      • OTTHUMP00000009640 antibody
      • OTTHUMP00000044504 antibody
      • PA2.26 antibody
      • PA2.26 antigen antibody
      • Pdpn antibody
      • PDPN_HUMAN antibody
      • Podoplanin antibody
      • PSEC0003 antibody
      • PSEC0025 antibody
      • T1 alpha antibody
      • T1 ALPHA GENE antibody
      • T1-alpha antibody
      • T1A 2 antibody
      • T1A antibody
      • TI1A antibody
      • TIA 2 antibody
      • TIA2 antibody
      see all

    Images

    • Flow Cytometry - FITC Anti-Podoplanin / gp36 antibody [18H5] (ab205333)
      Flow Cytometry - FITC Anti-Podoplanin / gp36 antibody [18H5] (ab205333)

      Flow cytometric analysis of primary Human dermal lymphatic endothelial cells (HDLEC)  labeling Podoplanin / gp36 with ab205333 at 0.5 µg in 100 µl dilution (lower panel) compared to negative control cells containing no primary antibody (upper panel) or conjugated secondary antibody alone (middle panel). 

    • Flow Cytometry - FITC Anti-Podoplanin / gp36 antibody [18H5] (ab205333)
      Flow Cytometry - FITC Anti-Podoplanin / gp36 antibody [18H5] (ab205333)

      Flow cytometric analysis of primary Human dermal lymphatic endothelial cells (HDLEC) labeling Podoplanin / gp36 with ab205333 at 0.5 µg in 100 µl dilution.  

    References (1)

    Publishing research using ab205333? Please let us know so that we can cite the reference in this datasheet.

    ab205333 has been referenced in 1 publication.

    • Wu S  et al. A Dual Targeting Magnetic Nanoparticle for Human Cancer Detection. Nanoscale Res Lett 14:228 (2019). PubMed: 31289961

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