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    fitc-pr3-antibody-wgm2-ab91182.pdf

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Signal Transduction Cytoskeleton / ECM Basal Lamina
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FITC Anti-PR3 antibody [WGM2] (ab91182)

  • Datasheet
  • SDS
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Flow Cytometry - FITC Anti-PR3 antibody [WGM2] (ab91182)

    Key features and details

    • FITC Mouse monoclonal [WGM2] to PR3
    • Suitable for: Flow Cyt
    • Reacts with: Human
    • Conjugation: FITC. Ex: 493nm, Em: 528nm
    • Isotype: IgG1

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    Overview

    • Product name

      FITC Anti-PR3 antibody [WGM2]
      See all PR3 primary antibodies
    • Description

      FITC Mouse monoclonal [WGM2] to PR3
    • Host species

      Mouse
    • Conjugation

      FITC. Ex: 493nm, Em: 528nm
    • Tested applications

      Suitable for: Flow Cytmore details
    • Species reactivity

      Reacts with: Human
    • Immunogen

      neutrophils (degranulated supernatant)human

    • General notes


      Blocks PR3 activity and partially inhibits the binding of human PR3-ANCA to PR3.

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C.
    • Storage buffer

      Preservative: 0.02% Sodium azide
      Constituents: PBS, 0.1% BSA
    • Concentration information loading...
    • Purity

      Protein G purified
    • Purification notes

      0.2 µm filtered antibody solution
    • Primary antibody notes

      Blocks PR3 activity and partially inhibits the binding of human PR3-ANCA to PR3.
    • Clonality

      Monoclonal
    • Clone number

      WGM2
    • Isotype

      IgG1
    • Research areas

      • Signal Transduction
      • Cytoskeleton / ECM
      • Basal Lamina
      • Signal Transduction
      • Cytoskeleton / ECM
      • Extracellular Matrix
      • ECM Proteins
      • Collagen
      • Signal Transduction
      • Cytoskeleton / ECM
      • Extracellular Matrix
      • ECM Enzymes
      • Other Enzymes

    Associated products

    • Alternative Versions

      • Anti-PR3 antibody [WGM2] (ab91181)
    • Isotype control

      • FITC Mouse IgG1 [B11/6] - Isotype Control (ab91356)
    • Recombinant Protein

      • Recombinant Human PR3 protein (ab153798)

    Applications

    Our Abpromise guarantee covers the use of ab91182 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt Use 5µl for 106 cells.

    ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

     

    Target

    • Function

      Polymorphonuclear leukocyte serine protease that degrades elastin, fibronectin, laminin, vitronectin, and collagen types I, III, and IV (in vitro) and causes emphysema when administered by tracheal insufflation to hamsters.
    • Sequence similarities

      Belongs to the peptidase S1 family. Elastase subfamily.
      Contains 1 peptidase S1 domain.
    • Target information above from: UniProt accession P24158 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 5657 Human
      • Omim: 177020 Human
      • SwissProt: P24158 Human
      • Unigene: 928 Human
      • Alternative names

        • ACPA antibody
        • AGP 7 antibody
        • AGP7 antibody
        • AGP7 serine proteinase antibody
        • Azurophil Granule Protein 7 antibody
        • C ANCA antibody
        • C ANCA antigen antibody
        • C-ANCA antigen antibody
        • CANCA antibody
        • EC 3.4.21.76 antibody
        • Leukocyte proteinase 3 antibody
        • MBN antibody
        • MBT antibody
        • MBT WEGENER AUTOANTIGEN antibody
        • Myeloblastin antibody
        • Neutrophil proteinase 4 antibody
        • NP 4 antibody
        • NP-4 antibody
        • NP4 antibody
        • P29 antibody
        • PR 3 antibody
        • PR-3 antibody
        • PR3 antibody
        • Proteinase 3 antibody
        • Proteinase3 antibody
        • PRTN 3 antibody
        • Prtn3 antibody
        • PRTN3_HUMAN antibody
        • Serine proteinase neutrophil Wegener granulomatosis autoantigen antibody
        • Serine proteinase, neutrophil antibody
        • Wegener autoantigen antibody
        • Wegener granulomatosis autoantigen antibody
        see all

      Images

      • Flow Cytometry - FITC Anti-PR3 antibody [WGM2] (ab91182)
        Flow Cytometry - FITC Anti-PR3 antibody [WGM2] (ab91182)
        Overlay histogram showing peripheral blood lymphocytes cells stained with ab91182 (red line). The cells were fixed with 4% paraformaldehydel (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab91182, 0.5µg/1x106 cells) for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 (1µg/1x106 cells ). Acquisition of >5,000 events was performed.

      Protocols

      • Flow cytometry protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (1)

      Publishing research using ab91182? Please let us know so that we can cite the reference in this datasheet.

      ab91182 has been referenced in 1 publication.

      • Thom SR  et al. Inflammatory responses to acute elevations of carbon dioxide in mice. J Appl Physiol (1985) 123:297-302 (2017). PubMed: 28495847

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