FITC Anti-PR3 antibody [WGM2] (ab91182)
![Flow Cytometry - FITC Anti-PR3 antibody [WGM2] (ab91182)](https://www.abcam.com/ps/products/91/ab91182/Images/ab91182-106750-anti-pr3-antibody-wgm2-fitc-flow-cytometry.jpg "Flow Cytometry - FITC Anti-PR3 antibody [WGM2] (ab91182)")
Key features and details
- FITC Mouse monoclonal [WGM2] to PR3
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG1
Overview
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Product name
FITC Anti-PR3 antibody [WGM2]
See all PR3 primary antibodies -
Description
FITC Mouse monoclonal [WGM2] to PR3 -
Host species
Mouse -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
neutrophils (degranulated supernatant)human
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General notes
Blocks PR3 activity and partially inhibits the binding of human PR3-ANCA to PR3.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS, 0.1% BSA -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
0.2 µm filtered antibody solution -
Primary antibody notes
Blocks PR3 activity and partially inhibits the binding of human PR3-ANCA to PR3. -
Clonality
Monoclonal -
Clone number
WGM2 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab91182 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use 5µl for 106 cells.
ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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| Notes |
|---|
|
Flow Cyt
Use 5µl for 106 cells. ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|
Target
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Function
Polymorphonuclear leukocyte serine protease that degrades elastin, fibronectin, laminin, vitronectin, and collagen types I, III, and IV (in vitro) and causes emphysema when administered by tracheal insufflation to hamsters. -
Sequence similarities
Belongs to the peptidase S1 family. Elastase subfamily.
Contains 1 peptidase S1 domain. - Information by UniProt
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Database links
- Entrez Gene: 5657 Human
- Omim: 177020 Human
- SwissProt: P24158 Human
- Unigene: 928 Human
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Alternative names
- ACPA antibody
- AGP 7 antibody
- AGP7 antibody
see all
Images
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Flow Cytometry - FITC Anti-PR3 antibody [WGM2] (ab91182)Overlay histogram showing peripheral blood lymphocytes cells stained with ab91182 (red line). The cells were fixed with 4% paraformaldehydel (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab91182, 0.5µg/1x106 cells) for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 (1µg/1x106 cells ). Acquisition of >5,000 events was performed.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab91182 has been referenced in 1 publication.
- Thom SR et al. Inflammatory responses to acute elevations of carbon dioxide in mice. J Appl Physiol (1985) 123:297-302 (2017). PubMed: 28495847