FITC Anti-PR3 antibody [WGM2] (ab91182)
![Flow Cytometry - FITC Anti-PR3 antibody [WGM2] (ab91182)](https://www.abcam.com/ps/products/91/ab91182/Images/ab91182-106750-anti-pr3-antibody-wgm2-fitc-flow-cytometry.jpg "Flow Cytometry - FITC Anti-PR3 antibody [WGM2] (ab91182)")
Key features and details
- FITC Mouse monoclonal [WGM2] to PR3
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG1
Overview
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Product name
FITC Anti-PR3 antibody [WGM2]
See all PR3 primary antibodies -
Description
FITC Mouse monoclonal [WGM2] to PR3 -
Host species
Mouse -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
neutrophils (degranulated supernatant)human
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General notes
Blocks PR3 activity and partially inhibits the binding of human PR3-ANCA to PR3.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS, 0.1% BSA -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
0.2 µm filtered antibody solution -
Primary antibody notes
Blocks PR3 activity and partially inhibits the binding of human PR3-ANCA to PR3. -
Clonality
Monoclonal -
Clone number
WGM2 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab91182 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use 5µl for 106 cells. ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|
Target
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Function
Polymorphonuclear leukocyte serine protease that degrades elastin, fibronectin, laminin, vitronectin, and collagen types I, III, and IV (in vitro) and causes emphysema when administered by tracheal insufflation to hamsters. -
Sequence similarities
Belongs to the peptidase S1 family. Elastase subfamily.
Contains 1 peptidase S1 domain. - Information by UniProt
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Database links
- Entrez Gene: 5657 Human
- Omim: 177020 Human
- SwissProt: P24158 Human
- Unigene: 928 Human
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Alternative names
- ACPA antibody
- AGP 7 antibody
- AGP7 antibody
see all
Images
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Flow Cytometry - FITC Anti-PR3 antibody [WGM2] (ab91182)Overlay histogram showing peripheral blood lymphocytes cells stained with ab91182 (red line). The cells were fixed with 4% paraformaldehydel (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab91182, 0.5µg/1x106 cells) for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 (1µg/1x106 cells ). Acquisition of >5,000 events was performed.
References (1)
ab91182 has been referenced in 1 publication.
- Thom SR et al. Inflammatory responses to acute elevations of carbon dioxide in mice. J Appl Physiol (1985) 123:297-302 (2017). PubMed: 28495847
