FITC Anti-PSGL-1 antibody [TC2] (ab88033)
![Flow Cytometry - FITC Anti-PSGL-1 antibody [TC2] (ab88033)](https://www.abcam.com/ps/products/88/ab88033/Images/ab88033-102947-anti-psgl-1-antibody-tc2-fitc-flow-cytometry.jpg "Flow Cytometry - FITC Anti-PSGL-1 antibody [TC2] (ab88033)")
Key features and details
- FITC Mouse monoclonal [TC2] to PSGL-1
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG1
Overview
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Product name
FITC Anti-PSGL-1 antibody [TC2]
See all PSGL-1 primary antibodies -
Description
FITC Mouse monoclonal [TC2] to PSGL-1 -
Host species
Mouse -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Specificity
Reacts with PSGL-1, a 220 kDa type I integral membrane protein expressed as disulfide-linked homodimer (sialomucin family). PSGL-1 is present on the most peripheral blood T lymphocytes, monocytes, granulocytes; it is also expressed on a subpopulation of B lymphocytes and CD34+ bone marrow cells. -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to PSGL-1. Human thymocytes.
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Positive control
- Human peripheral blood
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General notes
The purified antibody(>95% by SDS-PAGE) is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.2% BSA
0.2% BSA (high-grade, protease free) -
Concentration information loading... -
Purity
Size exclusion -
Purification notes
Size exclusion chromatography -
Clonality
Monoclonal -
Clone number
TC2 -
Isotype
IgG1 -
Research areas
Associated products
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab88033 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use 20µl for 106 cells. ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|
Target
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Function
A SLe(x)-type proteoglycan, which through high affinity, calcium-dependent interactions with E-, P- and L-selectins, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation. Critical for the initial leukocyte capture.
(Microbial infection) Acts as a receptor for enterovirus 71. -
Tissue specificity
Expressed on neutrophils, monocytes and most lymphocytes. -
Post-translational
modificationsDisplays complex, core-2, sialylated and fucosylated O-linked oligosaccharides, at least some of which appear to contain poly-N-acetyllactosamine with varying degrees of substitution. Mainly disialylated or neutral forms of the core-2 tetrasaccharide, Galbeta1-->4GlcNAcbeta1-->6(Galbeta1-->3)GalNAcOH. The GlcN:GalN ratio is approximately 2:1 and the Man:Fuc ratio 3:5. Contains about 14% fucose with alpha-1,3 linkage present in two forms: One species is a disialylated, monofucosylated glycan, and the other, a monosialylated, trifucosylated glycan with a polylactosamine backbone. The fucosylated forms carry the Lewis antigen and are important for interaction with selectins and for functioning in leukocyte rolling. The modification containing the sialyl Lewis X glycan is on Thr-57. No sulfated O-glycans. Some N-glycosylation.
Sulfation, in conjunction with the SLe(x)-containing glycan, is necessary for P- and L-selectin binding. High affinity P-selectin binding has a preferred requirement for the isomer sulfated on both Tyr-48 and Tyr-51, whereas L-selectin binding requires predominantly sulfation on Tyr-51 with sulfation on Tyr-48 playing only a minor role. These sulfations play an important role in L- and P-selectin-mediated neutrophil recruitment, and leukocyte rolling. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 6404 Human
- Omim: 600738 Human
- SwissProt: Q14242 Human
- Unigene: 591014 Human
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Alternative names
- CD 162 antibody
- CD162 antibody
- CD162 antigen antibody
see all
Images
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Flow Cytometry - FITC Anti-PSGL-1 antibody [TC2] (ab88033)
ah88033 detecting PSGL-1 in Human peripheral blood cells by Flow cytometry
References (0)
ab88033 has not yet been referenced specifically in any publications.
