FITC Rat IgG2a, kappa monoclonal [RTK2758] - Isotype control (ab18446)

Q&A (3)References (6)

Overview

  • Product name

    FITC Rat IgG2a, kappa monoclonal [RTK2758] - Isotype control
    See all Rat isotype controls

  • Conjugation

    FITC. Ex: 493nm, Em: 528nm

  • Specificity

    ab18446 RTK2758 immunoglobulin reacts with KLH. The RTK2758 immunoglobulin was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse and human tissues

  • Tested applications

    Suitable for: Flow Cytmore details

  • General notes

    ab18446 RTK2758 immunoglobulin is useful as an isotype-matched control for Western blotting, immunoprecipitation, immunohistochemical staining, bioassay and immunofluorescent staining (surface or intracellular) for flow cytometric analysis.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    pH: 7.20
    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • Purity

    Affinity purified

  • Purification notes

    The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.

  • Isotype control notes

    ab18446 RTK2758 immunoglobulin is useful as an isotype-matched control for Western blotting, immunoprecipitation, immunohistochemical staining, bioassay and immunofluorescent staining (surface or intracellular) for flow cytometric analysis.

  • Clonality

    Monoclonal

  • Clone number

    RTK2758

  • Isotype

    IgG2a

  • Light chain type

    kappa

  • Research areas

  • Alternative names

    • Rat Isotype Control

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab18446 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
Use at an assay dependent concentration.

Suitable for surface or intracellular staining.
Notes
Flow Cyt
Use at an assay dependent concentration.

Suitable for surface or intracellular staining.

References (6)

Publishing research using ab18446? Please let us know so that we can cite the reference in this datasheet.

ab18446 has been referenced in 6 publications.

  • Guo X  et al. Hepatitis C virus infection induces endoplasmic reticulum stress and apoptosis in human fetal liver stem cells. J Pathol N/A:N/A (2019). PubMed: 30680725
  • Thapa P  et al. The differentiation of ROR-?t expressing iNKT17 cells is orchestrated by Runx1. Sci Rep 7:7018 (2017). PubMed: 28765611
  • Xia X  et al. Inhibitor of DNA binding 1 regulates cell cycle progression of endothelial progenitor cells through induction of Wnt2 expression. Mol Med Rep N/A:N/A (2016). PubMed: 27432753
  • Kjær I  et al. Cetuximab Resistance in Squamous Carcinomas of the Upper Aerodigestive Tract Is Driven by Receptor Tyrosine Kinase Plasticity: Potential for mAb Mixtures. Mol Cancer Ther 15:1614-26 (2016). PubMed: 27196767
  • Guo Z  et al. Combined Trabectedin and anti-PD1 antibody produces a synergistic antitumor effect in a murine model of ovarian cancer. J Transl Med 13:247 (2015). IF ; Mouse . PubMed: 26219551
  • Zeng SL  et al. Mesenchymal stem cells abrogate experimental asthma by altering dendritic cell function. Mol Med Rep 12:2511-20 (2015). Mouse . PubMed: 25936350

Customer reviews and Q&As

Q&A
Submit a question

1-3 of 3 Q&A

Question

I have a question regarding the following product that I bought:
Rat IgG2a, kappa monoclonal [RTK2758] (FITC) - Isotype control
Cat. Nr.: ab18446
At with concentration is sailed this antibody?
This is important to understand some strange results I´m having in FACS analysis.
Please let me know a.s.a.p. this information
Thank you in advance

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Answer

Thank you for contacting us.
As shown on the product datasheet, www.abcam.com/ab18446, this product is a whole antiserum.
Unpurified antibodies will not have a concentration stated on the datasheet. For whole antiserum (such as ab18446), culture supernatant or ascites fluid products the concentration has not been determined. Unpurified antibody preparations vary significantly in specific antibody concentration. One may refer to the “concentration estimate” area in the following link as a guideline for estimation: https://www.abcam.com/index.html?pageconfig=technicalfaqs#8. (Concentration estimate: 1-10 mg/ml for Whole antiserum).
Please remember that these dilutions and concentration estimates are simply recommended as a starting point, and it may be necessary to adjust the dilution based on the experimental results.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question

Product code: 11400
Inquiry: Hi, I am looking for an antibody against EGFR (ErbB1) to use on human and mouse cell lines for FACS with a FITC label and I have found ICR10. I was wondering if you could tell me which antibody to buy to use an an Isotope control (rat) with this antibody? Mnay thanks in advance

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Answer

Thank you for contacting us.

The only isotype control we can offer is ab18446; https://www.abcam.com/Rat-IgG2a-kappa-monoclonal-RTK2758-FITC-Isotype-control-ab18446.html

Please note that the concentration of isotype is not known because it is not a purified antibody. When using along with ab11400 you will need to optimize the dilution of antibody for better results.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Question

Purchased wrong antibody ab18703 and ab18450. How could we return the antibodies? We are interested in getting ab18446 and rat monoclonal anti IgG2c antibody which we could not find in catalogue.

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Answer

Thank you for your phone call. I can confirm that you can send the products back to us. When you return the goods, please ensure that you follow the procedures as below: - Return in the original Abcam box with the cooler box and ice pack (or similar) within 7 days from today. - Return all documents (or copies of) that arrived with the product (if they’re available – should be invoice, delivery note and datasheet). Please note that we will be unable to issue the credit note if the product is dispatched without the cooler box. However we are not able to accept products returned on dry ice. - Please mark on the documents the reason of return and Ref: - Please return using an over night courier. - You will need to provide the courier with an AirWay Bill – which you can get from the courier, and fill out with our details. - Our address is: Abcam plc, 330 Cambridge Science Park, Milton Road, Cambridge, CB4 0FL When we have received the goods, we will process the return. We will then raise a credit note for the invoice less 20% restocking fee. If you have already paid this invoice, we will reallocate this money to a new invoice for a future order and if there’s any difference in price, we will refund or re-invoice accordingly. For the correct item, please place a new order in the normal methods. Please confirm if you are planning to return the product to us. Important: if the product is not returned to Abcam within next 7 days we will cancel the credit note. PS: We have anti IgG2a and anti IgG2c available in catalogue that are currently in stock. These 2 antibodies exactly match your request. The catalogue numbers are: ab18446 and ab114134

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