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FITC Anti-RFP antibody (ab34764)

Reviews (1)Q&A (6)References (6)
Western blot - FITC Anti-RFP antibody (ab34764)

    Key features and details

    • FITC Rabbit polyclonal to RFP
    • Suitable for: ELISA, ICC/IF, Flow Cyt, WB
    • Reacts with: Human
    • Conjugation: FITC. Ex: 493nm, Em: 528nm
    • Isotype: IgG

    Overview

    • Product name

      FITC Anti-RFP antibody
      See all RFP primary antibodies

    • Description

      FITC Rabbit polyclonal to RFP

    • Host species

      Rabbit

    • Conjugation

      FITC. Ex: 493nm, Em: 528nm

    • Specificity

      ab34764 detects RFP tagged proteins. No reaction was observed against serum proteins.Expect reactivity against RFP and its variants: mCherry, tdTomato, mBanana, mOrange, mPlum, mOrange and mStrawberry”.

    • Tested applications

      Suitable for: ELISA, ICC/IF, Flow Cyt, WBmore details

    • Species reactivity

      Reacts with: Human

    • Immunogen

      Fusion protein corresponding to RFP. derived from the mushroom polyp coral Discosoma

    • General notes

      3.4 moles FITC per mole of rabbit IgG.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Store at +4°C.

    • Storage buffer

      Preservative: 0.01% Sodium azide
      Constituents: 1% BSA, 0.42% Potassium phosphate, 0.87% Sodium chloride
    • Concentration information loading...

    • Purity

      Immunogen affinity purified

    • Purification notes

      affinity purification was followed by solid phase adsorption to remove any unwanted reactivities.

    • Clonality

      Polyclonal

    • Isotype

      IgG

    • Research areas

    Associated products

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab34764 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ELISA
    Use at an assay dependent concentration.
    ICC/IF (1)
    Use at an assay dependent concentration.
    Flow Cyt
    Use at an assay dependent concentration.

    ab37406 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
    WB
    1/10000. Detects a band of approximately 27 kDa.
    Notes
    ELISA
    Use at an assay dependent concentration.
    ICC/IF
    Use at an assay dependent concentration.
    Flow Cyt
    Use at an assay dependent concentration.

    ab37406 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
    WB
    1/10000. Detects a band of approximately 27 kDa.

    Target

    • Relevance

      Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions.

    • Alternative names

      • DsRed antibody
      • GFP like chromoprotein antibody
      • red fluorescent protein antibody
      • RFP antibody

    Images

    • Western blot - FITC Anti-RFP antibody (ab34764)
      Western blot - FITC Anti-RFP antibody (ab34764)
      All lanes : FITC Anti-RFP antibody (ab34764) at 1/2500 dilution

      Lane 1 : HeLa cell extract
      Lane 2 : HeLa lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat-a-Rabbit IgG [H&L] at 1/5000 dilution

    References (6)

    Publishing research using ab34764? Please let us know so that we can cite the reference in this datasheet.

    ab34764 has been referenced in 6 publications.

    • Sidwell T  et al. Attenuation of TCR-induced transcription by Bach2 controls regulatory T cell differentiation and homeostasis. Nat Commun 11:252 (2020). PubMed: 31937752
    • Saxena V  et al. Whole Transcriptome Analysis of Renal Intercalated Cells Predicts Lipopolysaccharide Mediated Inhibition of Retinoid X Receptor alpha Function. Sci Rep 9:545 (2019). PubMed: 30679625
    • Ruelas Cinco EDC  et al. Expression of the receptor of advanced glycation end-products (RAGE) and membranal location in peripheral blood mononuclear cells (PBMC) in obesity and insulin resistance. Iran J Basic Med Sci 22:623-630 (2019). PubMed: 31231489
    • Donnelly JM  et al. Sonic hedgehog mediates the proliferation and recruitment of transformed mesenchymal stem cells to the stomach. PLoS One 8:e75225 (2013). PubMed: 24069395
    • Starremans PG  et al. A mouse model for polycystic kidney disease through a somatic in-frame deletion in the 5' end of Pkd1. Kidney Int 73:1394-405 (2008). PubMed: 18385665
    • Carter V  et al. PbSR is synthesized in macrogametocytes and involved in formation of the malaria crystalloids. Mol Microbiol 68:1560-9 (2008). WB . PubMed: 18452513

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-7 of 7 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-RFP antibody (FITC)

     Excellent
    Abreviews
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Xenopus laevis Cell (whole embryo)
    Specification
    whole embryo
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - PBT
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Ms. Vanja Stankic

    Verified customer

    Submitted Jan 22 2010

    Question

    Is it known whether this antibody will cross react with BFP (the point mutant of RFP) or GFP?

    Read More
    Answer

    We have not yet tested the FITC conjugated anti RFP antibody against GFP, but this antibody did not show significant amounts of specific binding to BFP.

    Read More

    Question

    thank you very much for your email. We would like to make use of your
    offer regarding the AbTrial for the RFP (Biotin) antibody ab34771.
    Just to understand this correctly, when we order the antibody now we
    don't need to mention the code you will send us. Only when we order
    something after submitting the Abreview we will need to provide it in
    order to get the discount of 410 Euros. Is that correct?
    Thank you very much for your help.
    Best regards,

    Read More
    Answer

    DISCOUNT CODE: ABTRIAL-xxxxx
    Expiration date: 04/04/12
    Value: €410.00

    I am very pleased to hear you would like to accept our offer and test ab34771 in Flow Cytometry. This code will give you €410.00 off your next order before the expiration date. To redeem this offer, please submit an Abreview for Flow cytometry and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
    For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


    It is correct, that you do not have to mention the code with your order of ab34771. The code will be activated by submitting the Abreview and needs to be mentioned with the next order.

    Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here: www.abcam.com/abtrial.

    Read More

    Question

    I am a colleague of Nicole and we would both work with the RFP
    antibody, in case we are going to order one.
    Firstly, we would like to know, if the AbTrail offer also applies on
    the biotinylated Anti-RFP antibody (ab34771)? We would use it in flow
    cytometry with some secondary streptavidin antibody. Secondly, what
    would be the value of the discount for the next order, if we go for
    the AbTrial option?
    Thanks a lot for your help!
    Best regards,

    Read More
    Answer

    Thank you for your answer.

    I would be pleased to offer you the AbTrial for the ab34771 to use this antibody in FlowCytometry. If you decide to order this antibody please let me now in advance so that I can provide you with the code.

    You will receive the whole value of the antibody you are testing to order a new product. That means, if you decide to test the ab34771 you will receive a code with a value of €410.00. The code will be activated after you submit the Abreview.

    Please let me know about your decision. I look forward hearing from you.

    Read More

    Question

    Hi,
    I'm looking for an anti-mRFP antibody that is suitable to be used in
    flow cytometry. I saw that you have one that is FITC conjugated (#
    ab34764) but unfortunately I also have GFP expression in my cells and
    can't use this one. Do you use the same antibody clone for the
    biotinylated anti-RFP antibody (ab34771)? So maybe it would be
    possible to use this one? Do you have any information whether it could
    work in flow cytometry or would it even be possible to get a small
    sample to test this? Of cause you could also offer other suitable
    anti-mRFP antibodies.
    Thanks and kind regards

    Read More
    Answer

    Thank you for contacting us.

    In this case I would recommend to use the Anti-RFP antibody (ab62341). If it is suitable for your experiment you could use a secondary antibody to detect the Anti RFP antibody.

    If the conjugate needs to be on the primary antibody I would recommend to have a look at our conjugation kits https://www.abcam.com/index.html?pageconfig=resource&rid=13148.

    Because the Anti-RFP antibody (ab62341) has not been tested for flow cytometry I would am pleased to offer you the AbTrial for the use of this product in flow cytometry. By participating in our AbTrial program you can use our products in an untested application or species without financial risk.

    Simply follow these easy steps below to apply for our AbTrial Program:

    1. Reply to this email, letting me know you are interested in testing this product.

    2. I will email you an inactive personal discount code for the value of the product.

    3. Purchase and test the product at the regular price.

    4. Submit your results, including your discount code in the additional notes section of your Abreview.

    5. Once the Abreview is submitted, the discount code will become active.

    6. Apply your discount code on your next order to receive that value off.

    Please let me know if you have any questions about this offer and I would be happy to help you further.

    The Terms and Conditions of this offer can be found at: www.abcam.com/abtrial.

    I hope I could be of help and I look forward hearing from you about your decision regarding the AbTrial offer.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

    Read More

    Question

    I would like to know if your antibody Anti-RFP antibody (FITC) (ab34764) is suitable to detect the DsRed Express (or alternatively named DsRed.T1) for flow cytometry.
    Thank you in advance.

    Best regards

    Read More
    Answer

    Thank you for your enquiry.

    This Our RFP antibody isdesigned to detect all RFP variants from Discosoma. I am sorry we do not have specific data for the the use of this product with DS Red express in Flow Cytometry, however in theory it would be expected to work well.

    I hope this will be helpful. If you have any further questions, please do not hesitate to contact us.

    Read More

    Question

    Inquiry: Please advise if the antibody recognize mRFP1

    Read More
    Answer

    Thank you for contacting us with your question.

    We have not specifically tested the antibody against mRFP1, but it is designed to react with RFP variants such as mRFP1. If you do have any problems using this antibody with mRPF1, please let me know as it will be covered under our Abpromise guarantee.

    I hope this information will be useful, but please let me know if you have any further questions or if there is anything else that we can do for you.

    Read More

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