FITC Anti-RFP antibody (ab34764)
Key features and details
- FITC Rabbit polyclonal to RFP
- Suitable for: ELISA, ICC/IF, Flow Cyt, WB
- Reacts with: Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG
Overview
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Product name
FITC Anti-RFP antibody
See all RFP primary antibodies -
Description
FITC Rabbit polyclonal to RFP -
Host species
Rabbit -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Specificity
ab34764 detects RFP tagged proteins. No reaction was observed against serum proteins.Expect reactivity against RFP and its variants: mCherry, tdTomato, mBanana, mOrange, mPlum, mOrange and mStrawberry”. -
Tested applications
Suitable for: ELISA, ICC/IF, Flow Cyt, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Fusion protein corresponding to RFP. derived from the mushroom polyp coral Discosoma
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General notes
3.4 moles FITC per mole of rabbit IgG.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 1% BSA, 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
affinity purification was followed by solid phase adsorption to remove any unwanted reactivities. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab34764 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
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| ICC/IF | (1) |
Use at an assay dependent concentration.
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| Flow Cyt |
Use at an assay dependent concentration.
ab37406 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
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| WB |
1/10000. Detects a band of approximately 27 kDa.
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| Notes |
|---|
|
ELISA
Use at an assay dependent concentration. |
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ICC/IF
Use at an assay dependent concentration. |
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Flow Cyt
Use at an assay dependent concentration. ab37406 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/10000. Detects a band of approximately 27 kDa. |
Target
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Relevance
Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. -
Alternative names
- DsRed antibody
- GFP like chromoprotein antibody
- red fluorescent protein antibody
- RFP antibody
Images
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Western blot - FITC Anti-RFP antibody (ab34764)All lanes : FITC Anti-RFP antibody (ab34764) at 1/2500 dilution
Lane 1 : HeLa cell extract
Lane 2 : HeLa lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat-a-Rabbit IgG [H&L] at 1/5000 dilution
Datasheets and documents
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Datasheet download
References (6)
ab34764 has been referenced in 6 publications.
- Sidwell T et al. Attenuation of TCR-induced transcription by Bach2 controls regulatory T cell differentiation and homeostasis. Nat Commun 11:252 (2020). PubMed: 31937752
- Saxena V et al. Whole Transcriptome Analysis of Renal Intercalated Cells Predicts Lipopolysaccharide Mediated Inhibition of Retinoid X Receptor alpha Function. Sci Rep 9:545 (2019). PubMed: 30679625
- Ruelas Cinco EDC et al. Expression of the receptor of advanced glycation end-products (RAGE) and membranal location in peripheral blood mononuclear cells (PBMC) in obesity and insulin resistance. Iran J Basic Med Sci 22:623-630 (2019). PubMed: 31231489
- Donnelly JM et al. Sonic hedgehog mediates the proliferation and recruitment of transformed mesenchymal stem cells to the stomach. PLoS One 8:e75225 (2013). PubMed: 24069395
- Starremans PG et al. A mouse model for polycystic kidney disease through a somatic in-frame deletion in the 5' end of Pkd1. Kidney Int 73:1394-405 (2008). PubMed: 18385665
- Carter V et al. PbSR is synthesized in macrogametocytes and involved in formation of the malaria crystalloids. Mol Microbiol 68:1560-9 (2008). WB . PubMed: 18452513
