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Signal Transduction Growth Factors/Hormones Hormones
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FITC Anti-ROR gamma antibody [4G419], prediluted (ab104906)

  • Datasheet
  • SDS
Reviews (1)Q&A (1)References (1)

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Flow Cytometry - FITC Anti-ROR gamma antibody [4G419], prediluted (ab104906)

    Key features and details

    • FITC Mouse monoclonal [4G419] to ROR gamma, prediluted
    • Suitable for: Flow Cyt
    • Reacts with: Human
    • Conjugation: FITC. Ex: 493nm, Em: 528nm
    • Isotype: IgG1

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    Overview

    • Product name

      FITC Anti-ROR gamma antibody [4G419], prediluted
      See all ROR gamma primary antibodies
    • Description

      FITC Mouse monoclonal [4G419] to ROR gamma, prediluted
    • Host species

      Mouse
    • Conjugation

      FITC. Ex: 493nm, Em: 528nm
    • Specificity

      This antibody will detect ROR gamma and may detect ROR gammat, but can not be used to distinguish between the two proteins.
    • Tested Applications & Species

      Application Species
      Flow Cyt
      Human
      See all applications and species data
    • Immunogen

      Synthetic peptide, corresponding to a region within N terminal amino acids 1-50 of Human ROR gamma.

    • Positive control

      • Human lymphocytes

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C.
    • Storage buffer

      Preservative: 0.05% Sodium azide
      Constituents: 0.05% BSA, 0.87% Sodium chloride, 0.121% Tris
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Monoclonal
    • Clone number

      4G419
    • Isotype

      IgG1
    • Light chain type

      kappa
    • Research areas

      • Signal Transduction
      • Growth Factors/Hormones
      • Hormones
      • Epigenetics and Nuclear Signaling
      • Nuclear Signaling Pathways
      • Nuclear Receptors
      • Corticoid
      • Epigenetics and Nuclear Signaling
      • Transcription
      • Transcription Factors

    Associated products

    • Alternative Versions

      • PE Anti-ROR gamma antibody [4G419] (ab104950)
    • Isotype control

      • FITC Mouse IgG1 [B11/6] - Isotype Control (ab91356)
    • Recombinant Protein

      • Recombinant Human ROR gamma protein (ab159369)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab104906 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    Flow Cyt
    Human
    All applications
    Rat
    Cow
    Dog
    Application Abreviews Notes
    Flow Cyt (1)
    Use 1µl for 106 cells.

    ab106163 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

     

    Notes
    Flow Cyt
    Use 1µl for 106 cells.

    ab106163 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

     

    Target

    • Function

      Nuclear receptor that binds DNA as a monomer to ROR response elements (RORE) containing a single core motif half-site 5'-AGGTCA-3' preceded by a short A-T-rich sequence. Key regulator of cellular differentiation, immunity, peripheral circadian rhythm as well as lipid, steroid, xenobiotics and glucose metabolism (PubMed:19381306, PubMed:19965867, PubMed:22789990, PubMed:26160376, PubMed:20203100). Considered to have intrinsic transcriptional activity, have some natural ligands like oxysterols that act as agonists (25-hydroxycholesterol) or inverse agonists (7-oxygenated sterols), enhancing or repressing the transcriptional activity, respectively (PubMed:19965867, PubMed:22789990). Recruits distinct combinations of cofactors to target gene regulatory regions to modulate their transcriptional expression, depending on the tissue, time and promoter contexts. Regulates the circadian expression of clock genes such as CRY1, ARNTL/BMAL1 and NR1D1 in peripheral tissues and in a tissue-selective manner. Competes with NR1D1 for binding to their shared DNA response element on some clock genes such as ARNTL/BMAL1, CRY1 and NR1D1 itself, resulting in NR1D1-mediated repression or RORC-mediated activation of the expression, leading to the circadian pattern of clock genes expression. Therefore influences the period length and stability of the clock. Involved in the regulation of the rhythmic expression of genes involved in glucose and lipid metabolism, including PLIN2 and AVPR1A (PubMed:19965867). Negative regulator of adipocyte differentiation through the regulation of early phase genes expression, such as MMP3. Controls adipogenesis as well as adipocyte size and modulates insulin sensitivity in obesity. In liver, has specific and redundant functions with RORA as positive or negative modulator of expression of genes encoding phase I and Phase II proteins involved in the metabolism of lipids, steroids and xenobiotics, such as SULT1E1. Also plays also a role in the regulation of hepatocyte glucose metabolism through the regulation of G6PC and PCK1 (PubMed:19965867). Regulates the rhythmic expression of PROX1 and promotes its nuclear localization (PubMed:19381306, PubMed:19965867, PubMed:22789990, PubMed:26160376, PubMed:20203100). Plays an indispensable role in the induction of IFN-gamma dependent anti-mycobacterial systemic immunity (PubMed:26160376).
      Isoform 2: Essential for thymopoiesis and the development of several secondary lymphoid tissues, including lymph nodes and Peyer's patches. Required for the generation of LTi (lymphoid tissue inducer) cells. Regulates thymocyte survival through DNA-binding on ROREs of target gene promoter regions and recruitment of coactivaros via the AF-2. Also plays a key role, downstream of IL6 and TGFB and synergistically with RORA, for lineage specification of uncommitted CD4(+) T-helper (T(H)) cells into T(H)17 cells, antagonizing the T(H)1 program. Probably regulates IL17 and IL17F expression on T(H) by binding to the essential enhancer conserved non-coding sequence 2 (CNS2) in the IL17-IL17F locus. May also play a role in the pre-TCR activation cascade leading to the maturation of alpha/beta T-cells and may participate in the regulation of DNA accessibility in the TCR-J(alpha) locus.
    • Tissue specificity

      Isoform 1 is widely expressed in many tissues, including liver and adipose, and highly expressed in skeletal muscle. Isoform 2 is primarily expressed in immature thymocytes.
    • Involvement in disease

      Immunodeficiency 42
    • Sequence similarities

      Belongs to the nuclear hormone receptor family. NR1 subfamily.
      Contains 1 nuclear receptor DNA-binding domain.
    • Domain

      The AF-2 (activation function-2) motif is required for recruiting coregulators containing LXXLL motifs such as NCOA1 and NCOA2.
    • Cellular localization

      Nucleus.
    • Target information above from: UniProt accession P51449 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 527470 Cow
      • Entrez Gene: 483205 Dog
      • Entrez Gene: 6097 Human
      • Entrez Gene: 368158 Rat
      • Omim: 602943 Human
      • SwissProt: P51449 Human
      • Unigene: 256022 Human
      • Unigene: 607993 Human
      • Alternative names

        • IMD42 antibody
        • MGC129539 antibody
        • NR1F3 antibody
        • Nuclear receptor ROR gamma antibody
        • Nuclear receptor ROR-gamma antibody
        • Nuclear receptor RZR gamma antibody
        • Nuclear receptor RZR-gamma antibody
        • Nuclear receptor subfamily 1 group F member 3 antibody
        • RAR related orphan nuclear receptor variant 2 antibody
        • RAR related orphan receptor C antibody
        • RAR related orphan receptor C, isoform a antibody
        • RAR related orphan receptor gamma antibody
        • RAR-related orphan receptor C antibody
        • Retinoic acid binding receptor gamma antibody
        • Retinoid related orphan receptor gamma antibody
        • Retinoid-related orphan receptor-gamma antibody
        • Rorc antibody
        • RORG antibody
        • RORG_HUMAN antibody
        • RZR GAMMA antibody
        • RZRG antibody
        • TOR antibody
        see all

      Images

      • Flow Cytometry - FITC Anti-ROR gamma antibody [4G419], prediluted (ab104906)
        Flow Cytometry - FITC Anti-ROR gamma antibody [4G419], prediluted (ab104906)
        Intracellular flow cytometric analysis of ROR gamma in Human lymphocytes using 10µl (0.25 ug) of ab104906. Shaded histogram represents cells without antibody; green represents isotype control; red represents ab104906.

      Protocols

      • Flow cytometry protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (1)

      Publishing research using ab104906? Please let us know so that we can cite the reference in this datasheet.

      ab104906 has been referenced in 1 publication.

      • Pasi S  et al. Toll/Interleukin-1 Receptor Domain Derived from TcpC (TIR-TcpC) Ameliorates Experimental Autoimmune Arthritis by Down-modulating Th17 Cell Response. J Biol Chem 291:12358-69 (2016). PubMed: 27022030

      Customer reviews and Q&As

      Show All Reviews Q&A
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      1-2 of 2 Abreviews or Q&A

      Flow Cytometry abreview for Anti-ROR gamma antibody [4G419], prediluted (FITC)

      Poor
      Abreviews
      Abreviews
      abreview image
      Application
      Flow Cytometry
      Sample
      Rat Cell (Lung)
      Permeabilization
      Yes - Cytofix/Cytoperm BD Cat: 554714
      Gating Strategy
      Singlet Cells -> Viable Cells (Vioblue) -> Leucocytes (CD45+ APCy7) -> CD172 (PE) Negative cells -> CD3 (Viogreen) positive Cells
      Specification
      Lung
      Preparation
      Cell harvesting/tissue preparation method: Bronchoalveolar Lavage
      Sample buffer: PBS + 5% FCS
      Fixation
      Cytofix/Cytoperm BD Cat: 554714
      Read More

      Dr. Eder Fredy Mateus

      Verified customer

      Submitted Aug 17 2017

      Question

      Customer kindly contacted us regarding this product which did not arrive with the correct amount.

      Read More

      Abcam community

      Verified customer

      Asked on Nov 19 2012

      Answer

      Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not arrive with the full volume as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxxxx. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.

      Read More

      Abcam Scientific Support

      Answered on Nov 19 2012

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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